Comparative characterization of mesenchymal bone marrow stromal cells at early and late stages of culturing

Kozhevnikova, M. N.; Mikaelyan, Arsen; Payushina, O. V.; Старостин, В. И.

doi:10.1134/s1062359008020064

Cited by 14 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…doi: 10.7243/2054-717X-3-3 percentage of BrdU-IR cells was observed at P4 (♂). Previous studies reported reduced proliferation and/or growth-arrest in rat MSCs at passage 4 or 5 [41][42][43]. Population doubling time at P4 (♂) in the present study, however, was not significantly different from other passages and only the ratio of BrdU-IR cells decreased.…”

Section: Discussioncontrasting

confidence: 82%

Bone marrow-derived mesenchymal stem cells (MSCs) stimulate neurite outgrowth from differentiating adult hippocampal progenitor cells

Ye¹,

Chawla²,

Khan³

et al. 2016

Stem Cell Biol Res

View full text Add to dashboard Cite

Background: Bone marrow-derived mesenchymal stem cells (MSCs) have emerged as beneficial cellular vehicles for nervous system rescue and repair. A better understanding how MSCs are involved in mediating neural repair will facilitate development of novel therapeutic strategies. Methods: In the present study bone marrow-derived MSCs were isolated and characterized from Brown Norway rats. The capacity of the MSCs to influence the differentiation of adult hippocampal progenitor cells (AHPCs) was investigated using contact and non-contact co-culture configurations. Results: These MSCs showed a stable and consistent growth rate, retained short population doubling time (PDT) and showed high capacity of cell proliferation. Co-culturing of AHPCs with MSCs did not appear to significantly affect the proliferation of the AHPCs or impact the proportion of neuronal or glial differentiation of the AHPCs. However, both contact co-culture (CCC) and non-contact co-culture (NCCC) significantly promoted neurite outgrowth from neuronal AHPCs. Conclusions: The ability of MSCs to promote the morphological differentiation of AHPCs may serve as an added benefit when developing cell-based strategies for nervous system rescue and repair.

show abstract

Section: Discussioncontrasting

confidence: 82%

Bone marrow-derived mesenchymal stem cells (MSCs) stimulate neurite outgrowth from differentiating adult hippocampal progenitor cells

Ye¹,

Chawla²,

Khan³

et al. 2016

Stem Cell Biol Res

View full text Add to dashboard Cite

show abstract

“…These data are consistent with previous reports showing the gradual commitment of late‐passage MSCs to the osteogenic lineage (18, 19, 59). A previous study also reported increased Runx2 expression in late‐passage MSCs (60). In addition to an increase in Runx2, a decrease in the expression of stemness markers, such as Sox2 and Oct4, has been reported in senescent MSCs (35).…”

Section: Discussionmentioning

confidence: 56%

Interleukin‐6 induces the lineage commitment of bone marrow‐derived mesenchymal multipotent cells through down‐regulation of Sox2 by osteogenic transcription factors

et al. 2014

View full text Add to dashboard Cite

Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are a heterogeneous population of cells that differ in size and morphology. BM-MSCs become committed to the osteogenic lineage as senescence approaches and lose multipotency. Nevertheless, little is known about the effects of cell-cell interaction between different populations on stemness loss and lineage commitment. The current study aimed to identify mechanisms by which cell-cell interactions between heterogeneous BM-MSCs affect stemness and lineage commitment of multipotent subpopulation. The lineage commitment of primitive multipotent cells was strongly induced in the presence of cytokines secreted by senescent-like cells in a cell culture insert system. Senescent-like cells secreted higher levels of interleukin-6 (IL-6) than primitive multipotent cells in a human cytokine array. IL-6 induced the lineage commitment and stemness loss in multipotent cells by decreasing Sox2 expression. Furthermore, we confirmed that IL-6 decreased the transcriptional activity of Sox2 through up-regulation of Runx2 and Dlx5. We suggest a mechanism by which IL-6 modulates the expression of Sox2, resulting in decreased multipotency and causing primitive multipotent cells to undergo osteogenic lineage commitment. This is the first study to identify mechanisms in which the cell-cell interactions between the different populations play important roles in the stemness loss and lineage commitment of multipotent populations.-Yoon, D. S., Kim, Y. H., Lee, S., Lee, K.-M., Park, K. H., Jang, Y., Lee, J. W. Interleukin-6 induces the lineage commitment of bone marrow-derived mesenchymal multipotent cells through down-regulation of Sox2 by osteogenic transcription factors.

show abstract

“…5b). Although multi‐differentiation ability of BM‐derived MSCs decreases after expansion in culture, potential for osteogenic differentiation is retained or increased (15,16,37). These reports indicate that MSCs differentiate into osteogenic cells with continued cell passaging.…”

Section: Resultsmentioning

confidence: 99%

Importance of Sox2 in maintenance of cell proliferation and multipotency of mesenchymal stem cells in low-density culture

Yoon¹,

Kim²,

Jung³

et al. 2011

Cell Proliferation

106

View full text Add to dashboard Cite

We repopulated 'primitive' cells by replating late-passage MSCs at low density (17 cells/cm(2) ) regardless of donor age. Repopulated MSCs from low-density culture were smaller cells with spindle shaped morphology compared to MSCs from high-density culture. The latter had enhanced colony-forming ability, proliferation rate, and adipogenic and chondrogenic potential. Strong expression of osteogenic-related genes (Cbfa1, Dlx5, alkaline phosphatase and type Ι collagen) in late-passage MSCs was reduced by replating at low density, whereas expression of three pluripotency markers (Sox2, Nanog and Oct-4), Osterix and Msx2 reverted to levels of early-passage MSCs. Knockdown of Sox2 and Msx2 but not Nanog, using RNA interference, showed significant decrease in colony-forming ability. Specifically, knockdown of Sox2 significantly inhibited multipotentiality and cell proliferation. Our data suggest that plating density should be considered to be a critical factor for enrichment of 'primitive' cells from heterogeneous BM and that replicative senescence and multipotentiality of MSCs during in vitro expansion may be predominantly regulated through Sox2.

show abstract

Comparative characterization of mesenchymal bone marrow stromal cells at early and late stages of culturing

Cited by 14 publications

References 15 publications

Bone marrow-derived mesenchymal stem cells (MSCs) stimulate neurite outgrowth from differentiating adult hippocampal progenitor cells

Bone marrow-derived mesenchymal stem cells (MSCs) stimulate neurite outgrowth from differentiating adult hippocampal progenitor cells

Interleukin‐6 induces the lineage commitment of bone marrow‐derived mesenchymal multipotent cells through down‐regulation of Sox2 by osteogenic transcription factors

Importance of Sox2 in maintenance of cell proliferation and multipotency of mesenchymal stem cells in low-density culture

Contact Info

Product

Resources

About