Comparative characterization of human fetal neural stem cells and induced neural stem cells from peripheral blood mononuclear cells

Tang, Xihe; Yu, Meigang; Huang, Ri-Zhen; Lan, Shengyong; Yang, Fan

doi:10.32604/biocell.2020.07593

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…To explore the potential functions of the eight E2Fs in GBM, several bioinformatics tools were applied to determine the associations between E2Fs expression patterns and the clinical characteristics of samples from the TCGA and GSE79671 datasets. A protein-protein interaction (PPI) network of the eight E2Fs and other differentially expressed (DE) genes was constructed using the STRING database, Cytoscape software, and Bioinformatics [17,21]. For E2Fs expression in GBM patients and normal individuals, DoSurvive and its specimens were used [22].…”

Section: Bioinformatics Analysismentioning

confidence: 99%

Early 2-Factor Transcription Factors Associated with Progression and Recurrence in Bevacizumab-Responsive Subtypes of Glioblastoma

Shi

2024

Preprint

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The early 2-factor (E2F) family of transcription factors, including E2F1-8, plays a critical role in apoptosis, metabolism, proliferation, and angiogenesis within glioblastoma (GBM). However, the specific functions of E2F transcription factors (E2Fs) and their impact on the malignancy of Bevacizumab (BVZ)-responsive GBM subtypes remain unclear. This study used data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) to explore the impact of eight E2F family members on the clinical characteristics of BVZ-responsive GBM subtypes and possible mechanisms of recurrence after BVZ treatment.To predict the possibility of GBM patient survival and progression, we classified and comparedthe expression of E2Fs according to BVZ-responsive subtypes and employed a machine learning method-TreeBagger, one random forest algorithm. Multiple bioinformatics analyses have shown that the significant increased E2F8 post BVZ treatment may enhance the function of angiogenesis and stem cell proliferation, indicating one of the mechanisms of GBM recurrence after treatment. In addition, BVZ treatment in unresponsive GBM patients may potentially worsen disease progression. Our findings suggest that E2F family members play important roles in GBM malignancy and BVZ treatment response, highlighting their potential as prognostic biomarkers and therapeutic targets and recommending precise BVZ treatment for GBM patients.

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Section: Bioinformatics Analysismentioning

confidence: 99%

Early 2-Factor Transcription Factors Associated with Progression and Recurrence in Bevacizumab-Responsive Subtypes of Glioblastoma

Shi

2024

Preprint

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“…Satellite cells are usually mitotically quiescent but can be activated upon injury, leading to the production of myogenic precursor cells or myoblasts to promote muscle regeneration [8]. Stem cells are known to have great potential of differentiation and regeneration [9,10]. Satellite cells are the primary myogenic cells that contribute to skeletal muscle regeneration after muscle injury [11,12].…”

Section: Introductionmentioning

confidence: 99%

Mechanical ventilation regulated the differentiation and proliferation of diaphragm satellite cells

Ding

Liang

et al. 2021

Front Biosci (Landmark Ed)

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Aim: This study aimed to determine the effect of mechanical ventilation (MV) on the differentiation and proliferation of diaphragm satellite cells. Methods: Diaphragm satellite cells were isolated from C57 mice receiving 6 h of MV with optimized magnetic-activated cell sorting (MACS) approach. The cells were stained with BrdU or antibody for differentiation marker MYH3. The expression of MyoD and myogenin was detected by realtime PCR. Results: Diaphragm satellite cells were successfully isolated from mice by using MACS with a set of optimized parameters. About 1.5 × 10 5 cells could be harvested from a diaphragm. Upon MV, the proliferation rate of diaphragm satellite cells was decreased from 88.74% to 81.92%, while the differentiation rate was increased from 17.94% to 27.58%, compared to controls. Moreover, the expression of MyoD and myogenin were significantly upregulated upon MV. Conclusions: We established a practical method to purify diaphragm satellite cells, and demonstrated that MV regulated the differentiation and proliferation of diaphragm satellite cells.

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Comparative characterization of human fetal neural stem cells and induced neural stem cells from peripheral blood mononuclear cells

Cited by 2 publications

References 26 publications

Early 2-Factor Transcription Factors Associated with Progression and Recurrence in Bevacizumab-Responsive Subtypes of Glioblastoma

Early 2-Factor Transcription Factors Associated with Progression and Recurrence in Bevacizumab-Responsive Subtypes of Glioblastoma

Mechanical ventilation regulated the differentiation and proliferation of diaphragm satellite cells

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