Comparative characteristics of the VP7 and VP4 antigenic epitopes of the rotaviruses circulating in Russia (Nizhny Novgorod) and the Rotarix and RotaTeq vaccines

Морозова, О. В.; Сашина, Т. А.; Фомина, С Г; Новикова, Н. А.

doi:10.1007/s00705-015-2439-6

Cited by 32 publications

(26 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cytotoxic T lymphocytes (CTL) have been linked to clearance of RV infection resulting in complete short-term and partial long-term protection against re-infection 27 . Two linear CTL epitopes responsible for this activity have been reported on VP7 protein at amino acid positions 16-28 and 40-52 28,29 . An analyses of the Rwandan G1 pre-and post-vaccine introduction strains showed three amino acid differences at positions T41F/S, V42M and A46V with G1 component of the RotaTeq and Y41F/S, V42M and A46V to Rotarix vaccines ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Whole genome and in-silico analyses of G1P[8] rotavirus strains from pre- and post-vaccination periods in Rwanda

Rasebotsa

Mwangi

Mogotsi

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Rwanda was the first low-income African country to introduce RotaTeq vaccine into its Expanded Programme on Immunization in May 2012. To gain insights into the overall genetic make-up and evolution of Rwandan G1P[8] strains pre-and post-vaccine introduction, rotavirus positive fecal samples collected between 2011 and 2016 from children under the age of 5 years as part of ongoing surveillance were genotyped with conventional RT-PCR based methods and whole genome sequenced using the Illumina MiSeq platform. From a pool of samples sequenced (n = 158), 36 were identified as G1P[8] strains (10 pre-vaccine and 26 post-vaccine), of which 35 exhibited a typical Wa-like genome constellation. However, one post vaccine strain, RVA/Human-wt/RWA/UFS-NGS:MRC-DPRU442/2012/ G1P[8], exhibited a RotaTeq vaccine strain constellation of G1-P[8]-I2-R2-C2-M2-A3-N2-T6-E2-H3, with most of the gene segments having a close relationship with a vaccine derived reassortant strain, previously reported in USA in 2010 and Australia in 2012. The study strains segregated into two lineages, each containing a paraphyletic pre-and post-vaccine introduction sub-lineages. In addition, the study strains demonstrated close relationship amongst each other when compared with globally selected group A rotavirus (RVA) G1P[8] reference strains. For VP7 neutralization epitopes, amino acid substitutions observed at positions T91A/V, S195D and M217T in relation to the RotaTeq vaccine were radical in nature and resulted in a change in polarity from a polar to non-polar molecule, while for the VP4, amino acid differences at position D195G was radical in nature and resulted in a change in polarity from a polar to non-polar molecule. The polarity change at position T91A/V of the neutralizing antigens might play a role in generating vaccine-escape mutants, while substitutions at positions S195D and M217T may be due to natural fluctuation of the RVA. Surveillance of RVA at whole genome level will enhance further assessment of vaccine impact on circulating strains, the frequency of reassortment events under natural conditions and epidemiological fitness generated by such events. Group A rotavirus is a significant viral etiological agents of acute gastroenteritis (AGE) resulting in approximately 125,000 deaths annually in children under 5 years worldwide 1. Rwanda rolled out the RotaTeq (Merck and Co., Whitehouse Station, NJ, USA) vaccine into her Expanded Program on Immunization in May 2012 and the vaccination coverage rate has been consistently high over the years > 95% since 2013, resulting in significant

show abstract

Section: Resultsmentioning

confidence: 99%

Whole genome and in-silico analyses of G1P[8] rotavirus strains from pre- and post-vaccination periods in Rwanda

Rasebotsa

Mwangi

Mogotsi

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Strains isolated from Russia (RVA/Human-wt/RUS/NN2406/2011/G2P[4] and RVA/Human-wt/RUS/NN2578/2011/G2P[4]) had the same S242N change in the VP7 epitope (7-1b) as RVA/Human-wt/MOZ/0308/2012/G2P[4], which is also similar to RotaTeq ® (SC2-9) G2 [ 39 ]. These strains formed a sub-cluster in lineage IV, separate from other G2P[4] strains (Fig.…”

Section: Discussionmentioning

confidence: 99%

Rotavirus A strains obtained from children with acute gastroenteritis in Mozambique, 2012-2013: G and P genotypes and phylogenetic analysis of VP7 and partial VP4 genes

et al. 2017

View full text Add to dashboard Cite

In Mozambique rotavirus (RV) was shown to be the greatest cause of acute diarrhoea in infants from 0 to 11 months, and in 2015, national rotavirus vaccination was introduced. As with other developing countries, there is very limited active strain characterisation. Rotavirus positive clinical specimens, collected between 2012 and 2013, have now provided information on the genotypes circulating in southern Mozambique prior to vaccine introduction. Genotypes G2 (32.4%), G12 (28.0%), P[4] (41.4%) and P[6] (22.9%) (n = 157) strains were commonly detected with G2P[4] (42.3%) RVs being predominant, specifically during 2013. Phylogenetic evaluation of the VP7 and VP8* encoding genes showed, for the majority of the Mozambican strains, that they clustered with other African strains based on genotype. RVA/Human-wt/MOZ/0153/2013/G2P[4], RVA/Human-wt/MOZ/0308/2012/G2P[4] and RVA/Human-wt/MOZ/0288/2012/G12P[8] formed separate clusters from the other Mozambican strains with similar genotypes, suggesting possible reassortment. Amino acid substitutions in selected epitope regions also supported phylogenetic clustering. As expected, the VP7 and VP8* genes from the Mozambican strains differed from both the RotaTeq® (SC2-9) G2P[5] and Rotarix® (A41CB052A) G1P[8] genes. This study provides information on the genetic diversity of rotavirus strains prior to vaccine introduction and generates baseline data for future monitoring of any changes in rotavirus strains in response to vaccine pressure.Electronic supplementary materialThe online version of this article (doi:10.1007/s00705-017-3575-y) contains supplementary material, which is available to authorized users.

show abstract

“…The two established VP7 cytotoxic T-cell lymphocyte epitopes at positions 16-28 and 40-52 [44] of the VP7 of South African G1P [8] strains were compared with cognate regions of Rotarix ® VP7. Five amino acid differences appeared before Rotarix ® introduction, while two amino acid substitutions (A43V in two strains and A46T in four strains) were found after vaccine introduction (Table S1 in S6).…”

Section: Comparative Analysis Of Vp7 Cytotoxic T Lymphocyte Epitopes mentioning

confidence: 99%

Whole Genome In-Silico Analysis of South African G1P[8] Rotavirus Strains before and after Vaccine Introduction over a Period of 14 Years

et al. 2020

View full text Add to dashboard Cite

Rotavirus G1P[8] strains account for more than half of the group A rotavirus (RVA) infections in children under five years of age, globally. A total of 103 stool samples previously characterized as G1P[8] and collected seven years before and seven years after introducing the Rotarix® vaccine in South Africa were processed for whole-genome sequencing. All the strains analyzed had a Wa-like constellation (G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1). South African pre- and post-vaccine G1 strains were clustered in G1 lineage-I and II while the majority (84.2%) of the P[8] strains were grouped in P[8] lineage-III. Several amino acid sites across ten gene segments with the exception of VP7 were under positive selective pressure. Except for the N147D substitution in the antigenic site of eight post-vaccine G1 strains when compared to both Rotarix® and pre-vaccine strains, most of the amino acid substitutions in the antigenic regions of post-vaccine G1P[8] strains were already present during the pre-vaccine period. Therefore, Rotarix® did not appear to have an impact on the amino acid differences in the antigenic regions of South African post-vaccine G1P[8] strains. However, continued whole-genome surveillance of RVA strains to decipher genetic changes in the post-vaccine period remains imperative.

show abstract

Comparative characteristics of the VP7 and VP4 antigenic epitopes of the rotaviruses circulating in Russia (Nizhny Novgorod) and the Rotarix and RotaTeq vaccines

Cited by 32 publications

References 37 publications

Whole genome and in-silico analyses of G1P[8] rotavirus strains from pre- and post-vaccination periods in Rwanda

Whole genome and in-silico analyses of G1P[8] rotavirus strains from pre- and post-vaccination periods in Rwanda

Rotavirus A strains obtained from children with acute gastroenteritis in Mozambique, 2012-2013: G and P genotypes and phylogenetic analysis of VP7 and partial VP4 genes

Whole Genome In-Silico Analysis of South African G1P[8] Rotavirus Strains before and after Vaccine Introduction over a Period of 14 Years

Contact Info

Product

Resources

About