Comparative bioactivities of mastoparans from social hornets Vespa crabro and Vespa analis

Yoon, Kyungjae Andrew; Kim, Kyungmun; Nguyen, Phuong; Seo, Jong Bok; Park, Young Han; Kim, Ki-Gyoung; Seo, Hong-Yul; Koh, Young Ho; Lee, Si Hyeock

doi:10.1016/j.aspen.2015.10.006

Cited by 19 publications

(19 citation statements)

References 13 publications

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“…Total reads of 5.89, 6.39, 7.13, 5.74, 6.22, 6.16, 11.3, 11.4, 8.2, 8.07, 8.1, 7.66, 10.9, and 10.6 Gb were obtained from RNA sequencing of the venom glands of Eumenes decoratus, Sphecidae sp., Anterhynchium flavomarginatum, Sceliphron deforme, V. crabro, V. analis, V. dybowskii, V. simillima, Parapolybia varia, Polistes snelleni, Polistes rothneyi, Bombus ardens, B. consobrinus, and B. ussurensis, respectively. De novo assembly of the trimmed sequence data resulted in 18,062,31,134,21,512,24,483,11,097,12,531,21,227,29,150,20,378,27,742,25,436,31,366,21,625, and 26,425 assembled contigs, respectively. A total of 16,357, 27,423, 19,033, 20,097, 10,321, 11,284, 19,627, 27,065, 18,539, 25,879, 23,475, 26,158, 20,050, and 22,619 genes, respectively, were identified by BLAST search (Table 1).…”

Section: Differential Transcriptional Profiles Of the Venom Glands Ofmentioning

confidence: 99%

“…V. crabro mastoparan showed an extremely high TPM value among all venom genes in that species. Mastoparan causes allergic inflammation and mast cell degranulation in vertebrates [21]. Therefore, social wasp venoms containing relatively high levels of mastoparan are likely to be highly allergenic to vertebrates (Figure 1).…”

Section: Venom Proteins Highly Expressed In Social Waspsmentioning

confidence: 99%

“…Venom components and their abundance in bee and wasp venom have mostly been studied in individual species [5,20]. Only a few studies have compared the components in venoms of two closely-related wasp species [21,22]. A comparative analysis of venom gland transcriptomes of two closely-related social hornet species (Vespa crabro and V. analis) revealed similar overall venom components, but most of the major venom component genes were more abundantly transcribed in V. crabro [21].…”

Section: Introductionmentioning

confidence: 99%

“…Only a few studies have compared the components in venoms of two closely-related wasp species [21,22]. A comparative analysis of venom gland transcriptomes of two closely-related social hornet species (Vespa crabro and V. analis) revealed similar overall venom components, but most of the major venom component genes were more abundantly transcribed in V. crabro [21]. However, little attempt has been made for the systematic analysis and comparison of the venom components in multiple bee and wasp species.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Venom Components and Their Phylogenetic Properties in Some Aculeate Bumblebees and Wasps

Yoon

Kim

Kim³

et al. 2020

Toxins

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To identify and compare venom components and expression patterns, venom gland-specific transcriptome analyses were conducted for 14 Aculeate bees and wasps. TPM (transcripts per kilobase million) values were normalized using the average transcription level of a reference housekeeping gene (dimethyladenosine transferase). Orthologous venom component genes across the 14 bee and wasp species were identified, and their relative abundance in each species was determined by comparing normalized TPM values. Based on signal sequences in the transcripts, the genes of novel venom components were identified and characterized to encode potential allergens. Most of the allergens and pain-producing factors (arginine kinase, hyaluronidase, mastoparan, phospholipase A1, phospholipase A2, and venom allergen 5) showed extremely high expression levels in social wasps. Acid phosphatase, neprilysin, and tachykinin, which are known allergens and neurotoxic peptides, were found in the venom glands of solitary wasps more often than in social wasps. In the venom glands of bumblebees, few or no transcripts of major allergens or pain-producing factors were identified. Taken together, these results indicate that differential expression patterns of the venom genes in some Aculeate species imply that some wasps and bumblebee species have unique groups of highly expressed venom components. Some venom components reflected the Aculeate species phylogeny, but others did not. This unique evolution of specific venom components in different groups of some wasps and bumblebee species might have been shaped in response to both ecological and behavioral influences.

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Section: Differential Transcriptional Profiles Of the Venom Glands Ofmentioning

confidence: 99%

Section: Venom Proteins Highly Expressed In Social Waspsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of Venom Components and Their Phylogenetic Properties in Some Aculeate Bumblebees and Wasps

Yoon

Kim

Kim³

et al. 2020

Toxins

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“…The components of RIFA venom are quite different from those of other hymenopterans. The major components of the venom in bees and wasps include proteins, mature peptides usually shorter than 100 amino acids in length and small molecules, such as amino acids, amines, carbohydrates, and lipids (Lima & Brocheto‐Braga, ; Yoon et al, ). In contrast, more than 90% of RIFA venom consists of alkaloids, such as solenopsin.…”

Section: Introductionmentioning

confidence: 99%

Developing molecular diagnostics for detection of red imported fire ants using two genes, Sinv11108 and Sinv11977

Kim

Park

Kim³

et al. 2019

Arch Insect Biochem Physiol

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Aggressive red imported fire ants (RIFAs) are expanding their habitat due to active international trade and global warming. To prevent infestation and settlement, RIFAs must be removed during the quarantine process. Because RIFAs are social insects and have different morphological characteristics depending on their castes, non-ant taxonomists have difficulty confirming RIFAs based on their morphological characteristics alone. The disadvantages of previously reported RIFA molecular diagnostics are that they require additional steps, such as restriction enzyme digestion followed by agarose gel electrophoresis separation or DNA sequence verification for polymerase chain reaction (PCR)amplified products. To overcome these drawbacks, two RIFA-specific genes were selected and used to develop diverse PCR-based RIFA molecular diagnostic techniques. We found that RIFAs could be confirmed by conventional PCR targeting of two RIFA-specific genes followed by agarose electrophoresis separation. In addition, TaqMan probe real-time PCR methods had the advantage of confirming RIFAs immediately after the reactions were completed by observing fluorescence indexes. Finally, Ji-Hye Kim and Youngjin Park contributed equally to this study.multiplex PCRs enhanced RIFA specificity and sensitivity.The new molecular diagnostic methods developed in this study had the advantages of reducing false positive and negative results together with high specificity and sensitivity for RIFAs.

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Sericulture and the edible-insect industry can help humanity survive: insects are more than just bugs, food, or feed

Park¹,

Kim

Baik

et al. 2022

Food Sci Biotechnol

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Comparative bioactivities of mastoparans from social hornets Vespa crabro and Vespa analis

Cited by 19 publications

References 13 publications

Characterization of Venom Components and Their Phylogenetic Properties in Some Aculeate Bumblebees and Wasps

Characterization of Venom Components and Their Phylogenetic Properties in Some Aculeate Bumblebees and Wasps

Developing molecular diagnostics for detection of red imported fire ants using two genes, Sinv11108 and Sinv11977

Sericulture and the edible-insect industry can help humanity survive: insects are more than just bugs, food, or feed

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