Comparative Banding Pattern Analyses in Human Chromosomes Induced by Urea and Trypsin Treatments

Shiraishi, Yukimasa; Yosida, Tosihide H.

doi:10.1266/jjg.48.11

Cited by 4 publications

(1 citation statement)

References 11 publications

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“…Chromosome banding studies were carried out according to G-, Q-and C-bandings (Shiraishi and Yoshida 1973;Caspersson et a1.1972;Sumner 1972). Chromosomal aberrations were counted in 100-200 metaphases of the irradiated cells.…”

Section: Methodsmentioning

confidence: 99%

Radiation sensitivity and chromosomal changes in human leukemic T-cell lines.

Taguchi

Shiraishi

1983

The Japanese journal of genetics

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Radiosensitive and resistant cell lines were studied with regard to radiation survival and chromosomal changes. A radioresistant cell clone (CCRF-HSB2-M) was spontaneously separated from a radiosensitive human T-cell line (CCRF-HSB2) which was originated from the peripheral blood of the patient with an acute lymphoblastic leukemia (ALL). Cell growth and dose-survival curves after X-irradiation indicated that HSB2-M cells were as resistant as normal cells, though the frequency of chromosomal aberrations in irradiated HSB2-M cells was slightly higher than that in irradiated normal cells. Higher frequencies of chromatid and chromosome deletions (breaks) were observed in irradiated HSB2 cells, whereas those of deletions in HSB2-M and normal cells showed a relatively low frequency and the chromosomal aberrations in these two cell lines decreased with time. The karyotype of radiosensitive HSB2 cells was XY, 46, t(1; 7) (p 32; q 32). The radioresistant mutant clone (HSB2-M) gained some other changes in addition to the original translocation; 47, XY, t (1; 7) (p 32; q 32), + t(6; 6) (p 11; q 13), t(12;16) (q 24; q 11), -16 p, +22. The acquirement of radioresistant character in HSB2-M cells could be closely related to the additional chromosomal changes (+iso6q and +22). The emergence of a radioresistant clone from a radiosensitive cell line is discussed in relation to human leukemia.

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Section: Methodsmentioning

confidence: 99%