Comparative assessment of virulence traits in Legionella spp.

Alli, O. A. Terry; Zink, Steven D.; Lackum, N. Katherine von; Kwaik, Yousef Abu

doi:10.1099/mic.0.25980-0

Cited by 54 publications

(51 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This strategy pruned down the number of bacterial isolates down to 5, of which all of them were Gram negative rods exhibiting both catalase and oxidase activities with hippurate hydrolysis and gelatine liquefaction -a biochemical result suggestive of Legionella spp. Alli et al (2003) have shown before that pore forming activity within the genus Legionella is associated with L. pneumophila and L. spirintensis. It was in view of this that pore forming activity was tested for in all the 5 suspected legionellae isolates by performing haemolysis assay in which sheep red blood cells were used, the result (Figure 1) showed that all exhibited pore forming activity that was very close to that of L. pneumophila AA100 that was used as a positive control.…”

Section: Resultsmentioning

confidence: 99%

“…These biochemical tests combined with demonstration of pore forming activity of L. pneumophila helped in narrowing down to L. pneumophila. Pore forming activity has been described as one of the virulence traits of L. pneumophila (Alli et al, 2003) which is best demonstrated by haemolysis assay using sheep red blood cells. Combining this haemolysis assay and the ability to amplify the 0.52 kb portion of the dot/icm region of L. pneumophila helped in the final identification.…”

Section: Discussionmentioning

confidence: 99%

“…sheep red blood cells (sRBC) by suspected Legionellae isolates as previously described (Alli et al, 2003). Briefly, sheep red blood cells (sRBC) were washed three times by centrifugation for 10 min at 500 x g until a clear supernatant was seen; the cells were then counted with a haemocytometer.…”

Section: Pore Forming Activitymentioning

confidence: 99%

“…Apoptosis and necrosis have been shown to contribute to cell death of the host cells (Gao and Abu Kwaik, 1999;2000). The dot/icm loci have been demonstrated in all species of Legionellae to date by hybridisation (Alli et al, 2003), however, the functionality of these loci are in doubt.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Isolation of Legionella pneumophila from surface and ground waters in Osogbo, Nigeria

Alli¹,

Olusoga²,

Adedokun³

et al. 2011

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Legionella is a ubiquitous water environmental organism. Isolation/detection of Legionellae has been reported worldwide. However, there is no reported isolation of Legionellae in countries in Africa with the exception of South Africa. The aim of this study was to survey the surface, ground waters and air conditioner water systems (ACWs) in Osogbo, Nigeria for the presence of Legionella pneumophila by cultural isolation method. A total of 313 water samples from the streams (surface water), wells (groundwater) and ACWS were cultured on buffered charcoal yeast extract (BCYE) medium. The isolates were identified by Gram staining techniques, biochemical tests and demonstration of pore forming activity. Polymerase chain reaction for amplification of 0.52 kb fragment of the part of dot/icm region of L. pneumophila was used for the final identification. The results obtained showed that the prevalence of L. pneumophila from streams (8%) was statistically significant when compared to wells (1%) (Chi square, P < 0.05). L. pneumophila was not isolated from any of the water samples taken from air-conditioner water systems. The study concludes that L. pneumophila is present in natural aquatic environment in Nigeria.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Pore Forming Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation of Legionella pneumophila from surface and ground waters in Osogbo, Nigeria

Alli¹,

Olusoga²,

Adedokun³

et al. 2011

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…L. pneumophila strain AA100/130b (BAA-74; American Type Culture Collection) and the isogenic mutants dotA, ankB, and complemented ankB mutants were grown as described previously . The L. longbeachae strains and L. micdadei and L. dumoffii strains (Alli et al, 2003) have been previously described. E. coli strain DH5- was used for cloning purposes.…”

Section: Methodsmentioning

confidence: 99%