Comparative assessment of organic solvent extraction on non-specific immune defences of skin mucus from freshwater fish

Sridhar, Arun; Guardiola, Francisco A.; Sekar, Rajkumar Krishnasamy; Murugesan, S.; Palaniyappan, Sivagaami; Manikandan, Dinesh Babu; Arumugam, Manohar; Ramasamy, Thirumurugan

doi:10.1007/s10499-022-00847-1

Cited by 5 publications

(3 citation statements)

References 78 publications

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“…This might be due to better environmental conditions and interaction with multiple organisms. The mucus effect varies among the species (Sridhar et al., 2022). Also, in the current study, mucus extraction effect was higher in pond fish.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the antibacterial activity and protein profiling of Nile tilapia (Oreochromis niloticus) epidermal mucus under different feeds and culture systems (biofloc technology and earthen pond)

Habib,

Batool,

Rehman

et al. 2023

Journal of Fish Diseases

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The mucus layers of fish serve as the main interface between the organism and the environment. They play an important biological and ecological role. The current study focuses on Nile tilapia epidermal mucus reared under different commercial feeds (coded A and B) and environments (biofloc technology and earthen pond systems). Crude protein levels in feed A and B were 30% and 28%, respectively. Water parameters in all culturing systems were suitable for tilapia throughout the study period. The antimicrobial potency of tilapia (n = 5 from each) epidermal mucus was tested in vitro against human and fish pathogenic strains viz. Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Francisella noatunensis, and Aeromonas hydrophila. To determine the antimicrobial activity, zones of inhibition (ZOI) were measured in millimetres and compared with two antibiotics (chloramphenicol and ciprofloxacin). SDS‐PAGE analysis was performed on skin mucus samples of tilapia to determine protein quantity and size (molecular weight). Results of tilapia skin mucus (crude and aqueous) revealed a strong antibacterial effect against all the selected pathogenic strains. However, variation has been observed in the mucus potency and ZOI values between the biofloc and pond tilapia mucus. The crude mucus of tilapia fed on feed A and cultured in the pond exhibited strong antibacterial effects and high ZOI values compared to the mucus of biofloc tilapia, aqueous mucus extracts and positive control chloramphenicol (antibiotic). The SDS‐PAGE results showed that the high molecular weight proteins were found in the collected epidermal mucus of BFT‐B (240 kDa) and EP‐B (230 kDa). Several peptides in fish skin mucus may play a crucial role in the protection of fish against disease‐causing pathogens. Thus, it can be utilized in the human and veterinary sectors as an ‘antimicrobial’ for treating various bacterial infections.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the antibacterial activity and protein profiling of Nile tilapia (Oreochromis niloticus) epidermal mucus under different feeds and culture systems (biofloc technology and earthen pond)

Habib,

Batool,

Rehman

et al. 2023

Journal of Fish Diseases

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show abstract

“…Moreover, Sridhar et al studied the effect of organic solvent extraction on skin mucus of three freshwater fishes, rohu ( Labeo rohita ), carp ( Cyprinus carpio ), and mrigal ( Cirrhinus mrigala ). Results showed that the protease activity observed in the methanol extracts of skin mucus was significantly higher than that of acetone extracts, and the methanol extract of mrigal skin mucus exhibited the highest protease activity . The resolution of this method is higher than that of salt extraction, but the extracted proteins are easily denatured and should be performed at low temperatures.…”

Section: Extraction Methods For Protein From Aquatic Sourcesmentioning

confidence: 99%

“…Results showed that the protease activity observed in the methanol extracts of skin mucus was significantly higher than that of acetone extracts, and the methanol extract of mrigal skin mucus exhibited the highest protease activity. 23 The resolution of this method is higher than that of salt extraction, but the extracted proteins are easily denatured and should be performed at low temperatures.…”

Section: Water Extractionmentioning

confidence: 99%

Current Research on the Extraction, Functional Properties, Interaction with Polyphenols, and Application Evaluation in Delivery Systems of Aquatic-Based Proteins

Han

Jiang

Wang

et al. 2022

J. Agric. Food Chem.

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Globally, aquatic processing industries pay great attention to the production of aquatic proteins for the fulfillment of the nutritive requirements of human beings. Aquatic protein can replace terrestrial animal protein due to its high protein content, complete amino acids, unique flavor, high quality and nutritional value, and requirements of religious preferences. Due to the superior functional properties, an aquatic protein based delivery system has been proposed as a novel candidate for improving the absorption and bioavailability of bioactive substances, which might have potential applications in the food industry. This review outlines the extraction techniques for and functional properties of aquatic proteins, summarizes the potential modification technologies for interaction with polyphenols, and focuses on the application of aquatic-derived protein in delivery systems as well as their interaction with the gastrointestinal tract (GIT). The extraction techniques for aquatic proteins include water, salt, alkali/acid, enzyme, organic solvent, and ultrasound-assisted extraction. The quality and functionality of the aquatic proteins could be improved after modification with polyphenols via covalent or noncovalent interactions. Furthermore, some aquatic protein based delivery systems, such as emulsions, gels, films, and microcapsules, have been reported to enhance the absorption and bioavailability of bioactive substances by in vitro GIT, cell, and in vivo animal models. By promoting comprehensive understanding, this review is expected to provide a real-time reference for developing functional foods and potential food delivery systems based on aquatic-derived proteins.

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Potentials of Aloe barbadensis inclusion in fish feeds on resilience to Aeromonas hydrophila infection in freshwater fish Labeo rohita

Palaniyappan,

Sridhar,

Kari

et al. 2023

Fish Physiol Biochem

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Comparative assessment of organic solvent extraction on non-specific immune defences of skin mucus from freshwater fish

Cited by 5 publications

References 78 publications

Evaluation of the antibacterial activity and protein profiling of Nile tilapia (Oreochromis niloticus) epidermal mucus under different feeds and culture systems (biofloc technology and earthen pond)

Evaluation of the antibacterial activity and protein profiling of Nile tilapia (Oreochromis niloticus) epidermal mucus under different feeds and culture systems (biofloc technology and earthen pond)

Current Research on the Extraction, Functional Properties, Interaction with Polyphenols, and Application Evaluation in Delivery Systems of Aquatic-Based Proteins

Potentials of Aloe barbadensis inclusion in fish feeds on resilience to Aeromonas hydrophila infection in freshwater fish Labeo rohita

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