Comparative assessment of immunochromatography and ELISA diagnostic tests for HBsAg detection in PCR-confirmed HBV infection

Navvabi, Nazila; Ansari, Mohammad Hassan Khadem; Navvabi, Azita; Chalipa, H.R.; Zitricky, Frantisek

doi:10.1016/j.rgmxen.2021.11.007

Cited by 6 publications

(7 citation statements)

References 15 publications

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“…By making a comparison between the cassette and ELISA techniques, the present result showed that the ELISA technique has more sensitivity and specificity HBsAg detection. This finding has been supported in some reports 47, 48 .…”

Section: Discussionsupporting

confidence: 86%

Seroprevalence of the Viral Markers of Hepatitis B, Hepatitis C, and Hiv Among Medical Waste Handlers in Some Hospitals in Sana'a City- Yemen

Edrees

Alrahabi

et al. 2022

Univ J Pharm Res

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Background and Objective: Hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV) are highly contagious pathogens that threaten medical waste handlers who are highly exposed to these viruses due to the nature of their work. To date, little data is available on the prevalence of viral markers (HBV, HCV, and HIV) among medical waste handlers in Sana'a city, Yemen. Hence, this work was conducted to enhance knowledge of the prevalence of viral markers among medical waste handlers working in some hospitals in the capital, Sana'a. Methods: A cross-sectional prospective study was conducted on 120 randomly selected medical waste handlers from January to June 2022. A pretested and designed questionnaire was used to collect the required data. Total 5mL of venous blood was collected. Then blood was tested for hepatitis B surface antigen and anti-HCV, as well as HIV antibody, were detected using a qualitative by rapid test cassette and positive results were confirmed by ELIZA technique. Results: The overall positive rates for HBsAg, anti-HCV, and HIV were 9.17%, 5.0%, and 0.0%, respectively, based on the rapid test. No significant differences were detected between socio-demographic characteristics and hepatitis B and HCV infection. High rates of hepatitis B and C infection were found in the age group ≥41 years (9.17%) and 21-30 years (8.77%), respectively. These two viruses have also been detected among married people with a history of acute infection between 7-9 times. There was a significant correlation between HBsAg positivity and history of blood transfusion as well as between HBsAg and anti-HCV positivity and history of hepatitis B vaccination. ELISA confirmed that the rate of HBsAg was 4.17% and anti-HCV was 2.5%. Conclusion: Appropriate training, immunization against HBV, and regular motivation of medical waste handlers on the practices of universal standard precautions are recommended to reduce transmission of hepatitis B virus, hepatitis C virus, and HIV among medical waste handlers. Peer Review History: Received: 4 May 2022; Revised: 14 June; Accepted: 27 June, Available online: 15 July 2022 Academic Editor: Dr. Muhammad Zahid Iqbal, AIMST University, Malaysia, drmmziqbal@gmail.com UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file: Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.5/10 Reviewers: Rusmir Baljic, Clinic for infectious diseases, Clinical center University of Sarajevo, Bosnia and Herzegovina. rusmir.baljic@gmail.com Dr. Bilge Ahsen KARA, Ankara Gazi Mustafa Kemal Hospital, Turkey, ahsndkyc@gmail.com Prof. Dr. Hassan A.H. Al-Shamahy, Sana'a University, Yemen, shmahe@yemen.net.ye Dr. Jucimary Vieira dos Santos, Hemonorte Dalton Barbosa Cunha, Brazil, jucimaryvieira@yahoo.com.br Dr. Bilge Ahsen KARA, Ankara Gazi Mustafa Kemal Hospital, Turkey, ahsndkyc@gmail.com Similar Articles: PREVALENCE OF HEPATITIS A VIRUS, HEPATITIS B VIRUS, AND HEPATITIS C VIRUS, AMONG PATIENTS WITH HEPATIC JAUNDICE IN SANA’A CITY, YEMEN: A HOSPITAL BASED STUDY SERO-EPIDEMIOLOGICAL STUDY OF HEPATITIS B, C, HIV AND TREPONEMA PALLIDUM AMONG BLOOD DONORS IN HODEIDA CITY- YEMEN EXPLOSION OF HEPATITIS B AND C VIRUSES AMONG HEMODIALYSIS PATIENTS AS A RESULT OF HEMODIALYSIS CRISIS IN YEMEN

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Section: Discussionsupporting

confidence: 86%

Seroprevalence of the Viral Markers of Hepatitis B, Hepatitis C, and Hiv Among Medical Waste Handlers in Some Hospitals in Sana'a City- Yemen

Edrees

Alrahabi

et al. 2022

Univ J Pharm Res

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“…In contrast, our study adopted a laser-induced fluorescence immunochromatographic analyzer, a method we deemed more reliable than ELISA. This assertion is supported by the findings of Navvabi et al (2022), wherein the immunochromatographic test demonstrated high accuracy in diagnosing Hepatitis B surface antigen (HBsAg) in human blood, while the ELISA test exhibited acceptable sensitivity and specificity [43]. Consequently, we posit that our chosen method for CPSE analysis offers enhanced precision.…”

Section: Cpse Valuesmentioning

confidence: 59%

Comparative Evaluation of Diagnostic Methods for Subclinical Benign Prostatic Hyperplasia in Intact Breeding Male Dogs

Laurusevičius,

Šiugždaitė,

Juodžiukynienė

et al. 2024

Animals

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Benign prostatic hyperplasia (BPH) is an androgen-related non-neoplastic enlargement of the prostate gland that commonly affects both reproductive capabilities and the general health of intact dogs. The subclinical form of BPH can be challenging to diagnose due to a lack of clinical signs, even if rectal palpation is performed. Left untreated, this condition poses risks to the dogs’ health and breeding status. This study, involving 65 male dogs, aimed to investigate subclinical BPH through rectal palpation, ultrasonography, and analysis of canine prostatic-specific esterase (CPSE). Of the participants, 35 had subclinical BPH, and 30 served as a healthy control group. Dogs suspected of subclinical BPH, as determined by examination results from ultrasonography and CPSE analysis, underwent fine needle aspiration (FNA) guided by ultrasound to enhance diagnostic precision. Findings revealed distinct differences in rectal palpation and ultrasonography between subclinical BPH and healthy dogs. This study established diagnostic thresholds based on prostatic volume and CPSE values and proposed new thresholds for subclinical BPH. Additionally, results showed that prostate gland volume depended on the weight and the age of the dog. In conclusion, early detection of this condition is possible through various examinations, such as changes in ultrasound features, CPSE levels, and rectal palpation. All together, these methods can aid practitioners in early detection of BPH and assist with scheduling screening programs for dogs, ultimately promoting their overall health and reproductive well-being. In conclusion, we advocate for routine, non-invasive prostate screenings in breeding males, underlining the effectiveness of a combination of various multiple techniques for early subclinical BPH detection.

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“…The commercial immunoassays usually capture HbsAg, having specificity for epitopes present on the antigenic α determinant. The enzyme-linked immunosorbent assay method used in HBsAg detection has a sensitivity and specificity of both about 80%, compared with more than 90% using the immunochromatographic test[ 130 ]. Complete loss of anti-HBc with chronic and high viremic HBV infection after allogeneic stem cell transplantation has been reported[ 131 ].…”

Section: Prevention Of Hepatitis B Reactivationmentioning

confidence: 99%

Prevention and management of hepatitis B virus reactivation in patients with hematological malignancies in the targeted therapy era

Mak,

Law,

Law

et al. 2023

World J Gastroenterol

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Delayed passage of meconium or constipation during the perinatal period is traditionally regarded as a signal to initiate further work up to evaluate for serious diagnoses such as Hirschsprung’s disease (HD), meconium ileus due to Cystic Fibrosis, etc. The diagnosis of HD particularly warrants invasive testing to confirm the diagnosis, such as anorectal manometry or rectal suction biopsy. What if there was another etiology of perinatal constipation, that is far lesser known? Cow’s milk protein allergy (CMPA) is often diagnosed in infants within the first few weeks of life, however, there are studies that show that the CMPA allergen can be passed from mother to an infant in-utero, therefore allowing symptoms to show as early as day one of life. The presentation is more atypical, with perinatal constipation rather than with bloody stools, diarrhea, and vomiting. The diagnosis and management would be avoidance of cow's milk protein within the diet, with results and symptom improvement in patients immediately. Therefore, we discuss whether an alternative pathway to address perinatal constipation should be further discussed and implemented to potentially avoid invasive techniques in patients. This entails first ruling out CMPA with safe, noninvasive techniques with diet modification, and if unsuccessful, then moving forward with further diagnostic modalities.

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Comparative assessment of immunochromatography and ELISA diagnostic tests for HBsAg detection in PCR-confirmed HBV infection

Cited by 6 publications

References 15 publications

Seroprevalence of the Viral Markers of Hepatitis B, Hepatitis C, and Hiv Among Medical Waste Handlers in Some Hospitals in Sana'a City- Yemen

Seroprevalence of the Viral Markers of Hepatitis B, Hepatitis C, and Hiv Among Medical Waste Handlers in Some Hospitals in Sana'a City- Yemen

Comparative Evaluation of Diagnostic Methods for Subclinical Benign Prostatic Hyperplasia in Intact Breeding Male Dogs

Prevention and management of hepatitis B virus reactivation in patients with hematological malignancies in the targeted therapy era

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