Comparative assessment of growth media and incubation conditions for enhanced recovery and isolation of Acinetobacter baumannii from aquatic matrices

Benoit, Thomas G.; Cloutier, Michel; Schop, R.; Lowerison, Mark; Khan, Izhar U. H.

doi:10.1016/j.mimet.2020.106023

Cited by 11 publications

(3 citation statements)

References 38 publications

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“… Acinetobacter species of concern are emerging worldwide, especially in warmer climates, and their prevalence likely will increase with climate change ( 50 – 52 ). River levels of Acinetobacter species can be examined by targeted metagenomic analysis ( 53 ), amplicon assessment ( 54 ), and culture-based methods ( 55 ).…”

Section: Discussionmentioning

confidence: 99%

An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, Including Acinetobacter Tetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent

et al. 2021

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Section: Discussionmentioning

confidence: 99%

An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, Including Acinetobacter Tetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent

et al. 2021

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“…Acinetobacter species of concern are emerging worldwide, especially in warmer climates; and their prevalence likely will increase with climate change (52)(53)(54). River levels of Acinetobacter species can be examined by targeted metagenomic analysis (55), amplicon assessment (56) and culture-based methods (57). Rural rivers have substantial economic and cultural significance for local human communities.…”

Section: Discussionmentioning

confidence: 99%

An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, IncludingAcinetobacterTetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent

Murphy

Barich

Fennessy

et al. 2021

Preprint

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Rivers in rural areas receive continual influx of wastewater carrying antibiotics originally administered to humans and livestock. The entry of antibiotic resistance genes (ARGs) into aquatic systems has been documented for large municipal wastewater treatment plants, but there is less study of the impact of smaller plants that are situated on small rural rivers. We sampled water metagenomes for ARG and taxa composition from the Kokosing State Scenic River, a small rural river in Knox County, Ohio. Samples were obtained upstream, a few meters downstream, and 6 km downstream from the effluent release of the Mount Vernon wastewater treatment plant (WWTP). Through all seasons, the metagenome just downstream of the WWTP effluent showed a substantial elevation of at least 15 different ARGs, including 6 ARGs from Acinetobacter baumannii such as msrE, mphE (macrolide resistance) and tet(39) (tetracycline resistance). The ARGs most prevalent near the effluent pipe persisted 6 km downstream. The taxa distribution near the effluent showed elevation of Acinetobacter species as well as gut-associated taxa, Bacteroides and Firmicutes. The ARG levels and taxa prevalence showed little dependence on seasonal chlorination of effluent. Nitrogen and phosphorus were elevated near the effluent pipe but had no consistent effect on ARG levels. We show that in a rural river microbiome, year-round wastewater effluent substantially elevates ARGs including those of multidrug-resistant A. baumannii.

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“…Given that there is a high diversity of human-associated pathogenic bacteria, protozoa, and viruses that can be transmitted by contaminated water, it is difficult and costly to directly measure and regularly monitor all pathogens in the treated drinking water samples. Therefore, Canada and other countries have developed microbial drinking water quality standards and guidelines for routine monitoring of drinking water using indicators of fecal contamination, namely Escherichia coli and total coliforms [ 3 ]. According to these guidelines, a 100 mL drinking water sample should be free of fecal indicator organisms [ 2 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Quantitative Assessment of First Nations Drinking Water Distribution Systems for Detection and Prevalence of Thermophilic Campylobacter Species

Khan

Murdock

Mahmud

et al. 2022

IJERPH

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Water is considered a major route for transmitting human-associated pathogens. Although microbial water quality indicators are used to test for the presence of waterborne pathogens in drinking water, the two are poorly correlated. The current study investigates the prevalence of thermophilic DNA markers specific for Campylobacter spp. (C. jejuni and C. coli) in source water and throughout the water distribution systems of two First Nations communities in Manitoba, Canada. A total of 220 water samples were collected from various points of the drinking water distribution system (DWDS) between 2016 and 2018. Target Campylobacter spp. were always (100%) detected in a home with a fiberglass (CF) cistern, as well as the community standpipe (SP). The target bacteria were also frequently detected in treated water at the Water Treatment Plant (WTP) (78%), homes with polyethylene (CP) (60%) and concrete (CC) (58%) cisterns, homes with piped (P) water (43%) and water truck (T) samples (20%), with a maximum concentration of 1.9 × 103 cells 100 mL−1 (C. jejuni) and 5.6 × 105 cells 100 mL−1 (C. coli). Similarly, target bacteria were detected in 68% of the source water samples with a maximum concentration of 4.9 × 103 cells 100 mL−1 (C. jejuni) and 8.4 × 105 cells 100 mL−1 (C. coli). Neither target Campylobacter spp. was significantly associated with free and total chlorine concentrations in water. The study results indicate that there is an immediate need to monitor Campylobacter spp. in small communities of Canada and, particularly, to improve the DWDS in First Nations communities to minimize the risk of Campylobacter infection from drinking water sources. Further research is warranted in improving/developing processes and technologies to eliminate microbial contaminants from water.

show abstract

Comparative assessment of growth media and incubation conditions for enhanced recovery and isolation of Acinetobacter baumannii from aquatic matrices

Cited by 11 publications

References 38 publications

An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, Including Acinetobacter Tetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent

An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, Including Acinetobacter Tetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent

An Ohio State Scenic River Shows Elevated Antibiotic Resistance Genes, IncludingAcinetobacterTetracycline and Macrolide Resistance, Downstream of Wastewater Treatment Plant Effluent

Quantitative Assessment of First Nations Drinking Water Distribution Systems for Detection and Prevalence of Thermophilic Campylobacter Species

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