Comparative assessment of faecal diagnostics for detection and predictive modelling of endemic Fasciola hepatica infection in sheep and cattle on Australian farms

George, Sarah; George, Anjitha; Rolfe, P.F.; Emery, D.L.

doi:10.1016/j.vpoa.2018.100001

Cited by 8 publications

(4 citation statements)

References 36 publications

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“…Fecal eggs counts (FEC) are only useful to detect patent infections and have poor sensitivity with low burden infections [ 100 ]. Enzyme-linked immunosorbent assays (ELISAs) such as the coproantigen-ELISAs offer an alternative method of diagnosis from FEC, but they are limited in detecting infection inside the pre-patent period [ 101 , 102 ]. Of the methods mentioned in Table 2, only serological ELISAs were proven to detect specific anti- F. hepatica antibodies as early as ~3 weeks post-infection [ 103 , 104 ]; however, this method cannot distinguish between new and historic infections.…”

Section: Pathogenicity Of Fasciola Spp In Their Definitive Hostsmentioning

confidence: 99%

Pathogenicity and virulence of the liver flukesFasciola hepaticaandFasciolaGiganticathat cause the zoonosis Fasciolosis

et al. 2021

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Fasciolosis caused by the liver flukes Fasciola hepatica and Fasciola gigantica is one of the most important neglected parasitic diseases of humans and animals. The ability of the parasites to infect and multiply in their intermediate snail hosts, and their adaptation to a wide variety of mammalian definitive hosts contribute to their high transmissibility and distribution. Within the mammalian host, the trauma caused by the immature flukes burrowing through the liver parenchyma is associated with most of the pathogenesis. Similarly, the feeding activity and the physical presence of large flukes in the bile ducts can lead to anemia, inflammation, obstruction and cholangitis. The high frequency of non-synonymous polymorphisms found in Fasciola spp. genes allows for adaptation and invasion of a broad range of hosts. This is also facilitated by parasite’s excretory-secretory (ES) molecules that mediate physiological changes that allows their establishment within the host. ES contains cathepsin peptidases that aid parasite invasion by degrading collagen and fibronectin. In the bile ducts, cathepsin-L is critical to hemoglobin digestion during feeding activities. Other molecules (peroxiredoxin, cathepsin-L and Kunitz-type inhibitor) stimulate a strong immune response polarized toward a Treg/Th2 phenotype that favors fluke’s survival. Helminth defense molecule, fatty acid binding proteins, Fasciola -specific glycans and miRNAs modulate host pro-inflammatory responses, while antioxidant scavenger enzymes work in an orchestrated way to deter host oxidant-mediated damage. Combining these strategies Fasciola spp. survive for decades within their mammalian host, where they reproduce and spread to become one of the most widespread zoonotic worm parasites in the world.

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Section: Pathogenicity Of Fasciola Spp In Their Definitive Hostsmentioning

confidence: 99%

Pathogenicity and virulence of the liver flukesFasciola hepaticaandFasciolaGiganticathat cause the zoonosis Fasciolosis

et al. 2021

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“…Th e fascioliasis can be diagnosed using the classical methods of helminthological dissection (Skrjabin, 1928) scatological methods (Esteban et al, 1998;Cringoli et al, 2010;2017;Al-Mamunet al, 2011;Carneiro et al, 2018;Zárate-Rendón et al, 2019), and enzyme-linked immunosorbent test systems, ELISA (Hillyer, 1999;Akca et al, 2014;Munita et al, 2019). It has been shown that both scatological and ELISA have similar resolution (George et al, 2019), which makes the previously obtained results comparable and allows for eff ective control of the prevalence of fascioliasis. Information on the prevalence of fascioliasis among diff erent host species is highly controversial.…”

Section: Introductionmentioning

confidence: 97%

Prevalence of Fascioliasis in Ruminants of the World — meta-analysis

Кручиненко¹,

Mykhailiutenko²,

Petrenko³

2022

Zoodiversity

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Among the gastrointestinal parasitoses, fascioliasis is one of the most common diseases in ruminants. Fasciola spp. is recorded on five continents of the globe, in more than 50 countries. The parasitizing trematode causes economic losses associated with a decrease in milk yield, body weight, and culling of affected carcasses and organs. In this study, we aimed to quantify the prevalence of fascioliasis among ruminants (cattle, sheep and goats) of the world in terms of the odds ratio according to the Mantel–Haenszel test (M–H). Online databases in English, Russian and Ukrainian languages were searched for publications from January 2002 to September 2020. This meta-analysis included 42 studies with ruminant hosts. Data on the spread of fascioliasis were collected from different continents of the globe: North and South America, Europe, Asia and Africa. The results of the study found that the overall prevalence of fascioliasis in cattle was 6.41 %, while in small ruminants it was only 2.03 %. The disease in cattle was recorded 1.48 times more often than in sheep and goats. Egger’s regression test revealed no significant publication bias (P = 0.265). The results of the meta-analysis confirm that the causative agent of fascioliasis circulates mainly in the emerging countries. The updated data on fascioliasis will expand the screening strategy to maintain the health of farm ruminants and reduce economic losses.

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“…For diagnosis, the farm history and epidemiology data might be followed to suggest the fluke risk; however, traditional examination of slaughtered animals remains the most definitive indicator. Also, identification of fluke eggs in fecal samples of clinically suspected animals is useful; however, it's time-consuming, laborious and lack of sensitivity, particularly, in animals having low parasitic burdens [9,10]. In last decade, serological assays such as enzyme-linked immunosorbent assay (ELISA) offers a rapid, valuable and sensitive tool for indication of infection based on positive reaction of anti-fluke antibodies in blood samples [11].…”

Section: Introductionmentioning

confidence: 99%

Serological Prevalence of Anti-Fasciola Hepatica Antibodies in Sheep

Gharban,

Sray,

Essa

2024

Egyptian Journal of Veterinary Sciences

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HIS STUDY aims to investigate serological prevalence of F. hepatica in sheep with estimating the degree of positivity and association to epidemiological risk factors. Totally, 460 sheep were selected and blood sampled to testing the obtained sera by indirect ELISA. The findings revealed that the prevalence of anti-F. hepatica IgG antibodies in sheep was 39.78% with existence 1.96% of dubious cases. According to degree of seropositivity, heavy infestation (49.73%) was increased significantly in comparison with mild (13.11%) and moderate (37.16%) infestations. The results of risk factors showed a significant increasing in positivity, Odds ratio and relative risk in sheep of 13-24 months old; and reduction in sheep of 6 months old. Subsequently, mild infestation was increased at 6 months old; whereas, moderates and heavies were appeared at 25 and 13-24 months old, respectively. Seropositivity, Odds ratio and relative risk were higher in Al-Kut than Al-Hay and Al-Numaniyah. Concerning degrees of positivity, mild and moderate infestations were detected at Al-Hay; while, heavy infestation in Al-Numaniyah. No significant variation was found between females and males; however, females appeared at higher risk than males. For degrees of seropositivity, moderates and heavies were elevated significantly in males and females, respectively. In conclusion, this represents the first serological study targets detection of F. hepatica in sheep of Wasit province (Iraq). Therefore, additional investigations using the advanced assays (serology and molecular assay) appear necessary to determine the rate of positivity in animals, and areas at higher risk of parasitic contamination and infection.

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Comparative assessment of faecal diagnostics for detection and predictive modelling of endemic Fasciola hepatica infection in sheep and cattle on Australian farms

Abstract: Graphical abstract

Cited by 8 publications

References 36 publications

Pathogenicity and virulence of the liver flukesFasciola hepaticaandFasciolaGiganticathat cause the zoonosis Fasciolosis

Pathogenicity and virulence of the liver flukesFasciola hepaticaandFasciolaGiganticathat cause the zoonosis Fasciolosis

Prevalence of Fascioliasis in Ruminants of the World — meta-analysis

Serological Prevalence of Anti-Fasciola Hepatica Antibodies in Sheep

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