Comparative architecture of silks, fibrous proteins and their encoding genes in insects and spiders

Craig, Catherine L.; Riekel, Christian

doi:10.1016/s1096-4959(02)00095-7

Cited by 130 publications

(88 citation statements)

References 44 publications

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“…The H-chain is by far the major component of silk (*75% (w/w) of the cocoon) and is responsible for the chemico-physical properties and mechanic properties of silk fiber (Craig and Riekel 2002). H-chain is encoded by the fibroin-H (Fib-H) gene, which is *16,790 bp long and has two exons and one intron (Zhou et al 2000).…”

Section: Discussionmentioning

confidence: 99%

New and highly efficient expression systems for expressing selectively foreign protein in the silk glands of transgenic silkworm

Zhao

Zhang

et al. 2009

Transgenic Res

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We constructed three different fibroin H-chain expression systems to estimate the efficacy of producing recombinant proteins in the cocoon of transgenic silkworms. The results showed that the three different EGFP/H-chain fusion genes were all expressed selectively in the posterior silk gland of the transgenic silkworm. The recombinant protein content of transgenic silkworm cocoons is up to 15% (w/w) when using the most highly efficient H-chain expression system. To our knowledge, in comparison with silkworm silk gland expression systems in the literature, the highly efficient expression system developed in this study is the most efficient silkworm silk gland expression system to date. This expression system is the best candidate for foreign gene production and for creation of novel functional silk material. The results suggested the N-terminal domain and the intron of the H-chain gene are important in the secretion of fibroin and its transcription, respectively.

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Section: Discussionmentioning

confidence: 99%

New and highly efficient expression systems for expressing selectively foreign protein in the silk glands of transgenic silkworm

Zhao

Zhang

et al. 2009

Transgenic Res

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“…A key element in known sequences are the crystalline poly-alanine and the less crystalline glycine-rich motifs. Even though it is accepted that the silk protein secondary structure is a function of interplay between these regions (Craig and Riekel, 2002), the link to mechanical function is still far from clear. Parts of the sequences have a higher probability to form alpha-helices whereas others are more prone to establish beta-sheets.…”

Section: Secondary Structure Of Silk Proteinmentioning

confidence: 99%

Spider Silk Proteins – Mechanical Property and Gene Sequence

et al. 2005

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-Spiders spin up to seven different types of silk and each type possesses different mechanical properties. The reports on base sequences of spider silk protein genes have gained importance as the mechanical properties of silk fibers have been revealed. This review aims to link recent molecular data, often translated into amino acid sequences and predicted three dimensional structural motifs, to known mechanical properties.

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“…1,2) The sericin of Bombyx mori (the domesticated silkworm) has been studied extensively in connection with the industrial use of B. mori silk. 3) Sericin accounts for 20-30 wt% of B. mori cocoon fiber, and is usually removed by degumming during silk processing to improve the luster and texture of the silk.…”

mentioning

confidence: 99%

Preparation of Gel Film fromBombyx moriSilk Sericin and Its Characterization as a Wound Dressing

Teramoto

Kameda

Tamada

2008

Bioscience, Biotechnology, and Biochemistry

102

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Comparative architecture of silks, fibrous proteins and their encoding genes in insects and spiders

Cited by 130 publications

References 44 publications

New and highly efficient expression systems for expressing selectively foreign protein in the silk glands of transgenic silkworm

New and highly efficient expression systems for expressing selectively foreign protein in the silk glands of transgenic silkworm

Spider Silk Proteins – Mechanical Property and Gene Sequence

Preparation of Gel Film fromBombyx moriSilk Sericin and Its Characterization as a Wound Dressing

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