Comparative antioxidant activity and HPLC profiles of some selected Korean thistles

Abstract

As yet, no comparative analyses have been conducted regarding the comparative antioxidant activities and HPLC profiles of thistles distributed in Korea. Thus, this study was performed in order to evaluate the antioxidant potentials of seven Korean thistles: Cirsium lineare, Cirsium chanroenicum, Cirsium setidens, Cirsium japonicum var. ussuriense, Cirsium nipponicum, Cirslum pendulum and Carduus crispus, via peroxynitrite and DPPH free radical assays. Among seven Korean thistles, Carduus crispus exhibited the most significant antioxidant activity in both DPPH assay and peroxynitrite. In order to characterize the compounds contained in Korean thistles, we conducted HPLC analyses on the following ten flavonoids: luteolin-5-glucoside (1), luteolin-7-glucoside (2), apigenin-7-glucoside (3), hispidulin-7-neohesperidoside (4), apigenin-7-glucuronide (5), cirsimarin (6), pectolinarin (7), luteolin (8), apigenin (9) and acacetin (10). The results of our HPLC analyses indicated the presence of pectolinarin in the whole plants of C. setidens, C. lineare, C. nipponicum, C. pendulum, the aerial and underground parts of C. japonicum var. ussuriense, and the aerial parts of C. chanroenicum. Moreover, we were able to identify hispidulin-7-neohesperidoside and luteolin-7-glucoside in the whole plants of Carduus crispus, acacetin in the aerial parts of C. chanroenicum, cirsimarin in C. lineare.

Polyacetylene Compound from Cirsium japonicum var. ussuriense Inhibits the LPS-Induced Inflammatory Reaction via Suppression of NF-κB Activity in RAW 264.7 Cells

“…Linarin, luteolin, acacetin etc. from the Compositae family has been reported to have various biological activities including anti-oxidant activity (Park et al, 2004;Jeong et al, 2008). However, no report has been issued on anti-infl ammatory activity or mode of action by 1-Heptadecene-11, 13-diyne-8, 9, 10-triol (PA) from the root of Cirsium japonicum var.…”

Determination of pectolinarin inCirsiumspp. using HPLC/UV analysis

“…Cirsium spp. is also bioactive and has antimicrobial, anti-diabetic, anti-oxidant, hepatoprotective, antiinflammatory, vasorelaxant, and anti-cancer properties (Perez et al 2001;Park et al 2004;Nazaruk and Jakoniuk 2005;Liu et al 2007;Jeong et al 2008;Kim et al 2008;Ku et al 2008;Lim et al 2008;Yoo et al 2008).…”

“…contains various phytochemicals, including flavonoids, phenolic acids, lignans, polyacetylenes, acetylenes, sterols, triterpenes, susquiterpene lactones, and alkaloids (Lee et al 1994;JordonThanden and Louda 2001;Chung et al 2002;Yim et al 2003;Jeong et al 2008). The flavonoids include compounds such as pectolinarin, luteolin, linarin, hispidulin-7-neohesperidoside, pectolinarigenin, apigenin, quercetin, luteolin 5-O-glucoside, and 5,7-dihydroxy-6,4-dimethoxy flavones (Ganzera et al 2005;Liu et al 2006;Jung et al, 2012;Sun et al 2012).…”

The HPLC Fingerprint Analysis of SelectedCirsiumSpecies with Aid of Chemometrics

“…8 High-performance liquid chromatography (HPLC) fingerprint technique, accepted by World Health Organization (WHO), is widely used for quality control of plant raw material 9 and this method was also used for the study of some Cirsium species. [10][11][12][13][14] The chemometric methods, as the application of mathematical and statistical techniques, can be important instruments to retrieve more information from the chromatographic data and for evaluating of similarity between various herbal species. 15 In our work, the fingerprint analysis of twelve thistles (ten known and two unknown) were performed using HPLC gradient elution technique.…”