Comparative antibacterial potential of selected aldehyde-based biocides and surfactants against planktonic Pseudomonas fluorescens

Simões, Manuel; Pereira, Maria Olívia; Machado, Idalina; Simões, Lúcia C.; Vieira, M. J.

doi:10.1007/s10295-006-0120-5

Cited by 53 publications

(56 citation statements)

References 39 publications

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“…An additional common problem in susceptibility assays is the presence of bovine serum albumin in liquid medium. Serum albumin quenches biocidal activities of disinfectants, thereby impairing the efficacy of biocidal formulations markedly (11). In our experiments, we also found this reported quenching of biocidal actives by organic soiling for both test formulations, indicated by the prolonged contact times necessary to achieve complete killing of mycobacteria in the presence of a high organic load.…”

Section: Discussionsupporting

confidence: 83%

Rapid Evaluation of the Mycobactericidal Efficacy of Disinfectants in the Quantitative Carrier Test EN 14563 by Using Fluorescent Mycobacterium terrae

Steinhauer

Eschenbacher

Radischat³

et al. 2010

Appl Environ Microbiol

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To prevent transmission of mycobacterial pathogens, medical devices must be disinfected by germicides with proven mycobactericidal activity. The quantitative carrier test EN 14563 provides an international standard for evaluation of the mycobactericidal activity of disinfectants under practical conditions. However, tests according to the EN 14563 standard are based on cultivation, and results are available only after 21 days. The aim of this study was to accelerate assessment of dosage and contact times of mycobactericidal preparations based on the EN 14563 standard. To this end, a gfp gene was constructed with a codon usage adapted for Mycobacterium tuberculosis. Expression of the gfp m 2؉ gene in Mycobacterium terrae improved the detection sensitivity by 10-fold over that with a previously used reporter strain. Peracetic acid and a cation-active formulation were tested as commercially available disinfectants for medical devices. M. terrae expressing gfp m 2؉ was used to determine dosage and contact times for the two test germicides. Fluorescence measurements correlated well with growth of the reporter strain, demonstrating that the fluorescence reliably indicated the number of viable cells. The fluorescence enabled us to determine the mycobactericidal efficacy of the test disinfectants according to the quantitative carrier test EN 14563 standard within at least 15 days. In conclusion, this study establishes gfp m 2؉ -expressing M. terrae as a new reporter strain for reliable evaluation of mycobactericidal activities of disinfectants with a superior sensitivity and in a significantly shorter time than previously possible.Prevention of infections by mycobacteria is an important goal due to their paramount clinical relevance. This task presents a significant challenge, since mycobacteria are intrinsically resistant to many chemical agents (2). In order to avoid device-related transmission of mycobacterial pathogens, germicides developed for disinfection of medical devices must be proven to possess mycobactericidal activity. Conventional testing of disinfectants for their tuberculocidal activity is based on cultivation techniques. Mycobacterium terrae, which has been demonstrated to possess resistance to chemical disinfectants similar to that of Mycobacterium tuberculosis (5), is used as a surrogate for establishing tuberculocidal activity of germicides. However, such an assay takes at least 3 weeks because of the slow growth of M. terrae. In order to accelerate the process of screening chemicals for mycobactericidal activity, Zafer et al. has described the use of gfp-expressing M. terrae in quantitative suspension tests (18). Generally, suspension tests are used to determine the overall efficacy of disinfectants against mycobacteria. However, mycobacteria are used in cell suspensions in these experiments and thus are likely to be more accessible to disinfectants. Therefore, it is essential to demonstrate the mycobactericidal efficacy of disinfectants for medical devices under practical conditions. For these reaso...

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Section: Discussionsupporting

confidence: 83%

Rapid Evaluation of the Mycobactericidal Efficacy of Disinfectants in the Quantitative Carrier Test EN 14563 by Using Fluorescent Mycobacterium terrae

Steinhauer

Eschenbacher

Radischat³

et al. 2010

Appl Environ Microbiol

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“…Bacterial growth conditions were 27± 2 o C and pH 7, with glucose as the main carbon source. Both bacteria were allowed to grow in a sterile synthetic nutrient medium consisting of 5 g l -1 glucose, 2.5 g l -1 peptone, and 1.25 g l -1 yeast extract, in 0.2 M phosphate buffer (PB) at pH 7 (Simões et al, 2006). Chromobacterium violaceum CV12472 was also used to determine QSI.…”

Section: Bacteria and Culture Conditionsmentioning

confidence: 99%

The effects of ferulic and salicylic acids on Bacillus cereus and Pseudomonas fluorescens single- and dual-species biofilms

Lemos

Borges

Teodósio

et al. 2014

International Biodeterioration & Biodegradation

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Biofilms are a problem to food industries, causing equipment damage, increased energy costs, and food spoilage, and they are a potential harbour of pathogenic microorganisms. Their extreme anti-microbial resistance means that novel control strategies are necessary. Plant secondary metabolites (phyto-chemicals) have demonstrated promising antimicrobial properties when applied against planktonic cells and biofilms. The aim of this study was to test the effectiveness of two phenolic acids: ferulic (FA) and salicylic (SA), alone and in combination (FSA) on the prevention and control of Bacillus cereus and Pseudomonas fluorescens biofilms. Additional tests were performed to ascertain the effects of FA and SA on bacterial motility, surface properties (physicochemical properties and surface charge), and quorum sensing inhibition (QSI). The effects of a concentration two times the minimum inhibitory concentration (500 g mL -1 ) were assessed on single-and dual-species biofilms. The results demonstrated that only swimming was affected by FA and SA and no clear relationship was obtained between the effects of phenolic acids on motility and biofilm prevention. The bacterial physico-chemical surface properties and charge were affected by the phenolic acids. Salicylic acid demonstrated capacity for QSI. However, both bacteria were able to form single-and dual-species biofilms in the presence of the phenolic acids. The application of FA and SA (single and combined) to biofilms caused low to moderate inactivation and removal. However, dual-species biofilms formed in the presence of phenolic acids were highly susceptible to a second exposure to the chemicals. The continuous exposure of dual-species biofilms to the phenolic acids decreased their resilience and resistance to inactivation and removal. This study clarifies the role of FA and SA in the prevention and control of biofilms formed by two important food spoilage bacteria.2

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“…34,35 No critical micelle concentration can be defined for BSA because it is not an amphiphilic surfactant. However, an apparent critical micelle concentration of ∼0.076 μM is reported in the literature.…”

Section: Kandadai Et Al Articlementioning

confidence: 99%

Oxygenation-Enhanced Radiation Therapy of Breast Tumors

Skliar¹

2011

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. REPORT DATE (DD-MM-YYYY)2. REPORT TYPE 3. DATES COVERED (From -To) 4. TITLE AND SUBTITLE 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e. TASK NUMBER E-Mail:5f. WORK UNIT NUMBER The treatment of locoregional breast cancer has evolved from radical mastectomy to targeted local therapy with breast conservation. The efficacy of conserving treatments of breast cancers is impeded by tumor hypoxia, which affects 50% of locally advanced breast tumors. Poor oxygenation of hypoxic tumors reduces their sensitivity to X-ray and γ-radiation, which requires a high radiation dose for efficacy compared to normally oxygenated tissues. This Concept project provides initial investigation the method for targeted oxygen release from oxygen carriers as a step towards as a novel strategy of radio sensitization of hypoxic breast tumors, and studies the effect of different targeting mechanisms on the oxygen mass transfer. Specific results include the development of stabilized perfluorocarbon emulsions as oxygen carriers, their characterization, and response to external triggers, including elevated temperature, reduced pressure, and dwell time. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION REPORT NUMBER SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR'S ACRONYM(S) U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012 SPONSOR/MONITOR'S REPORT NUMBER(S) DISTRIBUTION / AVAILABILITY STATEMENT IntroductionThe treatment of locoregional breast cancer has evolved from radical mastectomy to targeted local therapy with breast conservation. The efficacy of conserving treatments of breast cancers is impeded by tumor hypoxia. It has been reported [1] that close to 50% of locally advanced breast cancers exhibit hypoxic or even anoxic regions within a tumor mass. Poor oxygenation of hypoxic tumors reduces their sensitivity to X-ray and γ-radiation, which requires a high radiation dose for efficacy compared to normally oxygenated tissues [2]. This increase is approximately 3-fold in the case of X-ray therapy [3], which ...

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Comparative antibacterial potential of selected aldehyde-based biocides and surfactants against planktonic Pseudomonas fluorescens

Cited by 53 publications

References 39 publications

Rapid Evaluation of the Mycobactericidal Efficacy of Disinfectants in the Quantitative Carrier Test EN 14563 by Using Fluorescent Mycobacterium terrae

Rapid Evaluation of the Mycobactericidal Efficacy of Disinfectants in the Quantitative Carrier Test EN 14563 by Using Fluorescent Mycobacterium terrae

The effects of ferulic and salicylic acids on Bacillus cereus and Pseudomonas fluorescens single- and dual-species biofilms

Oxygenation-Enhanced Radiation Therapy of Breast Tumors

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