Comparative anti-osteoporotic properties of the leaves and roots of Marantodes pumilum var. alata in postmenopausal rat model

Abstract

Background Marantodes pumilum var. alata ( MPva ), popularly known as Kacip Fatimah, is widely used to maintain female reproductive health, facilitate post-partum recovery and manage symptoms of menopause and osteoporosis in South-East Asia. This study aims to further evaluate the osteoprotective potential of MPva in view of reports of its bone-protective properties in postmenopausal condition. Methods Thirty female Sprague-Dawley rats were sorted into 5 groups (n = 6) namely: MPv (leaf treatment); MPr (root treatment); ERT (estrogen treatment); OVXC (untreated ovariectomized control) and Sham (untreated sham-operated control). All rats (except the Sham) were ovariectomized to induce a state of estrogen deficiency that simulates menopause. Two weeks after ovariectomy, the rats were treated for 8 weeks with oral gavages of estrogen and plant extracts. The ERT group received 64.5 μg/kg/day dose of estrogen while MPv and MPr groups received 20 mg/kg/day dose of leaf and root extracts , respectively. At the end of treatment, left femora were excised from euthanized rats and investigated for changes in bone micro-architecture, mineral density, and biomechanical properties. Results Bone volume fraction, degree of anisotropy and structure-model-index of bone were significantly improved ( p < 0.05) in the MPv group compared to OVXC. Breaking force and maximum stress of bone were also significantly higher ( p < 0.05) in the MPv group compared to the OVXC. Conclusion Treatment with MPva leaf protected bone microarchitecture and density against osteoporosis-related changes in postmenopausal rats. Similar to estrogen, the protective effects of MPva leaf translated into better-enhanced bone mechanical properties compared to the root treatment.

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“…In the United States alone, around 44 million people aged above 50 years suffer from osteoporosis, a systemic disease which may lead to fractures and require multiple surgeries. [1][2][3] Therefore, the demand for biomaterials that can be suggested to enhance tissue regeneration/rehabilitation after traumas, tumors, osteoporosis, and damage has been enlarged recently. [4][5][6] This requires that both mechanical and biological behavior of such biomaterials must be improved to meet the requested properties of host environment.…”

Peperomia pellucida extracts stimulates bone healing in alveolar socket following tooth extraction

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Authentication of Marantodes pumilum (Blume) Kuntze: A Systematic Review

“…Researchers have used hyphenated chromatographic and mass spectrometric techniques, such as liquid chromatography-mass spectrometry (LC/MS), gas chromatography-mass spectrometry (GC/MS), and capillary electrophoresis-mass spectrometry (CE/MS), to authenticate plant materials. In this systematic review study, LC/MS ( Giaze et al, 2019 ), LC/MS/MS ( Dharmani et al, 2019 ), UPLC/MS/MS ( Wu et al, 2019 ), and LC/ESI/TOF ( Avula et al, 2011 ) were used to determine phytochemical compositions of different M. pumilum plant parts, whereas UPLC-ESI/MS/MS was used to detect components between M. pumilum var. alata and var.…”