Comparative and functional genomics reveals genetic diversity and determinants of host specificity among reference strains and a large collection of Chinese isolates of the phytopathogen Xanthomonas campestris pv. campestris

He, Yanan; Zhang, Liang; Jiang, Bo-Le; Zheng-chun, Zhang; Xu, Ruihua; Tang, Dongjie; Qin, Jing; Jiang, Wei; Zhang, Xiaxia; Liao, Jie; Cao, Jin-Ru; Zhang, Suisheng; Mei-liang, Wei; Liang, Xiaoyan; Lu, Guang‐Tao; Feng, Jia‐Xun; Chen, Baoshan; Cheng, Jing

doi:10.1186/gb-2007-8-10-r218

Cited by 84 publications

(91 citation statements)

References 86 publications

(148 reference statements)

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“…Moreover, a previous study demonstrated the direct binding of Clp to promoter regions [26]. The data from the present study indicate that eglXoA is regulated by ClpXo, which is consistent with previous Xcc studies that show that Clp is involved in the expression of extracellular enzymes of the T2SS [24,26]. Experimental evidence was provided by EMSA, wherein the ClpXo-eglXoA promoter region complex showed gel retardation, indicating that ClpXo directly binds to the eglXoA promoter region.…”

Section: Discussionsupporting

confidence: 80%

“…Clp is a homologue (45% amino acid sequence identity) of the model transcription factor CRP of E. coli. Clp in Xcc also influences the expression of a number of genes, especially the genes in the T2SS [23,24]. The clp gene in Xoo was isolated and characterized [34], and a mutation in clpXo resulted in a significant decrease in the production of cellulase, xylanase, and EPS.…”

Section: Discussionmentioning

confidence: 99%

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Expressional Regulation of the Virulence Gene eglXoA Encoding Endoglucanase, Dependent on HrpXo and Cyclic AMP Receptor-Like Protein (Clp) in Xanthomonas oryzae pv. oryza

Temuujin¹,

Hw²

2017

J Plant Pathol Microbiol

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Expressional Regulation of the Virulence Gene eglXoA Encoding Endoglucanase, Dependent on HrpXo and Cyclic AMP Receptor-Like Protein (Clp) in Xanthomonas oryzae pv. oryza

Temuujin¹,

Hw²

2017

J Plant Pathol Microbiol

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“…In addition, our previous array-based comparative genome hybridization analyses demonstrated that the ORF encoding ADK Xcc is highly conserved in all of the 18 other X. campestris pv. campestris strains tested (25). These results suggest that the ADK enzyme is widely distributed among X. campestris pv.…”

Section: Discussionmentioning

confidence: 59%

An Adenosine Kinase Exists inXanthomonas campestrisPathovar campestris and Is Involved in Extracellular Polysaccharide Production, Cell Motility, and Virulence

Yong-qin

et al. 2009

J Bacteriol

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Adenosine kinase (ADK) is a purine salvage enzyme and a typical housekeeping enzyme in eukaryotes which catalyzes the phosphorylation of adenosine to form AMP. Since prokaryotes synthesize purines de novo and no endogenous ADK activity is detectable in Escherichia coli, ADK has long been considered to be rare in bacteria. To date, only two prokaryotes, both of which are gram-positive bacteria, have been reported to contain ADK. Here we report that the gram-negative bacterium Xanthomonas campestris pathovar campestris, the causal agent of black rot of crucifers, possesses a gene (designated adk Xcc ) encoding an ADK (named ADK Xcc ), and we demonstrate genetically that the ADK Xcc is involved in extracellular polysaccharide (EPS) production, cell motility, and pathogenicity of X. campestris pv. campestris. adk Xcc was overexpressed as a His 6 -tagged protein in E. coli, and the purified His 6 -tagged protein exhibited ADK activity. Mutation of adk Xcc did not affect bacterial growth in rich and minimal media but led to an accumulation of intracellular adenosine and diminutions of intracellular ADK activity and ATP level, as well as EPS. The adk Xcc mutant displayed significant reductions in bacterial growth and virulence in the host plant.

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“…A complemented strain named GXC2088 was also constructed by introducing the recombinant plasmid pLAFRJxrvA, which carries the wild-type xrvA gene (Table 1), into the mutant GXN1280 (see Methods for details). Our previous aCGH (array-based comparative genome hybridization) analyses showed that the pLAFR6 derivative has only one copy in Xcc cells (He et al, 2007). Since plasmid pLAFRJxrvA and pLAFR6 derivatives contain the same replication origin, and Xcc and Xoo are closely related, it is most likely that the complemented strain GXC2088 harbours a single copy of plasmid pLAFRJxrvA and thus possesses only one functional copy of the xrvA gene.…”

Section: Xrva Is Required For the Virulence And Hr Elicitation Of Xoomentioning

confidence: 99%

The xrvA gene of Xanthomonas oryzae pv. oryzae, encoding an H-NS-like protein, regulates virulence in rice

et al. 2009

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Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight disease in rice, one of the most serious rice diseases. The xrvA gene from Xoo strain 13751 encodes a protein containing a histone-like nucleoid-structuring protein (H-NS) domain. The expression of xrvA in strain 13751 was enhanced in XOM2 minimal medium. Mutation of the xrvA gene of strain 13751 led to a significant reduction in virulence in the host plant rice, a delayed hypersensitive response in the nonhost castor-oil plant, a decrease in extracellular polysaccharide and diffusible signal factor production, and an increase in intracellular glycogen accumulation. Northern hybridization analyses revealed that the virulence-associated genes hrpG, hrpX, rpfC, rpfF, rpfG and gumB were downregulated in the xrvA mutant compared to the wild-type and complemented strains. Interestingly, increase of copy number of xrvA in the wild-type strain 13751 resulted in a strain showing similar phenotypes as the xrvA mutant and a reduction of the expression of gumB, hrpX, rpfC, rpfF and rpfG. These findings indicate that the xrvA gene, which is highly conserved in the sequenced strains of Xanthomonas, encodes an important regulatory factor for the virulence of Xoo.

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Comparative and functional genomics reveals genetic diversity and determinants of host specificity among reference strains and a large collection of Chinese isolates of the phytopathogen Xanthomonas campestris pv. campestris

Cited by 84 publications

References 86 publications

Expressional Regulation of the Virulence Gene eglXoA Encoding Endoglucanase, Dependent on HrpXo and Cyclic AMP Receptor-Like Protein (Clp) in Xanthomonas oryzae pv. oryza

Expressional Regulation of the Virulence Gene eglXoA Encoding Endoglucanase, Dependent on HrpXo and Cyclic AMP Receptor-Like Protein (Clp) in Xanthomonas oryzae pv. oryza

An Adenosine Kinase Exists inXanthomonas campestrisPathovar campestris and Is Involved in Extracellular Polysaccharide Production, Cell Motility, and Virulence

The xrvA gene of Xanthomonas oryzae pv. oryzae, encoding an H-NS-like protein, regulates virulence in rice

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