Comparative and functional genomics of lipases in holometabolous insects

Horne, Irene; Haritos, Victoria S.; Oakeshott, John G.

doi:10.1016/j.ibmb.2009.06.002

Cited by 109 publications

(125 citation statements)

References 177 publications

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“…Endopeptidases and exopeptidases [involved in the initial and final steps of protein digestion, respectively] are particularly abundant (28,155,180). There are also 52 and 29 proteins with predicted carbohydrase and lipase activity, respectively (84,85) (Figure 2a). This diverse enzymatic repertoire may be optimized to deal with the nature and complexity of the diet of Drosophila, which is rich in decaying fruits.…”

Section: Digestive Enzymesmentioning

confidence: 99%

The Digestive Tract of Drosophila melanogaster

2013

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The digestive tract plays a central role in the digestion and absorption of nutrients. Far from being a passive tube, it provides the first line of defense against pathogens and maintains energy homeostasis by exchanging neuronal and endocrine signals with other organs. Historically neglected, the gut of the fruit fly Drosophila melanogaster has recently come to the forefront of Drosophila research. Areas as diverse as stem cell biology, neurobiology, metabolism, and immunity are benefitting from the ability to study the genetics of development, growth regulation, and physiology in the same organ. In this review, we summarize our knowledge of the Drosophila digestive tract, with an emphasis on the adult midgut and its functional underpinnings. 377

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Section: Digestive Enzymesmentioning

confidence: 99%

The Digestive Tract of Drosophila melanogaster

2013

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“…Digestive proteases played two critical roles in an insect's physiology: breaking down proteins into amino acids essential for growth and development, and inactivating ingested protein toxins [3032]. Lipases were fundamental to many physiological processes underpinning insect reproduction, development, defense from pathogens and oxidative stress, and pheromone signaling [31,33]. In this study, significantly higher trypsin levels were observed in nymphs grown in elevated CO 2 compared with ambient CO 2 levels, however, there was no significant difference in lipase levels between the two treatments.…”

Section: Nilaparvata Lugens Chemical Composition and Enzyme Activitiesmentioning

confidence: 99%

Effects of elevated CO2 on the nutrient compositions and enzymes activities of Nilaparvata lugens nymphs fed on rice plants

Zeng

Huang

Су

et al. 2012

Sci. China Life Sci.

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Elevated CO 2 may reduce the tolerance of Nilaparvata lugen (N. lugens) to adverse environmental factors through the biological and physiological degeneration of N. lugens. In an artificial climate box, under 375 and 750 μL L 1 CO 2 levels, the rice stems nutrient content, the nutrient content and enzyme activities of N. lugens nymph fed on rice seedlings exposed to ambient and elevated CO 2 were studied. The results showed that rice stems had significantly higher protein and total amino acid levels under ambient than elevated CO 2 levels. Nymphs had significantly higher protein levels in the ambient CO 2 treatment, while their glucose levels were significantly lower under ambient CO 2 conditions. Significantly higher trypsin activity was observed in nymphs grown in elevated CO 2 . Significantly lower activities of the protective enzymes total superoxide dismutase and catalase were observed in the nymphs under ambient CO 2 . Meanwhile, the activity of the detoxification enzyme glutathione S-transferase was significantly higher in the ambient CO 2 treatment. Measuring how energy and resources were allocated to enzymes in N. lugens nymphs under elevated CO 2 conditions can provide a more meaningful evaluation of their metabolic tolerances to adverse climatic conditions.

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“…Lipases are abundant in insect genomes. The majority of them evolved after divergence of insect orders [178], and thus, they potentially possess properties not present among lipases from other organisms. Insect lipase specificities and enzymatic properties, however, remain poorly studied in general.…”

Section: Applications Of Fa-modifying Enzymesmentioning

confidence: 99%

Biotechnological potential of insect fatty acid-modifying enzymes

Tupec

Buček

Valterová

2017

Zeitschrift Für Naturforschung C

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There are more than one million described insect species. This species richness is reflected in the diversity of insect metabolic processes. In particular, biosynthesis of secondary metabolites, such as defensive compounds and chemical signals, encompasses an extraordinarily wide range of chemicals that are generally unparalleled among natural products from other organisms. Insect genomes, transcriptomes and proteomes thus offer a valuable resource for discovery of novel enzymes with potential for biotechnological applications. Here, we focus on fatty acid (FA) metabolism-related enzymes, notably the fatty acyl desaturases and fatty acyl reductases involved in the biosynthesis of FA-derived pheromones. Research on insect pheromone-biosynthetic enzymes, which exhibit diverse enzymatic properties, has the potential to broaden the understanding of enzyme specificity determinants and contribute to engineering of enzymes with desired properties for biotechnological production of FA derivatives. Additionally, the application of such pheromone-biosynthetic enzymes represents an environmentally friendly and economic alternative to the chemical synthesis of pheromones that are used in insect pest management strategies.

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Comparative and functional genomics of lipases in holometabolous insects

Cited by 109 publications

References 177 publications

The Digestive Tract of Drosophila melanogaster

The Digestive Tract of Drosophila melanogaster

Effects of elevated CO2 on the nutrient compositions and enzymes activities of Nilaparvata lugens nymphs fed on rice plants

Biotechnological potential of insect fatty acid-modifying enzymes

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