Comparative analytical sensitivities of six rapid influenza A antigen detection test kits for detection of influenza A subtypes H1N1, H3N2 and H5N1

Chan, Kwok‐Hung; Lam, S. Y.; Puthavathana, P.; Nguyen, Tung; Long, Hoang Thuy; Pang, Chiu Mei; Chan, Kwok‐Hung; Cheung, Chung Yan; Seto, W. H.; Peiris, Malik

doi:10.1016/j.jcv.2006.11.010

Cited by 60 publications

(42 citation statements)

References 7 publications

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“…It detected the influenza A H3N2 and H1N1 virus culture supernatants at a higher dilution than the other assays, including those with comparable sensitivity for the influenza A H5N1 strains. This is similar to a previous report that found this assay performed somewhat better than five other rapid antigen assays (including assays 6 and 12 evaluated in this study) for detecting various avian and human influenza A viruses [Chan et al, 2007]. The QuickVue Influenza A þ B assay was also one of the six more sensitive assays for detecting influenza B.…”

Section: Discussionsupporting

confidence: 90%

“…One study found that rapid antigen tests detected the current circulating human influenza A H3N2 and H1N1 subtypes with comparable analytic sensitivity, but were less sensitive for influenza A H5N1. This may be due to inadequate or delayed specimen collection, patient age, and lower virus antigen levels in the upper compared to the lower respiratory tract [Chan et al, 2007].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of twenty rapid antigen tests for the detection of human influenza A H5N1, H3N2, H1N1, and B viruses

Taylor

McPhie

Druce

et al. 2009

Journal of Medical Virology

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Twenty rapid antigen assays were compared for their ability to detect influenza using dilutions of virus culture supernatants from human isolates of influenza A H5N1 (clade 1 and 2 strains), H3N2 and H1N1 viruses, and influenza B. There was variation amongst the rapid antigen assays in their ability to detect different influenza viruses. Six of the 12 assays labeled as distinguishing between influenza A and B had comparable analytical sensitivities for detecting both influenza A H5N1 strains, although their ability to detect influenza A H3N2 and H1N1 strains varied. The two assays claiming H5 specificity did not detect either influenza A H5N1 strains, and the two avian influenza-specific assays detected influenza A H5N1, but missed some influenza A H3N2 virus supernatants. Clinical trials of rapid antigen tests for influenza A H5N1 are limited. For use in a pandemic where novel influenza strains are circulating (such as the current novel influenza A H1N1 09 virus), rapid antigen tests should ideally have comparable sensitivity and specificity for the new strains as for co-circulating seasonal influenza strains.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Evaluation of twenty rapid antigen tests for the detection of human influenza A H5N1, H3N2, H1N1, and B viruses

Taylor

McPhie

Druce

et al. 2009

Journal of Medical Virology

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“…Move TruTips to the deep well plates at position 6 column 1, and cycle 1x in Wash Buffer CN-W1. 17. Move TruTips to position 9 column 2 and cycle 1x in Wash CN-W2.…”

Section: Sample Lysis and Reagent Distributionmentioning

confidence: 99%

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

Holmberg

Gindlesperger

Stokes

et al. 2013

JoVE

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TruTip is a simple nucleic acid extraction technology whereby a porous, monolithic binding matrix is inserted into a pipette tip. The geometry of the monolith can be adapted for specific pipette tips ranging in volume from 1.0 to 5.0 ml. The large porosity of the monolith enables viscous or complex samples to readily pass through it with minimal fluidic backpressure. Bi-directional flow maximizes residence time between the monolith and sample, and enables large sample volumes to be processed within a single TruTip. The fundamental steps, irrespective of sample volume or TruTip geometry, include cell lysis, nucleic acid binding to the inner pores of the TruTip monolith, washing away unbound sample components and lysis buffers, and eluting purified and concentrated nucleic acids into an appropriate buffer. The attributes and adaptability of TruTip are demonstrated in three automated clinical sample processing protocols using an Eppendorf epMotion 5070, Hamilton STAR and STARplus liquid handling robots, including RNA isolation from nasopharyngeal aspirate, genomic DNA isolation from whole blood, and fetal DNA extraction and enrichment from large volumes of maternal plasma (respectively).

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“…While diagnostic options are available, some methods have significant limitations. Growth and propagation of novel influenza viruses require biosafety level 3 containment, and the poor sensitivity of rapid influenza diagnostic tests (RIDTs) limits their usefulness for patient management (3,(6)(7)(8)(9). In contrast, nucleic acid amplification tests (NAATs) such as reverse transcription-PCR (RT-PCR) have become the gold standard and a number of commercial assays are available (2,10).…”

mentioning

confidence: 99%

Detection of Influenza H7N9 Virus: All Molecular Tests Are Not Equal

Hatchette

Drews

Bastien³

et al. 2013

J Clin Microbiol

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Comparative analytical sensitivities of six rapid influenza A antigen detection test kits for detection of influenza A subtypes H1N1, H3N2 and H5N1

Cited by 60 publications

References 7 publications

Evaluation of twenty rapid antigen tests for the detection of human influenza A H5N1, H3N2, H1N1, and B viruses

Evaluation of twenty rapid antigen tests for the detection of human influenza A H5N1, H3N2, H1N1, and B viruses

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

Detection of Influenza H7N9 Virus: All Molecular Tests Are Not Equal

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