Comparative Analysis of Yeast Replicative Lifespan in Different Trapping Structures Using an Integrated Microfluidic System

Gao, Zhidong; Xu, Jian; Chen, Kaiyue; Wang, Shujing; Ouyang, Qi; Luo, Chunxiong

doi:10.1002/admt.202000655

Cited by 5 publications

(2 citation statements)

References 38 publications

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“…New tools for RNA tagging and higher resolution microscopy that can offer even more detailed observations of cellular processes continue to be developed. Brighter fluorescent proteins (Lambert et al 2020 ), direct RNA-fluorophore binding like mango and peach RNA aptamers (Cawte et al 2020 ; Kong et al 2021 ), and microfluidics devices (Gao et al 2020 ; Anggraini et al 2022 ), to name just a few, could all provide greater and more specific detail of telomerase dynamics that could never have been done before. For new tools that utilise dyes, in the case of yeast, it requires some optimisation since the yeast cell wall is a great barrier to foreign molecules.…”

Section: Telomerase Rnasmentioning

confidence: 99%

Methods that shaped telomerase research

Bartle,

Wellinger

2023

Biogerontology

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Telomerase, the ribonucleoprotein (RNP) responsible for telomere maintenance, has a complex life. Complex in that it is made of multiple proteins and an RNA, and complex because it undergoes many changes, and passes through different cell compartments. As such, many methods have been developed to discover telomerase components, delve deep into understanding its structure and function and to figure out how telomerase biology ultimately relates to human health and disease. While some old gold-standard methods are still key for determining telomere length and measuring telomerase activity, new technologies are providing promising new ways to gain detailed information that we have never had access to before. Therefore, we thought it timely to briefly review the methods that have revealed information about the telomerase RNP and outline some of the remaining questions that could be answered using new methodology.

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Section: Telomerase Rnasmentioning

confidence: 99%

Methods that shaped telomerase research

Bartle,

Wellinger

2023

Biogerontology

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“…This technique alleviates the need for constant human intervention and permits the use of high-resolution microscopy techniques to monitor the aging process. Despite many research groups developing different devices and employing several distinct strategies, no single device has been universally adopted by the field (comprehensively reviewed by Jo et al and Chen et al; experimentally explored by Gao et al (13–15)).…”

Section: Introductionmentioning

confidence: 99%

The Yeast Lifespan Machine: a microfluidic platform for automated replicative lifespan measurements

Thayer

Robles

et al. 2022

Preprint

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SummaryThe budding yeast, Saccharomyces cerevisiae, has emerged as a model system for studying the aging processes in eukaryotic cells. However, the full complement of tools available in this organism has not been fully applied, in part because of limitations in throughput that restrict the ability to carry out detailed analyses. Recent advances in microfluidics have provided direct longitudinal observation of entire yeast lifespans, but have not yet achieved the normal scale of operation possible in this model system. Here we present a microfluidic platform, called the Yeast Lifespan Machine, where we combine improvements in microfluidics, image acquisition, and image analysis tools to increase robustness and throughput of lifespan measurements in aging yeast cells. We demonstrate the platform’s ability to measure the lifespan of large populations of cells and distinguish long- and short-lived mutants, all with minimal involvement of the experimenter. We also show that environmental pH is capable of significantly modulating lifespan depending on the growth media, highlighting how microfluidic technologies reveal determinants of lifespan that are otherwise difficult to ascertain.

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Recent advances of integrated microfluidic systems for fungal and bacterial analysis

Shi

Wang

Zhang

et al. 2023

TrAC Trends in Analytical Chemistry

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Comparative Analysis of Yeast Replicative Lifespan in Different Trapping Structures Using an Integrated Microfluidic System

Abstract: Aging increases the susceptibility to age-related diseases. [1,2] Research on aging is important in promoting human health. Saccharomyces cerevisiae is the premier model in aging research due to its short generation time and genetic tractability. [3-5]

Cited by 5 publications

References 38 publications

Methods that shaped telomerase research

Methods that shaped telomerase research

The Yeast Lifespan Machine: a microfluidic platform for automated replicative lifespan measurements

Recent advances of integrated microfluidic systems for fungal and bacterial analysis

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