“…They are complicated and almost irreversible for the samples, and the microparticles may have a certain influence on 3D measurements. A method of projecting digital speckle patterns by means of a projector can conveniently create random features on the surface without affecting the samples, and has been applied to many macroscopic fields to achieve 3D measurements of simple shapes [ 34 ], human face [ 35 ], a Venus model [ 36 ], human body [ 37 ], a shape-complex mask [ 38 ], and different types of surfaces [ 39 , 40 ]. Whereas, this speckle projection method is rarely used in the field of microscopy.…”