Comparative analysis of three sperm DNA damage assays and sperm nuclear protein content in couples undergoing assisted reproduction treatment

Lee, Simon; Liu, L.; Murphy, Kristin; Ge, Shao Qin; Hotaling, James M.; Aston, Kenneth I.; Emery, Benjamin R.; Carrell, Douglas T.

doi:10.1093/humrep/deu040

Cited by 113 publications

(95 citation statements)

References 92 publications

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“…However, studies examining the effect of high SDF on assisted conception fertilization, embryo development, pregnancy, miscarriage, and birth outcomes report conflicting results; some studies report a correlation between SDF and outcomes for both in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles (Collins et al, 2008;Robinson et al, 2012), others that IVF but not ICSI outcomes are influenced (Bungum et al, 2007;Zini, 2011;Osman et al, 2015), and others have found no clear association with either assisted fertilization method (Esbert et al, 2011;Ni et al, 2014). These inconsistencies may in part be attributed to study design and the use of different assays, with a recent study reporting that Comet and TUNEL assays were more predictive of clinical outcomes than SCSA (Simon et al, 2014). The use of flow cytometry in SCSA makes standardization difficult because of a degree of user-dependent skill and data interpretation.…”

Section: Introductionmentioning

confidence: 99%

Intervention improves assisted conception intracytoplasmic sperm injection outcomes for patients with high levels of sperm DNA fragmentation: a retrospective analysis

Bradley¹,

McArthur²,

Gee³

et al. 2016

Andrology

104

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SUMMARYSperm DNA fragmentation (SDF) is used in assisted reproductive technology (ART) programs as an indicator for sperm quality, although there is still a lack of consensus as to its clinical utility. In this retrospective study, we examined intracytoplasmic sperm injection (ICSI) outcomes of 1924 infertile patients who underwent SDF analysis using the sperm chromatin integrity test. ART patients were classified as having low [DNA fragmentation index (DFI) <29%] or high SDF (DFI ≥29%) and by whether or not an intervention [physiological intracytoplasmic sperm injection (PICSI), intracytoplasmic morphologically selected sperm injection (IMSI), testicular sperm extraction (TESE)/testicular sperm aspiration (TESA), frequent ejaculation] was performed. High SDF patients who did not have an intervention had a lower fertilization rate and poorer clinical outcomes from blastocyst transfers as compared with low SDF patients; the fertilization rate was 66.0% vs. 70.2% (p = 0.042), single embryo transfer (SET) fetal heart pregnancy rate was 28.5% vs. 45.2% (p = 0.042), and SET live birth rate was 24.9% vs. 40.6% (p = 0.060), respectively. Furthermore, high SDF patients who had an intervention had significantly improved blastocyst transfer outcomes, similar to those of low SDF patients; the SET live birth rate for high SDF intervention patients was 43.8% as compared with 24.9% for high SDF no intervention patients (p = 0.037) and 40.6% for low SDF patients (p = 0.446). Analysis of the three main intervention subgroups for high SDF patients revealed that TESE/TESA patients had the highest SET live birth rate; in comparison with 24.2% for high SDF patients who did not have an intervention, PICSI patients had 38.3% (p = 0.151), IMSI patients had 28.7% (p = 0.680), and TESE/TESA patients had 49.8% (p = 0.020). Our data suggest that SDF results indicate ICSI outcomes and that patients who have high SDF benefit from an intervention.

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Section: Introductionmentioning

confidence: 99%

Intervention improves assisted conception intracytoplasmic sperm injection outcomes for patients with high levels of sperm DNA fragmentation: a retrospective analysis

Bradley¹,

McArthur²,

Gee³

et al. 2016

Andrology

104

View full text Add to dashboard Cite

show abstract

“…Another reason for reduced expression of PLCζ could be related to oxidative stress. DNA and RNA are common targets of reactive oxygen species [37] which might have a critical effect on embryo quality and pregnancy rates in IVF/ICSI treatments [38][39][40] and increased upon heat stress or in varicocele condition [30,41].…”

Section: Discussionmentioning

confidence: 99%

An association between sperm PLCζ levels and varicocele?

Janghorban-Laricheh

Ghazavi-Khorasgani

Tavalaee

et al. 2016

J Assist Reprod Genet

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Purpose We aimed to compare the expression of phospholipase C ζ (PLCζ), as one of the main sperm factors involved in oocyte activation, at both RNA and protein levels in fertile men and those with varicocele. Methods This study included 35 individuals with male factor infertility presenting primary infertility with grade II and III unilateral varicocele and 20 fertile men without varicocele. Semen parameters were assessed according to WHO 2010. Sperm DNA fragmentation, relative expression of PLCζ at messenger RNA, and protein levels were evaluated by sperm chromatin structure assay (SCSA), real-time PCR, and Western blot analysis, respectively. Results The results of this study reveal that the mean relative expression of PLCζ was significantly lower in individuals with varicocele compared to fertile men at both transcription and translation levels. In addition, the percentage of DNA fragmentation was significantly higher in infertile men with varicocele compared to fertile men. Conclusions The findings of the present study illustrate that one of the etiologies of reduced fertility associated with varicocele is the low expression of PLCζ. This effect could subsequently reduce the sperm ability to induce oocyte activation. Therefore, these results hold promise to modify our understanding of reproductive physiology of varicocele state.

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“…Появились публикации о том, что высокий уровень повреждения ДНК связан с нарушением дробления эмбриона, высокой частотой спонтанных абортов и по-вторными потерями беременности после ВРТ [39][40][41][42].…”

Section: Objective Study Of the Correlation Between Abnormal Chromatunclassified

“…В последнее десятилетие опубликованы многочи-сленные исследования, авторы которых пытаются уста-новить корреляцию между фрагментацией ДНК и ста-тусом хроматина [30,[42][43][44][45][46].…”

Section: Objective Study Of the Correlation Between Abnormal Chromatunclassified

“…Отмечено наличие корреляции [42,47,48]. Продемонстрирована и выраженная корреляция между аномальной прота-минизацией хроматина и фрагментацией ДНК, выз-ванной оксидативным стрессом [49].…”

Section: Objective Study Of the Correlation Between Abnormal Chromatunclassified

See 1 more Smart Citation

Abnormal Chromatin Condensation in Spermatozoa and Dna Fragmentation in Spermatozoa: Is There a Correlation?

Брагина¹,

Arifulin²,

Лазарева³

et al. 2017

Androl. genit. hir.

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Comparative analysis of three sperm DNA damage assays and sperm nuclear protein content in couples undergoing assisted reproduction treatment

Abstract: N/A.

Cited by 113 publications

References 92 publications

Intervention improves assisted conception intracytoplasmic sperm injection outcomes for patients with high levels of sperm DNA fragmentation: a retrospective analysis

Intervention improves assisted conception intracytoplasmic sperm injection outcomes for patients with high levels of sperm DNA fragmentation: a retrospective analysis

An association between sperm PLCζ levels and varicocele?

Abnormal Chromatin Condensation in Spermatozoa and Dna Fragmentation in Spermatozoa: Is There a Correlation?

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