Comparative analysis of the underground parts ofGentiana species by HPLC with diode-array and mass spectrometric detection

Szűcs, Zoltán; Dános, B.; Nyiredy, Sz.

doi:10.1007/bf02494108

Cited by 42 publications

(25 citation statements)

References 10 publications

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“…Szücs et al (2002) also reported that wild G. cruciata was characterized by high accumulation of gentiopicroside. Moreover, the quantities of swertiamarin and sweroside were low, while amarogentine, gentisin and gentioside isomers were not found in G. cruciata.…”

Section: Pcr Analysis Of Transgenic Hairy Rootsmentioning

confidence: 90%

“…In previous studies, the underground parts of all wild growing Gentiana species studied consisted of three secoiridoid-glycosides: gentiopicroside, swertiamarine and sweroside (Szücs et al 2002;Carnat et al 2005). Szücs et al (2002) also reported that wild G. cruciata was characterized by high accumulation of gentiopicroside.…”

Section: Pcr Analysis Of Transgenic Hairy Rootsmentioning

confidence: 92%

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Induction of Gentiana cruciata hairy roots and their secondary metabolites

et al. 2011

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Gentiana cruciata L. (cross gentian) is a medicinal and ornamental plant. The root extracts of this species are known to exhibit many curative properties. The natural Gentiana populations are exposed to great danger because of their uncontrolled usage. In this study, hairy roots from Gentiana cruciata L. stem and leaf explants belonging to three different clones were induced by inoculation with four different Agrobacterium rhizogenes wild strains namely A4, 15834, 8196 and R1000. Induction of the root transformation was significantly dependent on the explant type used. On the other hand, the genotype and bacterial strain had no significant effect on hairy root formation. Hairy root formation percentages of the explants varied between 5.6-33.3% in the stem explants, and between 0.0-6.7% in the leaf explants. Transformations of the hairy roots were confirmed by PCR using rolC specific primers, and revealed the absence of contaminating A. rhizogenes with virC primers. Total of twelve hairy root clones were obtained, and their secondary metabolite content was also analyzed by HPLC. Quantitative results exhibited that gentiopicroside was the most abundant compound in all root samples. Furthermore, metabolites such as loganic acid, swertiamarin, and sweroside were also identified and quantified in the samples.

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Section: Pcr Analysis Of Transgenic Hairy Rootsmentioning

confidence: 90%

Section: Pcr Analysis Of Transgenic Hairy Rootsmentioning

confidence: 92%

Induction of Gentiana cruciata hairy roots and their secondary metabolites

et al. 2011

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“…According to the studies in the literature, the underground parts of all the Gentiana species consist of the three secoiridoid-glycosides: gentiopicroside, swertiamarine, and sweroside (Szücs et al 2002;Carnat et al 2005). Moreover, Szücs et al (2002) reported that gentiopicroside was the main characteristic compound in G. cruciata, G. pneumonanthe, and G. asclepiadea, but the quantities of swertiamarine and sweroside were very small, while amarogentine, gentisine, and gentioside isomers were not found in G. cruciata.…”

Section: Resultsmentioning

confidence: 94%

“…Moreover, Szücs et al (2002) reported that gentiopicroside was the main characteristic compound in G. cruciata, G. pneumonanthe, and G. asclepiadea, but the quantities of swertiamarine and sweroside were very small, while amarogentine, gentisine, and gentioside isomers were not found in G. cruciata. Consequently, the results of the present study were in accordance with the published data, and it was concluded that the genotype might be an important factor for the quantity of the secondary metabolites present in the roots.…”

Section: Resultsmentioning

confidence: 95%

Shoot proliferation and HPLC-determination of iridoid glycosides in clones of Gentiana cruciata L

Hayta

Akgün

Ganzera

et al. 2011

Plant Cell Tiss Organ Cult

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Gentiana cruciata L. (Cross gentian) is a medicinal and ornamental plant, threatened in its natural habitats. The wild root extracts of this species are known to exhibit many curative properties. In the present study, an efficient protocol for in vitro propagation of G. cruciata L. was developed from node culture. A semi-solidified Murashige and Skoog (MS) basal medium supplemented with 2.22 lM 6-benzyladenine (BA), 2.46 lM indole-3-butyric acid (IBA) and sucrose (3% w/v) improved the production of multiple shoots directly from nodal segments, providing 3.9 shoots per explants on average. The highest rooting (81.7%) was observed with half-strength MS medium supplemented with 2.46 lM IBA. Plants with well-developed roots were transferred to pots containing turf/vermiculite mixtures and acclimatized in plant growth chamber conditions. Acclimatized plants showed 100% survival and remained healthy. The content of secondary metabolites in the clones was determined by HPLC, and the presence of gentiopicroside, loganic acid, swertiamarin, and sweroside in the samples was confirmed. Gentiopicroside was found to be the major compound.

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“…Since the grade of substitution of xanthones is characteristic for a genus, and the oxidation pattern is uniform within a particular section, [40] xanthones are very useful in plant systematics and chemotaxonomy. [46] Population Characteristics and Linear Morphometry [41] In addition, antimicrobial, anti-inflammatory, and antispasmodic activities are shown for isovitexin and isoorientin.…”

Section: Resultsmentioning

confidence: 99%

Chemical and Morphological Inter‐ and Intrapopulation Variability in Natural Populations of Gentiana pneumonanthe L.

Popović

Krstić-Milošević

Stefanović

et al. 2019

Chemistry & Biodiversity

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Inter-and intrapopulation variability in six natural populations of the rare species Gentiana pneumonanthe was examined based on morphological and chemical data. Population size and linear morphometric parameters differed significantly among populations, but without a clear connection to habitat conditions, i. e. water supply and light availability. Leaf shape varied from ovate to lanceolate in all populations, and one population was distinctive in having the largest number of leaves of transitional shape. HPLC analyses of six secondary metabolites were performed separately for belowground parts, and aboveground vegetative and reproductive parts of individual plants (6 populations × 7 individuals × 3 plant parts, n = 126) in order to examine differences at the population and individual levels. Three secoiridoids (swertiamarin (SWM), sweroside (SWZ), and gentiopicrin (GP)), one xanthone (mangiferin (MGF)), and two flavones (isoorientin (IO) and isovitexin (IV)) were detected and quantified in the analyzed samples: sweroside dominated in the aboveground reproductive part, mangiferin in the aboveground vegetative part, and gentiopicrin in the belowground part. At the population level, differences in contents of the analyzed chemicals among populations were significant only for a few metabolites. At the individual level, a pronounced organ-dependent distribution of secondary metabolites was revealed. The results of this study contribute to a better understanding of natural variability within populations of the rare and threatened G. pneumonanthe, and provide data on the contents and within-plant distribution of secondary metabolites, which are important as pharmacologically active compounds and may be useful for further biotechnological procedures regarding this species.

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Comparative analysis of the underground parts ofGentiana species by HPLC with diode-array and mass spectrometric detection

Cited by 42 publications

References 10 publications

Induction of Gentiana cruciata hairy roots and their secondary metabolites

Induction of Gentiana cruciata hairy roots and their secondary metabolites

Shoot proliferation and HPLC-determination of iridoid glycosides in clones of Gentiana cruciata L

Chemical and Morphological Inter‐ and Intrapopulation Variability in Natural Populations of Gentiana pneumonanthe L.

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