Comparative analysis of the proteome of left ventricular heart of arteriosclerosis in rat

Huang, Zhenyu; Yang, Pengyuan; Almofti, Mohamad Radwan; Yu, Yanling; Rui, Yao-Cheng

doi:10.1016/j.lfs.2004.04.054

Cited by 19 publications

(10 citation statements)

References 22 publications

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“…Qualification number is SCXK (hu) 2002-0010. The AS rat model was established by intraperitoneal injection with a single dose of vitamin D 3 600 kU/kg in addition to loading with high fat/cholesterol diet containing sodium cholate and propyl-thyracil as described previously [2] . The rats were divided into three groups: control, AS, and GbE.…”

Section: Methodsmentioning

confidence: 99%

“…The rat was considered suitable for establishing cardiovascular disease model including cardiac hypertrophy, hypertension, and heart failure; but it is not such a good model in atherosclerosis due to its hyperlipidemia-resistant property. Recently, our task-group established the experimental model of AS in rats by combining the high fat/cholesterol diets containing sodium cholate and propyl-thyracil with injection of vitamin D 3 [2] . Ginkgo biloba extract (GbE) has beneficial effects on cardiovascular disease.…”

Section: Introductionmentioning

confidence: 99%

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Expression of pro-inflammatory and anti-inflammatory cytokines in brain of atherosclerotic rats and effects of Ginkgo biloba extract1

Jiao

Rui

et al. 2005

Acta Pharmacologica Sinica

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Aim: To study the protein and mRNA expressions of pro-inflammatory and antiinflammatory cytokines in the brain of rats with atherosclerosis (AS) and the effects of Ginkgo biloba extract (GbE) on expressions of cytokines. Methods: The experimental model of AS in rats were established by intraperitioneal injection of vitamin D 3 with high fat/cholesterol diet. GbE 100 mg/kg was administered to rats by ig. After 8 weeks, the expressions of IL-1β, TNF-α, IL-10, and IL-10R in the brain tissues of AS rats were detected by enzyme-linked immunosorbant assay, immunohistochemistry, Western blotting, and reverse transcriptase polymerase chain reaction. Results: The protein and mRNA expressions of IL-1β, TNF-α, and IL-10 in the brains were markedly higher in AS groups than that in control groups (6.11±0.15, 1.55±0.14, 0.54±0.04 ng/g wet weight vs 0.80±0.14, 0.33±0.09, and 0.33±0.02 ng/g wet weight, respectively). The protein and mRNA expressions of IL-1β and TNF-α in the brains were markedly lower in GbE groups (3.82±0.54, 0.95±0.08 ng/g wet weight) than that in AS groups, the protein and mRNA expressions of IL-10 and IL-10R in the brains were markedly higher in GbE groups (0.85±0.06 ng/g wet weight) than that in AS groups. Conclusion: GbE inhibited production of pro-inflammatory cytokines IL-1β and TNF-α, but upregulated the production of anti-inflammatory cytokines, IL-10 and IL-10R in brain, which might be related with its anti-AS actions.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of pro-inflammatory and anti-inflammatory cytokines in brain of atherosclerotic rats and effects of Ginkgo biloba extract1

Jiao

Rui

et al. 2005

Acta Pharmacologica Sinica

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“…The rats in experimental groups described previously (Huang et al, 2004) were enterocoelia injected with a single dose of vitamin D 3 600,000 IU per kg body weight and loaded with high fat diet containing 3% cholesterol, 0.5% sodium cholate, 0.2% propyl-thyracil, 5% refined sugar, 10% lard, and 81.3% base feed. In 4 weeks, atherosclerotic plaques were seen in the aorta and in 6 weeks the atherosclerotic plaques in coronary arteries were formed.…”

Section: Model Of Atherosclerotic Ratsmentioning

confidence: 99%

“…The atherosclerotic rat model was established by injection with a single dose of vitamin D 3 in addition to loading with high fat diet (Huang et al, 2004). The high fat diet contained sodium cholate and protpyl-thracil besides cholesterol, sugar and lard.…”

Section: Pathologic Changes In Atherosclerotic Ratsmentioning

confidence: 99%

Time courses of vascular endothelial growth factor and intercellular adhesion molecule-1 expressions in aortas of atherosclerotic rats

et al. 2005

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“…Recently, proteomics has been widely used to investigate the protein patterns of different cardiovascular diseases, such as heart failure [1], dilated cardiomyopathy (DCM) [2], arteriosclerosis [3], and myocardial infarction of different animal models, such as dog [4], rabbit [5], and rat [6,7], and some identified proteins are demonstrated as having a correlation with the onset of diseases [8,9].…”

Section: Introductionmentioning

confidence: 99%

Different protein expression of myocardium from Chinese mini-swine model of myocardial infarct

Zhao

Zhang

et al. 2007

Front. Chem. China

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High-resolution two-dimensional gel electrophoresis (2-DE), followed by computer-assisted image analysis was used to screen protein patterns of normal and infarcted myocardial tissues for quantitative and qualitative differences in protein expression. In the gels of pH 5-8 immobilized pH gradient (IPG) strips, 851 protein spots were detected in normal myocardial tissue and 1 032 protein spots were resolved in infarcted myocardial tissue. Thirteen protein spots only expressed in normal myocardial tissue, and 14 protein spots only expressed in infarcted myocardial tissue. Results also showed that 49 protein spots displayed quantitative changes in expression between normal and infarcted myocardial tissue. Eleven protein spots were subjected to mass spectrometry (MS) analysis and seven proteins were identified by peptide mass fingerprinting (PMF). These proteins may be involved in cardiovascular injury, and could play an important role in the treatment of coronary heart disease.

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Comparative analysis of the proteome of left ventricular heart of arteriosclerosis in rat

Cited by 19 publications

References 22 publications

Expression of pro-inflammatory and anti-inflammatory cytokines in brain of atherosclerotic rats and effects of Ginkgo biloba extract1

Expression of pro-inflammatory and anti-inflammatory cytokines in brain of atherosclerotic rats and effects of Ginkgo biloba extract1

Time courses of vascular endothelial growth factor and intercellular adhesion molecule-1 expressions in aortas of atherosclerotic rats

Different protein expression of myocardium from Chinese mini-swine model of myocardial infarct

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