Comparative analysis of the plastid conversion, photochemical activity and chlorophyll degradation in developing embryos of green-seeded and yellow-seeded pea (Pisum sativum) cultivars

Smolikova, Galina; Shiroglazova, Olga; Vinogradova, G. Yu.; Leppyanen, I. V.; Dinastiya, Ekaterina M.; Yakovleva, O. V.; Долгих, Е. А.; Titova, G. E.; Frolov, Andrej; Медведев, С.С.

doi:10.1071/fp19270

Cited by 13 publications

(7 citation statements)

References 51 publications

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“…Interestingly, these genes are tightly co-expressed during all steps of plant ontogenesis [101]. ABI3 and ABI5 are involved in the regulation of chlorophyll catabolism, raffinose family oligosaccharides synthesis, and LEA genes expression at the late maturation stage [16,92,[102][103][104][105]. ABI5 can also act as a signaling integrator of ABA and other hormones [103,106,107].…”

Section: The Lafl Network and The Developmental Programmentioning

confidence: 99%

Seed-to-Seedling Transition in Pisum sativum L.: A Transcriptomic Approach

Smolikova

Стрыгина

Крылова

et al. 2022

Plants

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The seed-to-seedling transition is a crucial step in the plant life cycle. The transition occurs at the end of seed germination and corresponds to the initiation of embryonic root growth. To improve our understanding of how a seed transforms into a seedling, we germinated the Pisum sativum L. seeds for 72 h and divided them into samples before and after radicle protrusion. Before radicle protrusion, seeds survived after drying and formed normally developed seedlings upon rehydration. Radicle protrusion increased the moisture content level in seed axes, and the accumulation of ROS first generated in the embryonic root and plumule. The water and oxidative status shift correlated with the desiccation tolerance loss. Then, we compared RNA sequencing-based transcriptomics in the embryonic axes isolated from pea seeds before and after radicle protrusion. We identified 24,184 differentially expressed genes during the transition to the post-germination stage. Among them, 2101 genes showed more prominent expression. They were related to primary and secondary metabolism, photosynthesis, biosynthesis of cell wall components, redox status, and responses to biotic stress. On the other hand, 415 genes showed significantly decreased expression, including the groups related to water deprivation (eight genes) and response to the ABA stimulus (fifteen genes). We assume that the water deprivation group, especially three genes also belonging to ABA stimulus (LTI65, LTP4, and HVA22E), may be crucial for the desiccation tolerance loss during a metabolic switch from seed to seedling. The latter is also accompanied by the suppression of ABA-related transcription factors ABI3, ABI4, and ABI5. Among them, HVA22E, ABI4, and ABI5 were highly conservative in functional domains and showed homologous sequences in different drought-tolerant species. These findings elaborate on the critical biochemical pathways and genes regulating seed-to-seedling transition.

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Section: The Lafl Network and The Developmental Programmentioning

confidence: 99%

Seed-to-Seedling Transition in Pisum sativum L.: A Transcriptomic Approach

Smolikova

Стрыгина

Крылова

et al. 2022

Plants

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“…Chlorophyll fluorescence-based technology is centered on the capacity of chlorophyll, which is often present in seeds during their development to emit light in a slightly longer wavelength in relation to the light that was absorbed (Misra et al, 2012;Smolikova et al, 2017). Because chlorophyll degradation is usually observed at late steps of seed maturation (Smolikova et al, 2020), chlorophyll content and its fluorescence are inversely proportional to the seed maturity and, consequently, to its quality (Ooms and Destain, 2011).…”

Section: Introductionmentioning

confidence: 99%

Integrating Optical Imaging Tools for Rapid and Non-invasive Characterization of Seed Quality: Tomato (Solanum lycopersicum L.) and Carrot (Daucus carota L.) as Study Cases

et al. 2020

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Light-based methods are being further developed to meet the growing demands for food in the agricultural industry. Optical imaging is a rapid, non-destructive, and accurate technology that can produce consistent measurements of product quality compared to conventional techniques. In this research, a novel approach for seed quality prediction is presented. In the proposed approach two advanced optical imaging techniques based on chlorophyll fluorescence and chemometric-based multispectral imaging were employed. The chemometrics encompassed principal component analysis (PCA) and quadratic discrimination analysis (QDA). Among plants that are relevant as both crops and scientific models, tomato, and carrot were selected for the experiment. We compared the optical imaging techniques to the traditional analytical methods used for quality characterization of commercial seedlots. Results showed that chlorophyll fluorescence-based technology is feasible to discriminate cultivars and to identify seedlots with lower physiological potential. The exploratory analysis of multispectral imaging data using a non-supervised approach (two-component PCA) allowed the characterization of differences between carrot cultivars, but not for tomato cultivars. A Random Forest (RF) classifier based on Gini importance was applied to multispectral data and it revealed the most meaningful bandwidths from 19 wavelengths for seed quality characterization. In order to validate the RF model, we selected the five most important wavelengths to be applied in a QDA-based model, and the model reached high accuracy to classify lots with high-and low-vigor seeds, with a correct classification from 86 to 95% in tomato and from 88 to 97% in carrot for validation set. Further analysis showed that low quality seeds resulted in seedlings with altered photosynthetic capacity and chlorophyll content. In conclusion, both chlorophyll fluorescence and chemometrics-based multispectral imaging can be applied as reliable proxies of the physiological potential in tomato and carrot seeds. From the practical point of view, such techniques/methodologies can be potentially used for screening low quality seeds in food and agricultural industries.

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“…Of late, few common factors are ought to be involved in regulation of initiation and termination of dormancy along with enhancing seed maturation and desiccation processes, that include DOG1 proteins and ABA (Chahtane et al, 2017 ; Nonogaki, 2019 ; Smolikova et al, 2020 ). Hence, understanding of the activities and regulatory control of these key factors would facilitate better understanding of seed longevity.…”

Section: Candidate Genes/proteins Affecting Seed Longevity In Legumesmentioning

confidence: 99%

Seed Longevity in Legumes: Deeper Insights Into Mechanisms and Molecular Perspectives

et al. 2022

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Sustainable agricultural production largely depends upon the viability and longevity of high-quality seeds during storage. Legumes are considered as rich source of dietary protein that helps to ensure nutritional security, but associated with poor seed longevity that hinders their performance and productivity in farmer's fields. Seed longevity is the key determinant to assure proper seed plant value and crop yield. Thus, maintenance of seed longevity during storage is of prime concern and a pre-requisite for enhancing crop productivity of legumes. Seed longevity is significantly correlated with other seed quality parameters such as germination, vigor, viability and seed coat permeability that affect crop growth and development, consequently distressing crop yield. Therefore, information on genetic basis and regulatory networks associated with seed longevity, as well as molecular dissection of traits linked to longevity could help in developing crop varieties with good storability. Keeping this in view, the present review focuses towards highlighting the molecular basis of seed longevity, with special emphasis on candidate genes and proteins associated with seed longevity and their interplay with other quality parameters. Further, an attempt was made to provide information on 3D structures of various genetic loci (genes/proteins) associated to seed longevity that could facilitate in understanding the interactions taking place within the seed at molecular level. This review compiles and provides information on genetic and genomic approaches for the identification of molecular pathways and key players involved in the maintenance of seed longevity in legumes, in a holistic manner. Finally, a hypothetical fast-forward breeding pipeline has been provided, that could assist the breeders to successfully develop varieties with improved seed longevity in legumes.

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Comparative analysis of the plastid conversion, photochemical activity and chlorophyll degradation in developing embryos of green-seeded and yellow-seeded pea (Pisum sativum) cultivars

Cited by 13 publications

References 51 publications

Seed-to-Seedling Transition in Pisum sativum L.: A Transcriptomic Approach

Seed-to-Seedling Transition in Pisum sativum L.: A Transcriptomic Approach

Integrating Optical Imaging Tools for Rapid and Non-invasive Characterization of Seed Quality: Tomato (Solanum lycopersicum L.) and Carrot (Daucus carota L.) as Study Cases

Seed Longevity in Legumes: Deeper Insights Into Mechanisms and Molecular Perspectives

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