Comparative Analysis of the Organization of the Cholinergic System in the Brains of Two Holostean Fishes, the Florida Gar &lt;b&gt;&lt;i&gt;Lepisosteus platyrhincus&lt;/i&gt;&lt;/b&gt; and the Bowfin &lt;b&gt;&lt;i&gt;Amia calva&lt;/i&gt;&lt;/b&gt;

Morona, Ruth; López, Jesús M.; Northcutt, R. Glenn; González, Agustı́n

doi:10.1159/000347111

Cited by 14 publications

(40 citation statements)

References 188 publications

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“…The specificity of the ChAT, TH, CB, and CR antisera has been previously described in L. platyrhincus [Morona et al, 2013], and a similar pattern of labeling for these markers has been detected in L. oculatus (present results).…”

Section: Double Brightfield Labeling For Nadph-d and Chat Or Thsupporting

confidence: 89%

“…The nomenclature used is essentially the same as in previous studies of the brain in the genus Lepisosteus [Northcutt, 1982;Parent and Northcutt, 1982;Song and Northcutt, 1991;Northcutt and Butler, 1993;Collin and Northcutt, 1995;Meek and Nieuwenhuys, 1998;Chiba and Oka, 1999;Baker and Bird, 2002;Chiba, 2005;Morona et al, 2013], including the terminology of the prosomeric model for the forebrain [Puelles andRubenstein, 2003, 2015].…”

Section: Evaluation and Presentation Of The Resultsmentioning

confidence: 99%

“…In particular, there are limited hodological data related to the visual and the lateral line systems Butler, 1976, 1980;Song and Northcutt, 1991;Collin and Northcutt, 1995], and several mapping studies of the distribution of monoamines [Parent and Northcutt, 1982;Chiba and Oka, 1999] and diverse neuropeptides [Malz et al, 1999;Baker and Bird, 2002;Chiba, 2005]. In addition, in a previous study we described in detail the organization of the brain cholinergic system [Morona et al, 2013].…”

Section: Introductionmentioning

confidence: 99%

“…This neuroanatomical information is a useful tool for distinguishing and defining classes of neurons and cell groups within the poorly investigated brain of gars. In addition, double-labeling experiments for NOS or NADPH-d and choline acetyltransferase (ChAT; the synthesis enzyme of acetylcholine), tyrosine hydroxylase (TH; the first and rate-limiting enzyme for catecholamine synthesis), serotonin (5-HT), and the calcium binding proteins calbindin (CB) and calretinin (CR), were conducted to locate more precisely the nitrergic cell populations and to evaluate some neurochemical features of the NOS immunoreactive (NOS-ir) neurons, given the previous descriptions of the localization of these neuroactive molecules in the brain of gars [Chiba and Oka, 1999;Morona et al, 2013;González et al, 2014]. The results are compared with those reported in other groups of fishes and tetrapods to establish conserved and specific features along their evolution, and therefore to increase the current knowledge of this neurotransmitter system.…”

Section: Introductionmentioning

confidence: 99%

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Pattern of Nitrergic Neuronal System Organization in the Brain of Two Holostean Fishes (Actinopterygii: Ginglymodi)

et al. 2017

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The study of the nitrergic system, formed by the networks of neurons containing the enzyme nitric oxide synthase (NOS), has been extremely useful in unraveling neuroanatomical features of the organization of the central nervous system of vertebrates. Thus, data are available for representatives of most vertebrate classes and, in particular, several studies have detailed the organization of this system in teleosts. In contrast, no information is available regarding this neurotransmission system in the brains of holosteans, an early diverged and poorly understood group of actinopterygian fishes, currently considered a sister group of teleosts that contains only 8 species. The present study provides the first detailed information on the distribution of nitrergic cell bodies and fibers in 2 holostean species of the genus Lepisosteus, the spotted gar L. oculatus and the Florida gar L. platyrhincus. NOS immunohistochemistry and the NADPH diaphorase (NADPH-d) histochemical reaction were used, and both techniques yielded identical results, with the exception of the primary olfactory and terminal nerve fibers, which only labeled for NADPH-d exclusively in L. oculatus. Double immunohistochemistry was conducted for the simultaneous detection of NOS with tyrosine hydroxylase, choline acetyltransferase, calbindin, calretinin, and serotonin to accurately establish the localization of the nitrergic neurons and fibers in the brain of holosteans, the neuroanatomy of which has been mostly neglected, and to assess possible interactions between these neuroactive substances. Distinct groups of nitrergic cells were located in subpallial areas, the basal hypothalamus, posterior tubercle, optic tectum and mesencephalic tegmentum, reticular formation, solitary tract nucleus, spinal cord, and amacrine cells in the retina. In addition, low numbers of nitrergic cells were observed in the pallium, suprachiasmatic nucleus, prethalamic and thalamic areas, torus lateralis and torus semicircularis, cerebellar and laterodorsal tegmental nuclei, and the ventral octavolateral area. Comparison of these results with those from other classes of vertebrates, and including a segmental analysis to correlate cell populations, reveals that the pattern of the nitrergic system in holosteans is very close to that in ancestral actinopterygian fishes and highlights conserved and derived traits.

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Section: Double Brightfield Labeling For Nadph-d and Chat Or Thsupporting

confidence: 89%

Section: Evaluation and Presentation Of The Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pattern of Nitrergic Neuronal System Organization in the Brain of Two Holostean Fishes (Actinopterygii: Ginglymodi)

et al. 2017

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“…The most recent analysis of vertebrate phylogeny inferred from multiple nuclear DNA-coded genes has shown that living holosteans, comprising bowfins and gars, form a monophyletic group which is the sister group to teleost fishes [see citations in Morona et al, 2013]. Although the pallium of Amia is everted to a much greater extent than in gars, the subpallium is organized in a similar manner, which is closely related to that described in Polypterus .…”

Section: Actinopterygiansmentioning

confidence: 99%

Identification of Striatal and Pallidal Regions in the Subpallium of Anamniotes

et al. 2014

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The telencephalic basal ganglia (BG) of amniotes consist of two subdivisions, striatum and pallidum, which share many features, including development, cell types, neurotransmitter organization and hodology. In particular, these two subdivisions during development are defined on the basis of discrete gene expression patterns (genoarchitecture or genoarchitectonics). The characterization of the BG in the subpallium of representatives of the different classes of anamniote vertebrates was first approached in studies dealing with their localization, hodology and main neurochemical characteristics. Thus, it was proposed that an impressive degree of conservation exists across species. New insights can be gained by the comparative analysis of the expression of conserved transcription factors that distinctly define the striatal and pallidal components of the BG in all vertebrates. In addition, the expression of other genes that characterize neighboring regions of the BG is also useful to define the boundaries of each subdivision. Following this approach, we have analyzed the BG in the brain of juvenile representatives of amphibians, lungfishes, holosteans, Polypteriformes and Chondrichthyes. In addition, we briefly review previous data in teleosts and agnathans. The markers used include islet 1 and Dlx as striatal markers, whereas Nkx2.1 is essential for the identification of the pallidum. In turn, Pax6 and in particular Tbr1 are expressed in the pallium. These markers have been systematically analyzed in combination with neuronal markers of specific subpallial territories and cell populations, such as tyrosine hydroxylase, γ-aminobutyric acid, nitric oxide synthase, substance P and enkephalin. The results highlight that many genes share common distribution patterns and are arranged in conserved combinations whose identification has served to define homologies between components of the BG in distant species.

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A hindbrain segmental scaffold specifying neuronal location in the adult goldfish, Carassius auratus

Gilland

Wong

Baker

et al. 2014

J of Comparative Neurology

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The vertebrate hindbrain develops as a series of well-defined neuroepithelial segments or rhombomeres. While rhombomeres are visible in all vertebrate embryos, generally there is not any visible segmental anatomy in the brains of adults. Teleost fish are exceptional in retaining a rhombomeric pattern of reticulospinal neurons through embryonic, larval, and adult periods. We use this feature to map more precisely the segmental imprint in the reticular and motor basal hindbrain of adult goldfish. Analysis of serial sections cut in three planes and computer reconstructions of retrogradely labeled reticulospinal neurons yielded a segmental framework compatible with previous reports and more amenable to correlation with surrounding neuronal features. Cranial nerve motoneurons and octavolateral efferent neurons were aligned to the reticulospinal scaffold by mapping neurons immunopositive for choline acetyltransferase or retrogradely labeled from cranial nerve roots. The mapping corresponded well with the known ontogeny of these neurons and helps confirm the segmental territories defined by reticulospinal anatomy. Because both the reticulospinal and the motoneuronal segmental patterns persist in the hindbrain of adult goldfish, we hypothesize that a permanent "hindbrain framework" may be a general property that is retained in adult vertebrates. The establishment of a relationship between individual segments and neuronal phenotypes provides a convenient method for future studies that combine form, physiology, and function in adult vertebrates.

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Comparative Analysis of the Organization of the Cholinergic System in the Brains of Two Holostean Fishes, the Florida Gar <b><i>Lepisosteus platyrhincus</i></b> and the Bowfin <b><i>Amia calva</i></b>

Cited by 14 publications

References 188 publications

Pattern of Nitrergic Neuronal System Organization in the Brain of Two Holostean Fishes (Actinopterygii: Ginglymodi)

Pattern of Nitrergic Neuronal System Organization in the Brain of Two Holostean Fishes (Actinopterygii: Ginglymodi)

Identification of Striatal and Pallidal Regions in the Subpallium of Anamniotes

A hindbrain segmental scaffold specifying neuronal location in the adult goldfish, Carassius auratus

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