Comparative analysis of the miRNA profiles from Taenia solium and Taenia asiatica adult

Ai, Lin; Chen, Mu-Xin; Zhang, Yongnian; Chen, Shaohong; Zhou, Xiao-Nong; Chen, Jiaxu

doi:10.5897/ajmr12.1218

Cited by 6 publications

(2 citation statements)

References 27 publications

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“…It was in concordance with the results of other cestodes, such as Mesocestoides corti, Echinococcus multilocularis , and Echinococcus canadensis [41,42]. In contrast, in Taenia adult stage, miR-7-5p, miR-71, miR-277, miR-219-5p, and miR-2b-3p were predominant in T. solium and T. asiatica , and miR-71 and miR-219-5p were the most abundant in T. saginata [25,26]. Notably, miR-71 was localized in the same cluster with miR-2b and miR-2c, while miR-4849 was in the same cluster with miR-277; these clusters are conserved across Cestodes [24,43].…”

Section: Discussionmentioning

confidence: 99%

Taenia soliumandTaenia crassiceps: miRNomes of the larvae and effects of miR-10-5p and let-7-5p on murine peritoneal macrophages

et al. 2019

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Neurocysticercosis (NCC), a major cause of neurological morbidity worldwide, is caused by the larvae of Taenia solium. Cestodes secrete molecules that block the Th1 response of their hosts and induce a Th2 response permissive to their establishment. Mature microRNAs (miRs) are small noncoding RNAs that regulate gene expression and participate in immunological processes. To determine the participation of Taenia miRs in the immune response against cysticercosis, we constructed small RNA (sRNA) libraries from larvae of Taenia solium and Taenia crassiceps. A total of 12074504 and 11779456 sequencing reads for T. solium and T. crassiceps, respectively, were mapped to the genomes of T. solium and other helminths. Both larvae shared similar miRNome, and miR-10-5p was the most abundant in both species, followed by let-7-5p in T. solium and miR-4989-3p in T. crassiceps, whereas among the genus-specific miRs, miR-001-3p was the most abundant in both, followed by miR-002-3p in T. solium and miR-003a-3p in T. crassiceps. The sequences of these miRs were identical in both. Structure and target prediction analyses revealed that these pre-miRs formed a hairpin and had more than one target involved in immunoregulation. Culture of macrophages, RT-PCR and ELISA assays showed that cells internalized miR-10-5p and let-7-5p into the cytoplasm and the miRs strongly decreased interleukin 16 (Il6) expression, tumor necrosis factor (TNF) and IL-12 secretion, and moderately decreased nitric oxide synthase inducible (Nos2) and Il1b expression (pro-inflammatory cytokines) in M(IFN-γ) macrophages and expression of Tgf1b, and the secretion of IL-10 (anti-inflammatory cytokines) in M(IL-4) macrophages. These findings could help us understand the role of miRs in the host–Taenia relationship.

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Section: Discussionmentioning

confidence: 99%

Taenia soliumandTaenia crassiceps: miRNomes of the larvae and effects of miR-10-5p and let-7-5p on murine peritoneal macrophages

et al. 2019

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“…A comparative analysis of the miRNAs profile between T. saginata and T. solium adult worms revealed a high similarity between these two species, most surely due to their close identity, with predominant expression of miR-7-5p, miR-71, miR-277, miR-219-5p, and miR-2b-3p. Of note, miR-10-5p was a read unique for T. solium (Lin et al, 2014 ). We can elaborate that this reflects differences in physiology or life cycle.…”

Section: Mirnas In Taenia Solium : Molecular Signamentioning

confidence: 99%

MicroRNAs in Taenia solium Neurocysticercosis: Insights as Promising Agents in Host-Parasite Interaction and Their Potential as Biomarkers

et al. 2017

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MicroRNAs (miRNAs) are short, endogenous, non-coding, single-stranded RNAs involved in post-transcriptional gene regulation. Although, several miRNAs have been identified in parasitic helminths, there is little information about their identification and function in Taenia. Furthermore, the impact of miRNAs in neurocysticercosis, the brain infection caused by larvae of Taenia solium is still unknown. During chronic infection, T. solium may activate numerous mechanisms aimed to modulate host immune responses. Helminthic miRNAs might also have effects on host mRNA expression and thus play an important role regulating host-parasite interactions. Also, the diagnosis of this disease is difficult and it usually requires neuroimaging and confirmatory serology. Since miRNAs are stable when released, they can be detected in body fluids and therefore have potential to diagnose infection, determine parasite burden, and ascertain effectiveness of treatment or disease progression, for instance. This review discusses the potential roles of miRNAs in T. solium infection, including regulation of host-parasite relationships and their eventual use as diagnostic or disease biomarkers. Additionally, we summarize the bioinformatics resources available for identification of T. solium miRNAs and prediction of their targets.

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Cestodes in the genomic era

2021

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Comparative analysis of the miRNA profiles from Taenia solium and Taenia asiatica adult

Cited by 6 publications

References 27 publications

Taenia soliumandTaenia crassiceps: miRNomes of the larvae and effects of miR-10-5p and let-7-5p on murine peritoneal macrophages

Taenia soliumandTaenia crassiceps: miRNomes of the larvae and effects of miR-10-5p and let-7-5p on murine peritoneal macrophages

MicroRNAs in Taenia solium Neurocysticercosis: Insights as Promising Agents in Host-Parasite Interaction and Their Potential as Biomarkers

Cestodes in the genomic era

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