Comparative analysis of the membrane-active properties of gossypol derivatives and their polymeric complexes

Гордиенко, Наталья Викторовна; Zamaraeva, Maria; Gagel’gans, A. I.; Baram, N. I.; Исмаилов, А. И.; Biktimirov, A.; Ziyaev, Kh. L.

doi:10.1007/bf01165192

Cited by 1 publication

(6 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We demonstrated that 1 caused change in structural order in the hydrophobic part of the erythrocyte membrane. Previously we observed this effect of 1 in liposomes . Therefore, we hypothesize that 1 induced erythrocyte hemolysis via interaction with membrane lipids.…”

Section: Discussionsupporting

confidence: 52%

“…Previously we observed this effect of 1 in liposomes. 41 Therefore, we hypothesize that 1 induced erythrocyte hemolysis via interaction with membrane lipids.…”

Section: Discussionmentioning

confidence: 97%

“…Experiments with the fluorescent probe AHC located on the surface of the lipid bilayer showed that 2 displaced the probe from the membrane to a lesser extent than 1 . This indirectly indicates a decreased affinity of 2 for lipids . Apparently, the hydrophobic parts of the 1 molecule in the complex with PVP ( 2 ) are screened by the polymer molecules, decreasing the affinity of 1 for lipids and consequently reducing the rate at which it accumulates in the membranes.…”

Section: Discussionmentioning

confidence: 99%

“…This indirectly indicates a decreased affinity of 2 for lipids. 41 Apparently, the hydrophobic parts of the 1 molecule in the complex with PVP (2) are screened by the polymer molecules, decreasing the affinity of 1 for lipids and consequently reducing the rate at which it accumulates in the membranes.…”

Section: Discussionmentioning

confidence: 99%

“…The filters were rinsed twice with water to remove the free radiolabeled compounds and dried at room temperature for 24 h. The filters were transferred to appropriate vials with 5 mL scintillation medium having the following composition: PPO, 5.0 g; POPOP, 0.125 g; toluene, 66.7 mL; Triton X-100, 333.3 mL and used to determine the radioactivity in a scintillation counter (Beta-1, Russia). Protein content was determined using Biuret reagent …”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

The Immobilization of Gossypol Derivative on N-Polyvinylpyrrolidone Increases its Water Solubility and Modifies Membrane-Active Properties

et al. 2009

View full text Add to dashboard Cite

The conjugate of the gossypol derivative megosin (1) with N-polyvinylpyrrolidone named rometin (2) was synthesized. The effects of 1 and 2 on the structure and permeability of human erythrocytes and rat liver mitochondria were compared. Compound 1 induced dose-dependent erythrocyte hemolysis and increased mitochondrial permeability, with concomitant changes in membrane structure as determined by ESR and fluorescence anisotropy methods. Immobilization of 1 on N-polyvinylpyrrolidone (compound 2) increased its water solubility and reduced the intensity of its effects on erythrocyte membrane integrity and mitochondrial permeability, which correlated with a decrease in the membranes structural changes induced by the compound. Although the same concentrations of free and N-polyvinylpyrrolidone bound 1 were used, far less (14)C-labeled 1 was incorporated into the membranes from complex than free 1. The increase in water solubility and the reduction of membrane-active properties of 1 after immobilization on N-polyvinylpyrrolidone could explain our previous observation of the decreased toxicity of 1.

show abstract

Section: Discussionsupporting

confidence: 52%

“…Previously we observed this effect of 1 in liposomes. 41 Therefore, we hypothesize that 1 induced erythrocyte hemolysis via interaction with membrane lipids.…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations