Comparative analysis of the main active components and hypoglycemic effects after the compatibility of Scutellariae Radix and Coptidis Rhizoma

Abstract

In this study, a rapid and highly sensitive ultra high performance liquid chromatography with triple quadrupole mass spectrometry method with the mobile phase of acetonitrile and 0.1% aqueous formic acid was established and successfully applied to comparatively analyze main active components after their compatibility. Besides, the effects of Scutellariae Radix, Coptidis Rhizoma and combined extracts on type 2 diabetic rats induced by high-fat diet along with low dose of streptozocin were investigated. Under the optimized chromatographic conditions, good separation of seven target components was achieved within 12 min. All calibration curves exhibited good linearity (R 2 ≥ 0.999). The relative standard deviation of precision, repeatability and stability varied from 0.69 to 2.23, 0.98 to 2.56, and 0.92 to 2.57%, respectively. The recovery ranged from 91.11 to 105.35%. The contents of seven active components were notably reduced after compatibility; however, the hypoglycemic effect of combined extracts was stronger than single drug by decreasing the activities of fructose-1,6-bisphosphatase, glucose 6-phosphatase, phosphoenolpyruvate carboxykinase and increasing the activities of glucokinase, phosphofructokinase, pyruvate kinase. Accordingly, the established analytical method was accurate and sensitive enough for quantitative evaluation of seven investigated compounds. Moreover, the combined extract had definite effects on type 2 diabetes through multiple components against multiple targets.

Traditional Chinese medicine for transformation of gastric precancerous lesions to gastric cancer: A critical review

“…These TCMs are usually combined to form a TCM compound for the clinical treatment of precancerous lesions, such as WPX, Sancao Tiaowei decoction, and Guiqi Baizhu prescriptions. Interestingly, the mechanisms of action of these active ingredients and TCM prescriptions in the treatment of precancerous lesions are not the same, which suggests that the curative effects of TCM are not caused by single chemical entities but result from their multi-ingredient prescription[ 123 ]. Chinese medicine differs from Western medicine in that many compounds in Chinese medicine act on multiple targets simultaneously, producing significant therapeutic effects[ 124 ].…”

Hypoglycemic effect of Taraxacum officinale root extract and its synergism with Radix Astragali extract

“…Currently, researchers have begun to concern about drug combination treatment in diabetes (Johnson et al., 2019; Li & Zhang, 2016; Schlosser, 2019). Studies have shown that the components and contents of the biologically active compounds will change after combination, and the interactions between these compounds may make better therapeutic effects, with fewer side effects due to the decrease in dose of each drug (Cui et al., 2019). As reported previously, RAE has shown a certain hypoglycemic effect and been commonly applied to treat diabetes as traditional Chinese medicine.…”

A systematic analysis of natural α-glucosidase inhibitors from flavonoids of Radix scutellariae using ultrafiltration UPLC-TripleTOF-MS/MS and network pharmacology

“…Many previous studies have investigated the antidiabetic constituents of Radix scutellariae. For instance, Cui et al used a tandem quadrupole mass spectrometer coupled with enzyme activity analysis to explore hypoglycemic effect of RS and Coptidis rhizome [90]. This method was accurate and sensitive enough for quantitative evaluation of seven major components and six enzymes.…”