Comparative analysis of the functional activity and composition of hydrolytic microbial complexes from the lower Volga barrow and modern chestnut soils

Manucharova, N. A.; Koltsova, E.M.; Степанов, А. Л.; Demkina, E. V.; Демкин, В. А.; Эль-Регистан, Г. И.

doi:10.1134/s002626171405018x

Cited by 9 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The addition of the chitin biopolymer to the paleovolcanic soil caused the indicators of the metabolically active prokaryotes share to reach 50% of the detected prokaryote biomass. Similar patterns were noted by the authors earlier for the microbial hydrolytic community of buried paleochannels compared to modern chestnut soils [20]. When polysaccharide was applied to chestnut paleosoils, the proportion of metabolically active prokaryotic cells reached 50% of the total detectable prokaryotic community, and the response to substrate application was more intense compared to modern soils.…”

Section: Discussionsupporting

confidence: 87%

“…The number and biomass of Actinobacteria doubled in the bacterial hydrolytic complex of stratified ochre paleosoils compared to its modern counterpart. The development of actinobacteria on such an inaccessible polysaccharide, nitrogen-containing biopolymer as chitin has been repeatedly noted by the authors [7,20]. The functional role of mycelial actinobacteria in the metabolism of chitin consists, on the one hand, of the active decomposition of this biopolymer, and, on the other hand, of the regulation of microbial hydrolytic complex activity through the production of biologically active regulatory metabolites, which occurs in a wide range of environmental parameters (moisture, temperature, organic matter, successional time).…”

Section: Discussionmentioning

confidence: 85%

“…Thus, earlier, the authors found that, in the microbial complex of buried chestnut soils, in comparison with their modern counterparts, the number of spore-forming actinobacteria increases noticeably. In addition, with the introduction of chitin, this pattern was significantly enhanced [20].…”

Section: Resultsmentioning

confidence: 96%

“…Epifluorescence microscopy using different fluorochromes-acridine orange, Sy3-was used to determine the total number and the number of metabolically active cells in the studied samples [20]. The total number of prokaryotes was determined using acridine orange dye, which reacts with cell DNA.…”

Section: Methodsmentioning

confidence: 99%

“…where N is the number of prokaryotic cells in 1 g of soil sample of bacteria in 1 g of sample, and the biomass of dry matter for 1 bacterial cell with volume of 0.1 µm 3 is 2 × 10-14 g [20].…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

The Prokaryotic Complex of Modern and Buried Soils on the Kamchatka Peninsula

Manucharova

Karimov

Певзнер

et al. 2022

Forests

View full text Add to dashboard Cite

A prokaryotic heterotrophic mesophilic community was studied in volcanic soil samples from Kamchatka. A phylogenetic and physiological characterization of the prokaryotic complex of modern and buried soils of the Kamchatka Peninsula is given. Volcanic Paleolithic soils (2500 and 11,300 years old) and their modern equivalents were investigated. It was found that the biomass of metabolically active prokaryotes in modern volcanic and Paleolithic soils reached 50 and 40 µg/g, respectively. The proportion of archaea in the metabolically active prokaryotic complex varied from 20% to 30% and increased in variants with the application of the nitrogen-containing biopolymer chitin. The application of the additional resource to paleovolcanic soils led to an incremental increase in the proportion of metabolically active prokaryotes, which reached 50% of the total prokaryotic biomass detected, indicating the high metabolic potential of the considered soils. Phylogenetic structure characteristics of the prokaryotic metabolically active component of modern and buried volcanic soil were established by molecular biology methods (metagenomic analysis, FISH method). The phylum Proteobacteria (74%), Acidobacteria, and Actinobacteria (14% combined) were dominant in modern soils; phylum Acidobacteria (51.8%) was dominant in paleosoils, whereas Chloroflexi (21%) and Proteobacteria (9%) were subdominant. It was determined that the potential activity of the microbial hydrolytic community, as measured by the relative response to the added resource (chitin), was found to increase in a series from modern to paleovolcanic soil. It was demonstrated that several key genes of the nitrogen cycle responsible for the processes of molecular nitrogen fixation, nitrification, and denitrification (nifH, amoA, nirK) were present in both modern and buried horizons.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations