Comparative analysis of the frequency of house dust mite specific and nonspecific Th1 and Th2 cells in skin lesions and peripheral blood of patients with atopic dermatitis

Neumann, Ch.; Gutgesell, C.; Fliegert, Frank; Bonifer, R; Herrmann, Franziska

doi:10.1007/s001090050041

Cited by 18 publications

(26 citation statements)

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“…After two washes in cold PBS, the samples were fixed in 2% formaldehyde. Specific controls used were PBMCs from all patient groups stained with an HLA-A*0201-tetramer based on an irrelevant peptide (hepatitis B core [18][19][20][21][22][23][24][25][26][27] and PBMCs from HLA-A*0201-negative individuals from all patient groups stained with the A*0201-Der p 1 tetramers. In line with previous reports (43), the mean plus 3 SDs of tetramer-binding cells in the PBMCs from all control individuals was less than 0.02% of CD8 + T cells.…”

Section: Methodsmentioning

confidence: 99%

“…Dust mite-specific CD4 + T cell responses have been identified and the HLA class II-restricting elements defined for several Der P antigens (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25). The responses have been found in blood and lesional skin of affected individuals and following dust mite patch test challenge (21,26). A significant proportion of the allergen-specific T cell clones isolated from lesional skin are of a CD8 + phenotype (26).…”

Section: Introductionmentioning

confidence: 99%

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Allergen-specific CD8+ T cells and atopic disease

Seneviratne¹,

Jones²,

King³

et al. 2002

J. Clin. Invest.

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Considerable evidence suggests that IL-10 may have a role in the manifestation of atopic disease. We sought to test the hypothesis that at the single cell level, allergen-specific T cells have diminished IL-10 production capacity in severely affected atopics compared with asymptomatic atopics. We defined three A*0201-restricted Der p 1 CD8+ T cell epitopes. Using human leukocyte antigen-A*0201–peptide (HLA-A*0201–peptide) tetrameric complexes and enzyme-linked immunospot assays to analyze peripheral blood mononuclear cells from A*0201-positive severely symptomatic atopics, asymptomatic atopics, and nonatopic controls, we observed a significant association between the frequency of the Der p 1–specific CD8+ T cells and disease activity. The specific T cells expressed an antigen-experienced cell surface phenotype, and 45.7% were positive for cutaneous lymphocyte-associated antigen. The specific T cells were able to produce IFN-γ efficiently, but their IL-10 production was significantly reduced in severely affected atopics. In contrast, viral-specific CD8+ T cells were able to produce equivalent amounts of IL-10 in the severely affected atopics compared with asymptomatic atopics and nonatopics. Through defining the first human atopic allergen HLA class I epitopes, we have provided a possible cellular mechanism to link the previous association of low IL-10 levels and severe atopic disease. These data are consistent with a role for CD8+ T cells in atopic disease pathogenesis and may provide a basis for future T cell immunotherapy strategies

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Allergen-specific CD8+ T cells and atopic disease

Seneviratne¹,

Jones²,

King³

et al. 2002

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…The use of peanut-specific clones or cell lines in these studies was justified by the fact that circulating allergen-specific T cells are present at a very low frequency among PBMCs (8,9). Nevertheless, cloning (or establishing cells lines) typically involves several cycles of Th stimulation in the presence of antigen, feeder cells, and…”

Section: Introductionmentioning

confidence: 99%

Characterization of lymphocyte responses to peanuts in normal children, peanut-allergic children, and allergic children who acquired tolerance to peanuts

Turcanu¹,

Maleki²,

Lack³

2003

J. Clin. Invest.

124

121

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“…5 Studies have been performed with PBMCs in which allergen-specific proliferation and, to a minor extent, cytokine production could be measured. 6,7 Cloning of antigen-specific T cells has been proved to be a powerful tool to investigate the immune response to allergens at the T-cell level.…”

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confidence: 99%

Cow's milk?specific T-cell reactivity of children with and without persistent cow's milk allergy: Key role for IL-10

Tiemessen

2004

Journal of Allergy and Clinical Immunology

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Background: The role of antigen-specific T cells in the mechanism of food allergy or maintenance of tolerance toward an innocuous antigen, such as cow's milk, is not yet fully understood. Objective: The cow's milk-specific T-cell response of donors with various allergic backgrounds was investigated. Methods: Cow's milk-specific T-cell clones (TCCs) were generated from the blood of children with persistent cow's milk allergy (CMA) and the blood of cow's milk-tolerant allergic and nonallergic control subjects. The TCCs were characterized by their antigen-specific proliferation, cytokine production, and activation status. Results: Cow's milk-specific TCCs of children with persistent CMA were T H 2 skewed, and the production of IL-4 and IL-13 was significantly correlated with the expression of the activation marker CD25. TCCs of the allergic control subjects were characterized by a high production of IL-10, which was positively correlated with the production of IL-4 and IFN-g and with the expression of CD25. TCCs derived from nonallergic control subjects had an attenuated response toward cow's milk in that they did not produce high levels of cytokines nor did they express high levels of surface markers. As in the allergic control subjects, in the nonallergic control subjects IL-10 production was positively correlated with the expression of CD25. Conclusion: The activation status of T cells derived from persistent donors with CMA was associated with the production of IL-4 and IL-13, whereas activated TCCs of cow's milktolerant control subjects were characterized by the production of IL-10 and, to a lesser extent, IFN-g. These findings suggest that activated CD4 + T cells (characterized by a high CD25 expression) might contribute to the tolerogenic immune response toward an antigen, such as cow's milk, through the production of

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Comparative analysis of the frequency of house dust mite specific and nonspecific Th1 and Th2 cells in skin lesions and peripheral blood of patients with atopic dermatitis

Cited by 18 publications

References 0 publications

Allergen-specific CD8+ T cells and atopic disease

Allergen-specific CD8+ T cells and atopic disease

Characterization of lymphocyte responses to peanuts in normal children, peanut-allergic children, and allergic children who acquired tolerance to peanuts

Cow's milk?specific T-cell reactivity of children with and without persistent cow's milk allergy: Key role for IL-10

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