Comparative analysis of the catalysis and stability of the native, recombinant and metagenomic alkaline proteases in organic solvents

Purohit, Megha K.; Rathore, Dalip Singh; Koladiya, Gopi A.; Pandey, Sandeep; Singh, Satya Prakash

doi:10.1007/s11356-022-21411-7

Cited by 2 publications

(1 citation statement)

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“…The study therefore suggests that the potential microbial sources from the extreme niches could provide hotbed for robust enzymes. [32][33][34][35][36]…”

Section: Application Of the Amylasementioning

confidence: 99%

Purification and Characterization of an Amylase from a Newly Isolated Geobacillus thermoleovorans TTIO4 from the Hot Spring of Tuwa‐Timba

Shukla

Singh

2023

Starch Stärke

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A thermophilic amylase produced by a newly isolated Geobacillus thermoleovorans TTIO4 from a hot spring in India is investigated. The isolate TTIO4 optimally produces extracellular amylase at 55 °C, pH 7, and 24 h of growth. The amylase is purified by 42 fold with a yield of 9.7%. The enzyme has a molecular weight of 40 kDa. The purified amylase catalyzes the reaction optimally at 60 °C and pH 7. Besides, it displays maximum thermal stability at 50 °C for 18 h. While the catalyst is improved by Ca+2, it is wholly inhibited by Hg+2. Furthermore, the chelators, inhibitors and solvents adversely affect the enzyme activity. The amylase is stabilized by SDS, while other surfactants negatively affect the enzyme. Km, Vmax, Kcat, and Kcat/Km of the amylase are 0.26 mg mL−1, 192.3 µg mL−1 min−1, 1.92 s−1, and 7.383 s−1 mg−1, respectively. The deactivation constant, half‐life, and change in Gibb's free energy, enthalpy, and entropy are determined at 50–70 °C, which supports the thermal stability and substrate affinity of the amylase. The washing quality of commercial detergents is distinctly enhanced when supplemented with this enzyme. Therefore, this amylase possesses significant thermal and surfactant stability has significant commercial potential.

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“…The study therefore suggests that the potential microbial sources from the extreme niches could provide hotbed for robust enzymes. [32][33][34][35][36]…”

Section: Application Of the Amylasementioning

confidence: 99%