Comparative Analysis of the Basement Membrane Composition of the Human Limbus Epithelium and Amniotic Membrane Epithelium

Dietrich-Ntoukas, Tina; Hofmann-Rummelt, Carmen; Kruse, Friedrich E.; Schlötzer‐Schrehardt, Ursula

doi:10.1097/ico.0b013e3182254b78

Cited by 43 publications

(23 citation statements)

References 40 publications

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“…As described above, several labs have demonstrated perlecan’s presence in the basement membrane of the normal corneal epithelium (Dietrich-Ntoukas et al, 2012; Schlötzer-Schrehardt et al, 2007), separating the epithelial and stromal layers, and in Descemet’s membrane, separating the stromal and endothelial layers. Its expression varies with age (Kabosova et al, 2007), and across various locations (Ljubimov et al, 1995), and is known to be expressed by corneal stromal cells, both normally and in response to injury (Brown et al, 1999; Hassell et al, 1992).…”

Section: Loss Of Perlecan At Tissue Borders Associated With Patholmentioning

confidence: 89%

“…Researchers may instinctively employ biologically-derived substrates to support cell proliferation and organization. For example, amniotic membrane is frequently employed, as it contains many basement membrane components, can be easily harvested and preserved, and may be sufficiently anti-inflammatory and anti-immunogenic for this use (Ahn et al, 2007; Dietrich-Ntoukas et al, 2012). However, the chief disadvantage of such biologically-derived membranes is their inherent variability, which may vary with the site of harvest, the age of the donor, post-processing methods, and the duration of pregnancy at the time of harvest.…”

Section: Translating Boundary Functions Into Useful Constructs Formentioning

confidence: 99%

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Border patrol: Insights into the unique role of perlecan/heparan sulfate proteoglycan 2 at cell and tissue borders

et al. 2014

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The extracellular matrix proteoglycan (ECM) perlecan, also known as heparan sulfate proteoglycan 2 or HSPG2, is one of the largest (>200 nm) and oldest (>550M years) extracellular matrix molecules. In vertebrates, perlecan’s five-domain structure contains numerous independently folding modules with sequence similarities to other ECM proteins, all connected like cars into one long, diverse complex train following a unique N-terminal domain I decorated with three long glycosaminoglycan chains, and an additional glycosaminoglycan attachment site in the C-terminal domain V. In lower invertebrates, perlecan is not typically a proteoglycan, possessing the majority of the core protein modules, but lacking domain I where the attachment sites for glycosaminoglycan chains are located. This suggests that uniting the heparan sulfate binding growth factor functions of domain I and the core protein functions of the rest of the molecule in domains II-V occurred later in evolution for a new functional purpose. In this review, we surveyed several decades of pertinent literature to ask a fundamental question: Why did nature design this protein uniquely as an extraordinarily long multifunctional proteoglycan with a single promoter regulating expression, rather than separating these functions into individual proteins that could be independently regulated? We arrived at the conclusion that the concentration of perlecan at functional borders separating tissues and tissue layers is an ancient key function of the core protein. The addition of the heparan sulfate chains in domain I likely occurred as an additional means of binding the core protein to other ECM proteins in territorial matrices and basement membranes, and as a means to reserve growth factors in an on-site depot to assist with rapid repair of those borders when compromised, such as would occur during wounding. We propose a function for perlecan that extends its role from that of an extracellular scaffold, as we previously suggested, to that of a critical agent for establishing and patrolling tissue borders in complex tissues in metazoans. We also propose that understanding these unique functions of the individual portions of the perlecan molecule can provide new insights and tools for engineering of complex multi-layered tissues including providing the necessary cues for establishing neotissue borders.

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Section: Loss Of Perlecan At Tissue Borders Associated With Patholmentioning

confidence: 89%

Section: Translating Boundary Functions Into Useful Constructs Formentioning

confidence: 99%

Border patrol: Insights into the unique role of perlecan/heparan sulfate proteoglycan 2 at cell and tissue borders

et al. 2014

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“…This is because the amniotic membrane is a soft semitransparent membrane without the corneal unique histoarchitecture. 31 Recent studies showed that a collagen scaffold constructed with porcine collagen was well-tolerated after heterogeneous implantation, and the porcine cornea shared similar anatomic characteristics with the human cornea. [32][33][34] Our previous research also indicated that the acellular porcine cornea matrix, which was developed by 0.5% sodium dodecyl sulfate (SDS) could preserve the key features of the cornea, including the intact basement membrane, and support the growth of CECs and keratocytes in vitro.…”

Section: Introductionmentioning

confidence: 98%

Reconstruction of Functional Ocular Surface by Acellular Porcine Cornea Matrix Scaffold and Limbal Stem Cells Derived from Human Embryonic Stem Cells

Zhu

Zhang

Sun

et al. 2013

Tissue Engineering Part A

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Limbal stem cells (LSCs) derived from human embryonic stem cells (hESCs) hold great potential for cell-based therapies for ocular surface diseases. The cell-based therapies mainly depend on an appropriate differentiation proposal with a high efficiency and on a suitable scaffold. In this study, we aimed to establish a feasible and efficient strategy for inducing hESCs into LSC-like cells by the LSC conditioned medium. The induced cells possessed the similar morphologic characteristics and expression of normal LSCs and showed a strong clonogenic and proliferative capacity in vitro. To construct a tissue-engineering corneal graft, these differentiated cells were seeded on an acelluar porcine corneal matrix (APCM), which maintained the corneal basement membrane in vitro. After 14 days culture, these induced cells gave rise to stratified epithelial cell sheets on the APCM and the basal cells still kept LSC characteristics. In rabbit total limbal stem cell deficiency (LSCD) models, the tissue-engineering graft had the potential to reconstruct the damaged ocular surface and alleviated the invasion of corneal neovascularization. These findings indicated that the engraftment constructed with the APCM scaffold and hESC-derived LSCs might be a potential therapy option for ocular surface regeneration in LSCD cases.

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“…To confirm that the lack of tyrosine sulfate recognition by PSG2 of the TM clone was due to the fact that only three tyrosine sulfate residues (192, 196, and 198) existed in Fbln2, all independent mutant clones along with WT and vector were transiently transfected into HEK293T, and cells were grown in the presence of Na 2 35 SO 4 . Media from these clones were immunoprecipitated with an anti-Myc antibody.…”

Section: Journal Of Biological Chemistry 13425mentioning

confidence: 99%

“…Fibulins are members of a seven-member family of extracellular matrix (ECM) 3 proteins that are important constituents of elastic fibers and basement membranes (1)(2)(3)(4)(5). They are divided into two classes based on their size (6).…”

mentioning

confidence: 99%

Fibulin 2, a Tyrosine O-Sulfated Protein, Is Up-regulated Following Retinal Detachment

Kanan

Brobst

Han

et al. 2014

Journal of Biological Chemistry

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Comparative Analysis of the Basement Membrane Composition of the Human Limbus Epithelium and Amniotic Membrane Epithelium

Cited by 43 publications

References 40 publications

Border patrol: Insights into the unique role of perlecan/heparan sulfate proteoglycan 2 at cell and tissue borders

Border patrol: Insights into the unique role of perlecan/heparan sulfate proteoglycan 2 at cell and tissue borders

Reconstruction of Functional Ocular Surface by Acellular Porcine Cornea Matrix Scaffold and Limbal Stem Cells Derived from Human Embryonic Stem Cells

Fibulin 2, a Tyrosine O-Sulfated Protein, Is Up-regulated Following Retinal Detachment

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