“…Several studies have shown the influence of these variables on the removal of C and N, biofilm morphology and composition, as well as on the diversity of total Bacteria and nitrifying bacteria communities developed in the biofilm [35][36][37][38]. In reference to the influence of the CFR, previous studies [35,37] have confirmed that this is one of the main factors explaining the variations in diversity and composition of biofilm total bacteria community and biofilm nitrifying bacteria. According to Wang et al [36], an increase in CFR promotes the increase of particle-particle and particle-wall collisions, which made the biofilm denser and thinner.…”