Comparative Analysis of Sugar Metabolites and Their Transporters in Sugarcane Following Sugarcane mosaic virus (SCMV) Infection

Abstract

Sugarcane mosaic virus (SCMV) is one of the major pathogens of sugarcane. SCMV infection causes dynamic changes in plant cells, including decreased photosynthetic rate, respiration, and sugar metabolism. To understand the basics of pathogenicity mechanism, we performed transcriptome and proteomics analysis in two sugarcane genotypes (Badila: susceptible to SCMV and B-48: SCMV resistant). Using Saccharum spontaneum L. genome as a reference, we identified the differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) that participate in sugar metabolism, transport of their metabolites, and Carbohydrate Activating enZYmes (CAZymes). Sequencing data revealed 287 DEGs directly or indirectly involved in sugar metabolism, transport, and storage, while 323 DEGs are associated with CAZymes. Significant upregulation of glucose, sucrose, fructose, starch, and SWEET-related transcripts was observed in the Badila after infection of SCMV. B-48 showed resistance against SCMV with a limited number of sugar transcripts up-regulation at the post-infection stage. For CAZymes, only glycosyltransferase (GT)1 and glycosyl hydrolase (GH)17 were upregulated in B-48. Regulation of DEGs was analyzed at the proteomics level as well. Starch, fructose, glucose, GT1, and GH17 transcripts were expressed at the post-translational level. We verified our transcriptomic results with proteomics and qPCR data. Comprehensively, this study proved that Badila upregulated sugar metabolizing and transporting transcripts and proteins, which enhance virus multiplication and infectionl.

Niche differentiation modulates metabolites abundance and composition in silicon fertilizer amended soil during sugarcane growth

“…In higher plants, studies have revealed that phenylpropanoid metabolic pathway is one of the main secondary metabolic pathways, consisting of many products, such as flavonoids, hydroxycinnamate amides and lignin, which are cardinal to plant growth and development [ 18 ]. Significant progress has been made by the sugarcane research community to identify and characterize the function of the bioactive components in sugarcane [ 19 , 20 ]. For example, Ezz et al [ 21 ] reported that 42 metabolites including nine fatty acids, nine flavonoids and two sterols were detected in sugarcane juice and molasses.…”

Compartment-dependent influence of sugarcane and soil on niche differentiation in metabolites, while silicon fertilizer mediates edaphic factors and sugarcane agronomic traits

“…In higher plants, studies have revealed that phenylpropanoid metabolic pathway is one of the main secondary metabolic pathways, consisting of many products, such as avonoids, hydroxycinnamate amides and lignin, which are cardinal to plant growth and development [18]. Signi cant progress has been made by the sugarcane research community to identify and characterize the function of the bioactive components in sugarcane [19][20]. For example, Ezz et al.…”

Improved Understanding of Sugar Transport in Various Plants

“…As pathogens rely on carbon source supply from hosts, sugar transporters have been reported to play a critical role in the process of feeding pathogens [ 1 , 7 ]. Akbar et al compared the expression of genes associated with sugar metabolism, sugar transport, and sugar storage, and compared related protein accumulation between two sugarcane genotypes infected with the Sugarcane mosaic virus (SCMV), one type susceptible to SCMV (Badila) and the other resistant to SCMV (B-48) [ 8 ]. They found that fewer those genes and proteins were regulated in the SCMV-resistant cultivar compared to the SCMV-susceptible cultivar [ 8 ].…”

“…Akbar et al compared the expression of genes associated with sugar metabolism, sugar transport, and sugar storage, and compared related protein accumulation between two sugarcane genotypes infected with the Sugarcane mosaic virus (SCMV), one type susceptible to SCMV (Badila) and the other resistant to SCMV (B-48) [ 8 ]. They found that fewer those genes and proteins were regulated in the SCMV-resistant cultivar compared to the SCMV-susceptible cultivar [ 8 ]. Specifically, glycosyl hydrolase 17, which is involved in plant immune responses, was highly upregulated in transcript and protein levels in the B-48 sugarcane, suggesting which might contribute to B-48’s SCMV resistance [ 8 ].…”

“…They found that fewer those genes and proteins were regulated in the SCMV-resistant cultivar compared to the SCMV-susceptible cultivar [ 8 ]. Specifically, glycosyl hydrolase 17, which is involved in plant immune responses, was highly upregulated in transcript and protein levels in the B-48 sugarcane, suggesting which might contribute to B-48’s SCMV resistance [ 8 ]. Although more research is needed to elucidate the molecular function of individual regulated genes, including sugar transporters, in SCMV resistance, this study provides insight into potentially important genes involved in SCMV resistance.…”