Comparative Analysis of Spectral Methods for Determining Bacteriochlorophyll d Concentration in Green Sulfur Bacteria in Water

Zhiltsova, A.A.; Filippova, O. A.; Krasnova, E. D.; Voronov, Dmitry A.; Patsaeva, Svetlana V.

doi:10.1134/s1024856022050232

Cited by 2 publications

(1 citation statement)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentration of Bchl (d+e), the main photosynthetic pigments of green sulfur bacteria, both green-colored and brown-colored, was determined from the absorption spectra of unfiltered water according to [42,43]. The area of the long-wavelength absorption band of BChl in the spectral range of 650-800 nm was calculated from the spectra, and then the Bchl concentration was calculated using the formula with an empirically determined coefficient.…”

Section: Water Sampling and Spectral Measurementsmentioning

confidence: 99%

Optical Proxies of Euxinia: Spectroscopic Studies of CDOM, Chlorophyll, and Bacteriochlorophylls in the Lagoon on Zeleny Cape (the White Sea)

et al. 2023

Self Cite

View full text Add to dashboard Cite

Along the shoreline of the White Sea, due to the post-glacial uplift of the coast, some water bodies with stable stratification have been formed. They have been classified as meromictic as they are at different stages of isolation from the Sea. As separation progresses, significant changes occur in the water column, including the composition of chromophoric dissolved organic matter (CDOM) and the structure of the aquatic microbial community. In this work, we searched for optical proxies of euxinia (anoxic conditions with accumulated hydrogen sulfide) in the water column of the meromictic lagoon on Zeleny Cape. The lagoon is separated from the White Sea basin by a shallow threshold that completely isolates the lagoon during low tide, but marine water enters the lagoon during high tide. The ecosystem in the lagoon is characterized by the marine salinity of water and a high organic matter content in the bottom water and sediments. In this study, spectral methods were used to obtain the depth distribution of CDOM, chlorophyll, and bacteriochlorophyll in the lagoon with strong water stratification and euxinic conditions in the bottom water. The measured optical CDOM characteristics were compared with hydrochemical data (water salinity, Eh, pH, dissolved oxygen), phytoplankton (oxygenic phototrophs), and green sulfur bacteria (anoxygenic phototrophs) distribution along the water column. The spectroscopic methods showed to have the advantages of not requiring water sample pre-treatment and allowing rapid sensing of CDOM and photosynthetic pigments at each horizon.

show abstract

Section: Water Sampling and Spectral Measurementsmentioning

confidence: 99%

Optical Proxies of Euxinia: Spectroscopic Studies of CDOM, Chlorophyll, and Bacteriochlorophylls in the Lagoon on Zeleny Cape (the White Sea)

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

Anoxygenic Phototrophic Bacteria of the Meromictic Lake Bol’shie Khruslomeny (Oleniy Island, Kandalaksha Gulf, Murmansk Oblast, Russia)

Lunina,

Grouzdev,

Patsaeva

et al. 2023

Microbiology

View full text Add to dashboard Cite

Abstract—The composition and structure of the anoxygenic phototrophic bacterial (APB) community in the water column of Lake Bol’shie Khruslomeny during winter were investigated. The community developed at the depth of 4.25 m, and its activity during the ice-covered period was very low (6.2 µmol C L–1 day–1). The water in the zone of highest development of phototrophic bacteria was of an unusual lemon-yellow color, probably due to the production and accumulation of polysulfides. The near-bottom water was also of lemon-yellow color and was resistant to oxidation by the air oxygen. In the zone of peak APB development the content of BChl d from green-colored green sulfur bacteria was considerably higher than that of BChl e from brown-colored green sulfur bacteria: 77 and 23%, respectively. The cultures of green and purple sulfur bacteria were isolated from lake water, and their physiological and genetic characteristics were determined. Two strains of green sulfur bacteria (brown-colored BrKhr17 and green-colored GrKhr17) contained gas vacuoles in their cells. Phylogenetically they were most closely related to the green-colored strain Chlorobium phaeovibrioides DSM 265 and were identified as new Chlorobium phaeovibrioides strains.

show abstract

Comparative Analysis of Spectral Methods for Determining Bacteriochlorophyll d Concentration in Green Sulfur Bacteria in Water

Cited by 2 publications

References 18 publications

Optical Proxies of Euxinia: Spectroscopic Studies of CDOM, Chlorophyll, and Bacteriochlorophylls in the Lagoon on Zeleny Cape (the White Sea)

Optical Proxies of Euxinia: Spectroscopic Studies of CDOM, Chlorophyll, and Bacteriochlorophylls in the Lagoon on Zeleny Cape (the White Sea)

Anoxygenic Phototrophic Bacteria of the Meromictic Lake Bol’shie Khruslomeny (Oleniy Island, Kandalaksha Gulf, Murmansk Oblast, Russia)

Contact Info

Product

Resources

About