Comparative analysis of specificity and sensitivity between Cobas 6800 system and SARS-CoV-2 rRT-PCR to detect COVID-19 infection in clinical samples

Grewal, Simmi; Gurcoo, Mehreen Syed; Sharma, Sarita

doi:10.1007/s00203-022-03118-y

Cited by 2 publications

(1 citation statement)

References 14 publications

(20 reference statements)

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“…It was noted that Cobas 6800 even detected eight patients (total 188) who had been stated negative by RT-PCR. The false negative result of RT-PCR occurred because the RdRp and N genes of SARS-CoV-2 remained undetected [ 37 ]. A study on diagnostic effectiveness stated that RT-PCR had an 84% positive predictive value (PPV) for positive tests and 32.4% false-positive reports [ 38 ].…”

Section: Sars-cov-2 Diagnostic Techniques and Their Clinical Relevancementioning

confidence: 99%

Conventional and Novel Diagnostic Tools for the Diagnosis of Emerging SARS-CoV-2 Variants

et al. 2023

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Accurate identification at an early stage of infection is critical for effective care of any infectious disease. The “coronavirus disease 2019 (COVID-19)” outbreak, caused by the virus “Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)”, corresponds to the current and global pandemic, characterized by several developing variants, many of which are classified as variants of concern (VOCs) by the “World Health Organization (WHO, Geneva, Switzerland)”. The primary diagnosis of infection is made using either the molecular technique of RT-PCR, which detects parts of the viral genome’s RNA, or immunodiagnostic procedures, which identify viral proteins or antibodies generated by the host. As the demand for the RT-PCR test grew fast, several inexperienced producers joined the market with innovative kits, and an increasing number of laboratories joined the diagnostic field, rendering the test results increasingly prone to mistakes. It is difficult to determine how the outcomes of one unnoticed result could influence decisions about patient quarantine and social isolation, particularly when the patients themselves are health care providers. The development of point-of-care testing helps in the rapid in-field diagnosis of the disease, and such testing can also be used as a bedside monitor for mapping the progression of the disease in critical patients. In this review, we have provided the readers with available molecular diagnostic techniques and their pitfalls in detecting emerging VOCs of SARS-CoV-2, and lastly, we have discussed AI-ML- and nanotechnology-based smart diagnostic techniques for SARS-CoV-2 detection.

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Section: Sars-cov-2 Diagnostic Techniques and Their Clinical Relevancementioning

confidence: 99%

Conventional and Novel Diagnostic Tools for the Diagnosis of Emerging SARS-CoV-2 Variants

et al. 2023

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Evaluation of SARS-CoV-2 identification methods through surveillance of companion animals in SARS-CoV-2-positive homes in North Carolina, March to December 2020

Gin,

Petzold,

Uthappa

et al. 2023

PeerJ

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We collected oral and/or rectal swabs and serum from dogs and cats living in homes with SARS-CoV-2-PCR-positive persons for SARS-CoV-2 PCR and serology testing. Pre-COVID-19 serum samples from dogs and cats were used as negative controls, and samples were tested in duplicate at different timepoints. Raw ELISA results scrutinized relative to known negative samples suggested that cut-offs for IgG seropositivity may require adjustment relative to previously proposed values, while proposed cut-offs for IgM require more extensive validation. A small number of pet dogs (2/43, 4.7%) and one cat (1/21, 4.8%) were positive for SARS-CoV-2 RNA, and 28.6 and 37.5% of cats and dogs were positive for anti-SARS-CoV-2 IgG, respectively.

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Comparative analysis of specificity and sensitivity between Cobas 6800 system and SARS-CoV-2 rRT-PCR to detect COVID-19 infection in clinical samples

Cited by 2 publications

References 14 publications

Conventional and Novel Diagnostic Tools for the Diagnosis of Emerging SARS-CoV-2 Variants

Conventional and Novel Diagnostic Tools for the Diagnosis of Emerging SARS-CoV-2 Variants

Evaluation of SARS-CoV-2 identification methods through surveillance of companion animals in SARS-CoV-2-positive homes in North Carolina, March to December 2020

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