Comparative Analysis of Solasodine from &lt;i&gt;in vitro&lt;/i&gt; and &lt;i&gt;in vivo&lt;/i&gt; Cultures of &lt;i&gt;Solanum nigrum&lt;/i&gt; Linn.

Bhakyaraj, R.; Chanthuru, A.; Parvathi, S.; Palanivel, S.

doi:10.3126/kuset.v5i1.2850

Cited by 22 publications

(9 citation statements)

References 4 publications

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“…These findings are in synchrony with those of Janarthanam et al [18] and Rajkumar et al [19], who have obtained large amount of bioactive compound in undifferentiated callus culture than field grown plants. Similar studies have been conducted in Solanum nigrum [20], wherein the authors have reported that the solasodine content from the in vitro callus extracts was much higher than extracts of field grown leaves.…”

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confidence: 55%

Mucilage Synthesis in Callus Cultures of Plantago ovata Forsk

Gupta

Kour

Kaul

et al. 2014

Natl. Acad. Sci. Lett.

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The seed husk of Plantago ovata known as psyllium (Isabgol) yields medicinally important mucilage. The amount of mucilage produced is about 25 % (by weight) of the total seed yield. In the present study, an attempt was made to increase the amount of mucilage through callus cultures of P. ovata. The first step involved establishment of callus cultures in P. ovata. Leaf explants from 10 to 20 day old seedlings were cultured on MS basal medium supplemented with various concentrations of different plant growth regulators. The highest rate of callus induction (89 %) was obtained on MS medium containing 0.5 mg l -1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg l -1 Kinetin. The mucilage content was estimated from the callus obtained in different media. The best mucilage production was obtained in the MS medium supplemented with 0.25 mg l -1 2,4-D and 0.25 mg l -1 thidiazuron. Significant differences with regard to the total mucilage content were recorded. Overall, the callus produced nearly five times more mucilage than the seeds. The present technology provides an alternative route to production of large quantities of mucilage without plants.The seed husk of Plantago ovata Forsk, is an effective laxative. Seeds of P. ovata release mucilage in water, which is an anionic polysaccharide of L-arabinose, D-xylose and Dgalacturonic acid [1], and is used to treat diverticulitis [2], constipation, diarrhoea, haemorrhoids, bladder problems, irritable bowel syndrome, high blood pressure, high cholesterol and type 2 diabetes [3]. Mucilage of plant origin is in high demand nowadays, and it is very difficult to fulfil the entire demand by field grown plants only. For this purpose, a striking and very promising alternative system for commercial exploitation is the plant tissue culture by using cell suspension culture systems [4]. However, so far any attempts to increase mucilage have not yielded positive results in P. ovata. Studies along these lines have been conducted in Plantago lanceolata where in media having different combinations of plant growth regulators (PGRs) have been used to obtain mucilage from callus [5]. It has been reported that callus cultures are relatively rich in mucilage which commonly make up between 8-10 and 0.2-3.7 % of dry weight in some plants [6,7]. To the best of our knowledge, there are no reports on in vitro mucilage production from the callus cultures of P. ovata. Therefore, the present study was aimed to explore the possibility of mucilage production through in vitro culture.Seeds of P. ovata were available in the collection center of School of Biotechnology, University of Jammu, Jammu, India. The seeds were sterilized first with 70 % V/V ethanol for 1 min, then with 5 % w/v sodium-hypochlorite solution for 20 min, finally washed five times with sterile distilled water. The sterilized seeds were aseptically germinated on an agar-solidified MS basal medium [8] and incubated at a temperature of 25 ± 1°C. Leaf segments (1.5 cm) were excised from 10 to 20 day-old seedlings and were used as explants ...

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confidence: 55%

Mucilage Synthesis in Callus Cultures of Plantago ovata Forsk

Gupta

Kour

Kaul

et al. 2014

Natl. Acad. Sci. Lett.

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“…Results of earlier experiments revealed that total glycoalkaloid content per dry weight was less than 0.2% in the roots when this plant was grown in natural conditions. Several papers have described the production of solasodine, a type of glycoalkaloid, in tissue cultures of Solanum laciniatum (Macek 1989) and Solanum nigrum (Yogananth et al 2009). However, glycoalkaloid production in cultured tissue of Solanum species has not received much attention, and there have been a limited number of reports relating to the tissue culturing of S. hainanense (Anh et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Production of glycoalkaloids from callus cultures of Solanum hainanense Hance

Lộc¹,

Anh²,

Binh³

et al. 2010

Journal of Plant Biotechnology

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Leaf explants of the Solanum hainanense plant, grown in vitro, were cultured in basal Murashige and Skoog (MS) media supplemented with 0.5 mg/L kinetin and 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) for callus initiation. For maintenance and proliferation, the callus was cultured on MS medium supplemented with 1 mg/L 6-benzylaminopurine (BAP) and 0.5 mg/L 2,4-D. The glycoalkaloid content in the callus was at its maximum after ten weeks of culture (188.65 mg/g), whereas that of the one-year-old control was 22.22 mg/g in the root and 5.99 mg/g in the stem. The glycoalkaloid extracted from the callus inhibited the activity of collagenase on collagen gel. High performance liquid chromatography (HPLC) analysis showed that biotransformation occurred when a callus was grown on medium supplemented with various carbon sources. These results suggest that callus of S. hainanense is a good material for production of glycoalkaloid.

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“…Berberine alkaloid was produced from in vitro culture of Thalictrum minor 1 000 folds than original plant [13]. In vitro production of solasodine from cultures of S. nigrum (0.142 mg/g) in higher yields than parent plant (0.079 8 mg/ g) which was equal to 1.78 folds was also reported [15]. High yield of glycoalkaloids of solanidine series from Solanum tuberosum culture, reached 1.44 and 3.88 folds of the concentration of mother plant from calli and shoots, respectively were reported from Solanum tuberosum cultures [16].…”

Section: Table 11mentioning

confidence: 92%

“…The challenge of producing natural antibilharziasis drugs from plant origin lead to searching for alternative way rather than breeding for producing such valuable compounds. Different studies of in vitro glycoalkaloids production from Solanum species are reported [13][14][15][16].…”

Section: A Rt I C L E I N F O Abstractmentioning

confidence: 99%