Comparative analysis of signaling pathways in peripheral blood from patients with Kashin-Beck disease and osteoarthritis

Abstract

Abstract. The aim of the present study was to investigate the early diagnostic biomarkers of Kashin-Beck disease (KBD), and to compare the common signaling pathways of peripheral mononuclear cells between patients with KBD and those with osteoarthritis (OA). A total of 20 and 12 peripheral blood samples were separately collected from KBD patients and normal control subjects, respectively, in an endemic area according to the diagnosis criteria. Total RNAs were extracted and gene expression levels were determined using an Agilent whole genome expression microarrays. The gene expression data of OA were obtained from GEO published database. Significant different pathways between KBD and OA were analyzed using Ingenuity Pathway Analysis software. A total of 82 differentially expressed genes, 51 significant different signaling pathways and five significant biological functions were identified in KBD patient samples, while 89, 50 and five significantly different genes, pathways and functions were identified in OA. Nine common significant pathways and five common differentially expressed genes were identified between the KBD and OA. Nine common significant pathways and five common differentially expressed genes were found between the two diseases. The present results suggest that there are similarities in vascular microcirculation, immunoreactions and cell apoptosis between KBD and OA, which may contribute to the early diagnosis and pathogenetic study of KBD.

Deciphering the Association of Cytokines, Chemokines, and Growth Factors in Chondrogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells Using an ex vivo Osteochondral Culture System

“…Fischer's test in IPA helps to identify the bio functions and canonical pathways that are significantly associated with the cytokines of interest to the disease. Most of the identified pathways following stem cells' interaction in the management of OA were in line with an earlier study report (Ning et al, 2016), which included cytokines, p38 MAPK, and TNF receptors signaling (Ning et al, 2016).…”

Screening for differentially expressed circRNA between Kashin–Beck disease and osteoarthritis patients based on circRNA chips

“…Because of the similar clinical manifestations between KBD and OA, KBD is known as an endemic OA. In recent years, circRNA has been proved to be a new biomarker in human peripheral blood [21] for diagnosis of diseases, such as coronary artery disease, OA and rheumatoid arthritis (RA) [22][23][24]. Until now, there have been no identification and characterization of circRNAs for OA and KBD in bone or cartilage tissue as well as in peripheral blood.…”