Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs, and SRAPs

Abstract

Buffalograss [ Buchloe dactyloides (Nutt.) Englem.] is the only native grass that is being used extensively as a turfgrass in the Great Plains region. Its low-growth habit, drought resistance, and low-maintenance requirement make it attractive as a turfgrass species. Our objective was to obtain an overview on the genetic relatedness among and within seeded and vegetative biotype buffalograsses using inter-simple sequence repeats (ISSRs), random amplified polymorphic DNA (RAPDs), sequence-related amplified polymorphisms (SRAPs), and simple sequence repeats (SSRs) markers that were derived from related species (maize, pearl millet, sorghum, and sugarcane). Twenty individuals per cultivar were genotyped using 30 markers from each marker system. All buffalograss cultivars were uniquely fingerprinted by all four marker systems. Mean genetic similarities were estimated at 0.52, 0.51, 0.62, and 0.57 using SSRs, ISSRs, SRAPs, and RAPDs, respectively. Two main clusters separating the seeded-biotype from the vegetative-biotype cultivars were produced using UPGMA analysis. Further subgroupings were unequivocal. The Mantel test resulted in a very good fit (SRAP=0.92, ISSR=0.90) to good fit (RAPD=0.86, SSR=0.88) of cophenetic values. Comparing the four marker systems to each other, RAPD and SRAP similarity indices were highly correlated ( r=0.73), while Spearman's rank correlation coefficient between RAPDs and SSRs was r=0.24 and between ISSRs and SSRs was r=0.66. A genotype-assignment analytical approach might be useful for cultivar identification and property rights protection. Polymorphic SRAPs were abundant and demonstrated genetic diversity among closely related cultivars.

Genetic Diversity among Wild Resources of the GenusBoehmeriaJacq. from West China Determined Using Inter-simple Sequence Repeat and Rapid Amplification of Polymorphic DNA Markers

“…A higher proportion of polymorphic bands were observed using the ISSR (96.3%) method than RAPD (95.5%) method, as suggested by some authors (Bahattin, 2003;Budak et al, 2004;Marotti et al, 2007).…”

The trnL-F plastid DNA characters of three Poa pratensis (Kentucky bluegrass) varieties

“…Plastid sequence characters are much less likely to be meaningful in strictly outbreeding grasses like ryegrass (Dinelli and Lucchese 1999), creeping bentgrass (Caceres et al 2000), and tall fescue (Xu et al 1994) or dioecious grasses like buffalograss (Budak et al 2004). In these cases, each cultivar is a heterogenous population of genotypes, and it is highly unlikely that different cultivars each have a single, separate maternal ancestor.…”

Molecular diversity and population structure of the forage grass Hemarthria compressa (Poaceae) in south China based on SRAP markers

“…In addition, the cost of SRAP is less than with amplified fragment length polymorphism (AFLP). Up to now, SRAP has been successfully used for the evaluation of genetic diversity (Ferriol et al, 2003;Budak et al, 2004;Comlekcioglu et al, 2010), genetic structure (Wang et al, 2004;Xie et al, 2005;Zhang et al, 2005), and map construction (Lin et al, 2003;Suenaga et al, 2005), among others. There is no report yet about the use of SRAP markers in Hemarthria compressa.…”