Comparative analysis of SAF, Protaper Next and BT‐Race in eliminating Enterococcus faecalis from long oval canals: An ex vivo study

Abstract

Comparison of the ability of newly designed rotary files to eliminate viable Enterococcus faecalis populations from long oval root canals of extracted human teeth to that of the self-adjusting file (SAF). One hundred caries-free, single-rooted, long oval teeth were contaminated with E. faecalis. The teeth were randomly distributed into four groups (n = 25) as follows: G.1, manual; G.2, SAF; G.3, ProTaper Next; and G.4, BT-Race. Two microbial samples were obtained from each tooth with sterile paper points, (s1) before and (s2) after instrumentation. The relative reduction in colony-forming units (CFUs) from s1 to s2 measurements was calculated and compared among the groups using parametric Kruskal-Wallis one-way anova on ranks and Dunn's method (a = 0.05). The results indicated a descending order of the groups with regard to efficacy as follows: BT-Race, Next, SAF and manual. The statistical analysis showed that the relative percentage reduction (RR) of CFUs was lower in the manual group than in the other groups, while the SAF group showed a significantly lower RR than the BT-Race group (P < 0.05). The efficacy in reduction of the microbiological load of viable E. faecalis from long oval root canals was different between the tested endodontic systems.

Instrument Design May Influence Bacterial Reduction During Root Canal Preparation

“…The plate culture method was used in this study because of its simplicity and efficiency (5,7,(8)(9)(10)(11)(13)(14)(15)(16)(17)(18)(19). Molecular methods has been highlighted because of their high sensibility to detect microorganisms, but such a feature would not be necessary in this study as there was no total absence of bacterial growth in the collection.…”

Rotary versus reciprocating shaping files in bacterial elimination from long oval canals

“…Residual NaOCl was deactivated with 10 mL of 10% sodium thiosulphate (Na 2 O 3 S 2 ,) and then rinsed with 5 mL of distilled water. After the initial preparation, the teeth were immersed in trypticase soy broth (TSB), placed in an ultrasonic bath for 1 min to release entrapped air and allow penetration of the culture media into the root irregularities and then sterilised in an autoclave for 20 min at 121°C .…”

“…An E. faecalis strain (ATCC 29212) was used to infect the root canals . A suspension was prepared by adding 1 mL of a pure E. faecalis culture grown in TSB for 24 h to 5 mL of fresh TSB.…”

“…Our findings showed that the BT‐RaCe demonstrated cleaning ability that was significantly superior to that of the other systems . In this study, using the same method, we compared the BT‐RaCe and WaveOne systems and their ability to clean viable E. faecalis populations from long oval root canals of extracted human teeth.…”

“…Our previous study compared the SAF with two newly designed rotary file systems, ProTaper Next (Maillefer, Ballaigues, Switzerland) and BT‐RaCe (FKG Dentaire, LaSaux‐de‐Fonds, Switzerland), assessing the ability to eliminate viable Enterococcus faecalis populations from long oval root canals in extracted human teeth .…”