Comparative analysis of root transcriptome profiles between drought-tolerant and susceptible wheat genotypes in response to water stress

Hu, Ling; Xie, Yan; Fan, Shoujin; Wang, Zongshuai; Wang, Fahong; Zhang, Bin; Li, Haosheng; Song, Jie; Kong, Lingan

doi:10.1016/j.plantsci.2018.03.036

Cited by 83 publications

(66 citation statements)

References 112 publications

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“…A total of 50,981, 51,140, 52,622 and 52,961 genes were expressed in JM_I, JM_R and YZ_I and YZ_R, respectively. This means that compared to irrigation condition, more genes were induced to express by drought stress in both drought-tolerant and sensitive genotypes, which is consistent with previous studies [20,36]. From the point of view of the genotype, the variety YZ has more abundant expressed genes than the JM variety.…”

Section: Rna-seq Analysissupporting

confidence: 90%

“…In addition, the wheat MYB gene TaMYBsdu1 is found to be up-regulated under drought stress but showed differential expression between tolerant and sensitive genotypes, suggesting it plays a crucial role in regulating drought tolerance [34]. With the advent of high-throughput sequencing technology, RNA deep sequencing has been widely used to investigate the gene differential expression profiles involved in drought response in wheat at the transcriptome level, which provided a direct and effective method to identify drought-inducible genes and also contribute to better understanding of drought-signaling pathways [35,36]. It has been demonstrated that different wheat cultivars had diverse molecular basis for drought response and adaptation and the performance of wheat under controlled conditions showed less correlation with field performance [20,37,38].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Analysis of the Transcriptional Response of Tolerant and Sensitive Wheat Genotypes to Drought Stress in Field Conditions

Feng

Peng

et al. 2018

Agronomy

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Drought stress is one of the most adverse environmental limiting factors for wheat (Triticum aestivum L.) productivity worldwide. For better understanding of the molecular mechanism of wheat in response to drought, a comparative transcriptome approach was applied to investigate the gene expression change of two wheat cultivars, Jimai No. 47 (drought-tolerant) and Yanzhan No. 4110 (drought-sensitive) in the field under irrigated and drought-stressed conditions. A total of 3754 and 2325 differential expressed genes (DEGs) were found in Jimai No. 47 and Yanzhan No. 4110, respectively, of which 377 genes were overlapped, which could be considered to be the potential drought-responsive genes. GO (Gene Ontology) analysis showed that these DEGs of tolerant genotype were significantly enriched in signaling transduction and MAP (mitogen-activated protein) kinase activity, while that of sensitive genotype was involved in photosynthesis, membrane protein complex, and guard cell differentiation. Furthermore, 32 and 2 RNA editing sites were identified in drought-tolerant and sensitive genotypes under drought compared to irrigation, demonstrating that RNA editing also plays an important role in response to drought in wheat. This study investigated the gene expression pattern and RNA editing sites of two wheat cultivars with contrasting tolerance in field condition, which will contribute to a better understanding of the molecular mechanism of drought tolerance in wheat and beyond.

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Section: Rna-seq Analysissupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of the Transcriptional Response of Tolerant and Sensitive Wheat Genotypes to Drought Stress in Field Conditions

Feng

Peng

et al. 2018

Agronomy

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“…Comparatively, the antioxidant enzymes activity was relatively higher in PI 585453 (SOD), PI 585451 (CAT) and PI 585454 (POD) than other genotypes. Consistent with our findings, higher enzyme antioxidant activities have been reported in drought-tolerant wheat genotypes than that in the sensitive type [32,33], indicating that the sorghum genotypes (PI 585453, PI 585452 and PI 585456) exhibited enhanced ROS scavenging system than other genotypes.…”

Section: The Biochemical Aspect Of Drought Response Among Sorghum Gensupporting

confidence: 91%

Comparative Physiological, Biochemical and Transcript Response to Drought in Sorghum Genotypes

Amoah

Antwi‐Berko

2020

BJI

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Sorghum [Sorghum bicolor (L.) Moench] is considered as an important staple crop in the tropical regions. However, the productivity of this useful crop is hindered by drought which contributes to significant yield reduction. The present study aimed to decipher the effects of drought stress on physiological, biochemical and gene expression changes in sorghum genotypes and to ascertain the differences in their response to drought stress. To achieve these objectives, six sorghum genotypes were grown in pot culture in a greenhouse, in a randomized complete block design and exposed to water stress treatment for 10 days. From the study, drought stress caused a significant (P < .05) reduction in plant height, leaf water and chlorophyll contents while the proline, malondialdehyde (MDA), soluble sugar, electrolyte leakage (EL), hydrogen peroxide (H2O2) and antioxidant enzymes activity increased significantly (P < .05) and differentially in all sorghum genotypes. Among the genotypes investigated, PI 585456 showed enhanced performance and was considered as the most tolerant to drought in relation to plant growth and water relation, membrane status, photosynthetic activity, ROS and osmolytes accumulation and antioxidant enzymes activity. Furthermore, the transcript expression analyses of different categories of drought-responsive genes, viz; antioxidant-related, osmolytes biosynthesis-related, dehydrin-related, photosystem-related and transcription-related were differentially upregulated in sorghum genotypes investigated. The results revealed the differences in metabolic response to drought among the genotypes, which accentuated the physiological, biochemical and molecular mechanism related to a specific response that may play a vital role in drought tolerance in sorghum.

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“…Under prolonged drought stress, the preservation of the root water content at a steady level is a crucial strategy for plant survival and growth. Increasing physiological and molecular evidence suggests that the water retention capacity of the root zone is maintained through osmotic adjustment and root waterproof barriers 4,30,38 . Based on the above findings, we inferred that O. taihangensis might have effective mechanisms to maintain its root water content.…”

Section: Discussionmentioning

confidence: 99%

“…Hence, plant root systems are critical components of plants to cope with drought stress and maintain production. In recent years, some studies have devoted efforts to root responses to drought at the genetic level in rice 29 , wheat 30 , sunflower 31 , soybean 32 , Ammopiptanthus mongolicus 33 , and grape 34 . O. taihangensis mainly survives on the cracks of steep slopes and cliffs at an altitude of approximately 1000 m in the Taihang Mountains ( Supplementary Fig.…”

mentioning

confidence: 99%

Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis

Yang

Guo

Zhong

et al. 2020

Sci Rep

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Opisthopappus taihangensis (Ling) Shih, as a relative of chrysanthemum, mainly survives on the cracks of steep slopes and cliffs. Due to the harsh environment in which O. taihangensis lives, it has evolved strong adaptive traits to drought stress. The root system first perceives soil water deficiency, triggering a multi-pronged response mechanism to maintain water potential; however, the drought tolerance mechanism of O. taihangensis roots remains unclear. Therefore, roots were selected as materials to explore the physiological and molecular responsive mechanisms. We found that the roots had a stronger water retention capacity than the leaves. This result was attributed to ABA accumulation, which promoted an increased accumulation of proline and trehalose to maintain cell osmotic pressure, activated SOD and POD to scavenge ROS to protect root cell membrane structure and induced suberin depositions to minimize water backflow to dry soil. Transcriptome sequencing analyses further confirmed that O. taihangensis strongly activated genes involved in the ABA signalling pathway, osmolyte metabolism, antioxidant enzyme activity and biosynthesis of suberin monomer. overall, these results not only will provide new insights into the drought response mechanisms of O. taihangensis but also will be helpful for future drought breeding programmes of chrysanthemum. Drought stress is one of the most common abiotic stresses that threatens the healthy growth and development of plants. With the further aggravation of global warming and shortages of fresh water associated with population growth, it is estimated that drought stress will severely reduce the yield and quality of crops and ornamental plants 1. Therefore, further exploration of the physiological and molecular mechanisms is necessary for breeding drought-tolerant plants. Plants have evolved physiological, biochemical and molecular strategies to adapt to arid environments and to prevent cells from water deficiency. Physiological adaptability, including abscisic acid (ABA) content changes, proline accumulation, and superoxide dismutase (SOD) and peroxidase (POD) enzyme activities, are fundamental for plants to withstand drought stress 2-5. Drought-responsive molecular mechanisms have been divided into two terms: those that directly protect plants against drought stress and those that regulate targeted gene expression and signal transduction in plants in response to drought 6. The first term includes genes encoding proteins that function by protecting cell turgor, such as enzymes participating in the biosynthesis of various osmoprotectants 4,7,8. Moreover, late-embryogenesis-abundant proteins, chaperones and antioxidant enzymes directly prevent plants from drought damage. The second term of genes mainly comprises transcription factors, which are activated by signal transduction pathways and regulate functional genes 2,9. Furthermore, protein kinases, protein phosphatases, enzymes related to phospholipid metabolism and ubiquitin ligase play significant roles in the signal trans...

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Comparative analysis of root transcriptome profiles between drought-tolerant and susceptible wheat genotypes in response to water stress

Cited by 83 publications

References 112 publications

Comparative Analysis of the Transcriptional Response of Tolerant and Sensitive Wheat Genotypes to Drought Stress in Field Conditions

Comparative Analysis of the Transcriptional Response of Tolerant and Sensitive Wheat Genotypes to Drought Stress in Field Conditions

Comparative Physiological, Biochemical and Transcript Response to Drought in Sorghum Genotypes

Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis

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