Comparative analysis of rhamnolipid congener synthesis in neotype Pseudomonas aeruginosa ATCC 10145 and two marine isolates

Du, Jin; Zhang, Aijun; Zhang, Xiaoqing; Si, Xiaoguang; Cao, Junrui

doi:10.1016/j.biortech.2019.121380

Cited by 12 publications

(6 citation statements)

References 43 publications

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“…Similar results were obtained by Lotfabad et al (2010). Du et al (2019) mentioned in their work that two marine isolates (1A01151 and 1A00364) showed production of rhamnolipids and the most abundant mono-and di-congeners were Rha-C 10 -C 10 and Rha-Rha-C 1 0 -C 1 0 . A similar report by Woźniak- Karczewska et al (2017) showed that an axenic strain of P. aeruginosa produced a rhamnolipid with Rha-C 10 -C 10 as the predominant congener.…”

Section: Discussionsupporting

confidence: 87%

Characterization of Enterobacter cloacae BAGM01 Producing a Thermostable and Alkaline-Tolerant Rhamnolipid Biosurfactant from the Gulf of Mexico

et al. 2020

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The search for novel biosurfactants (Bs) requires the isolation of microorganisms from different environments. The Gulf of Mexico (GoM) is a geographical area active in the exploration and exploitation of hydrocarbons. Recent metagenomic and microbiologic studies in this area suggested a potential richness for novel Bs microbial producers. In this work, nineteen bacterial consortia from the GoM were isolated at different depths of the water column and marine sediments. Bs production from four bacterial consortia was detected by the CTAB test and their capacity to reduce surface tension (ST), emulsion index (EI 24 ), and hemolytic activity. These bacterial consortia produced Bs in media supplemented with kerosene, diesel, or sucrose. Cultivable bacteria from these consortia were isolated and identified by bacterial polyphasic characterization. In some consortia, Enterobacter cloacae was the predominant specie. E. cloacae BAGM01 presented Bs activity in minimal medium and was selected to improve its Bs production using a Taguchi and Box-Behnken experimental design; this strain was able to grow and presented Bs activity at 35 g L −1 of NaCl. This Bs decreased ST to around 34.5 ± 0.56 mNm −1 and presented an EI 24 of 71 ± 1.27%. Other properties of this Bs were thermal stability, stability in alkaline conditions, and stability at high salinity, conferring important and desirable characteristics in multiple industries. The analysis of the genome of E. cloacae BAGM01 showed the presence of rhlAB genes that have been reported in the synthesis of rhamnolipids, and alkAB genes that are related to the degradation of alkanes. The bioactive molecule was identified as a rhamnolipid after HPLC derivatization, 1 H NMR, and UPLC-QTOF-MS analysis.

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Section: Discussionsupporting

confidence: 87%

Characterization of Enterobacter cloacae BAGM01 Producing a Thermostable and Alkaline-Tolerant Rhamnolipid Biosurfactant from the Gulf of Mexico

et al. 2020

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“…Glycolipids are well-known for relatively easy large-scale production and various applications as surfactants; in particular, the yeast-derived sophorolipids, MELs, and rhamnolipids ( 5 ) from P. aeruginosa ( 5 ). This bacterium is known to be ubiquitously distributed; hence, it is not surprising that it was also isolated from marine samples as a producer of rhamnolipids [90,91,92]. However, P. aeruginosa is also a well-known human pathogen, making it less attractive for industrial applications.…”

Section: Lmw Biosurfactants From Marine Sourcesmentioning

confidence: 99%

Marine Biosurfactants: Biosynthesis, Structural Diversity and Biotechnological Applications

et al. 2019

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Biosurfactants are amphiphilic secondary metabolites produced by microorganisms. Marine bacteria have recently emerged as a rich source for these natural products which exhibit surface-active properties, making them useful for diverse applications such as detergents, wetting and foaming agents, solubilisers, emulsifiers and dispersants. Although precise structural data are often lacking, the already available information deduced from biochemical analyses and genome sequences of marine microbes indicates a high structural diversity including a broad spectrum of fatty acid derivatives, lipoamino acids, lipopeptides and glycolipids. This review aims to summarise biosyntheses and structures with an emphasis on low molecular weight biosurfactants produced by marine microorganisms and describes various biotechnological applications with special emphasis on their role in the bioremediation of oil-contaminated environments. Furthermore, novel exploitation strategies are suggested in an attempt to extend the existing biosurfactant portfolio.

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“…The most abundant mono-(41.37%) and di-(58.63%) congeners were Rha-C10-C10 and Rha-Rha-C10-C10, respectively. Strain 1A01151 showed a decreased mono-congener proportion and an increased di-congener proportion compared to ATCC 10145, with differences in speci c congener proportions(Du et al 2019). In view of previous reports indicating the strain-dependent and culture condition-dependent variability in rhamnolipid congener composition.…”

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confidence: 58%

Soyabean Oil Concentration Dependent Abundance of Rhamnolipid Homologues Determines Microbial Enhanced Oil Recovery Efficacy In Pseudomonas aeruginosa RA5

Ankulkar

Aphale

Chavan³

2023

Preprint

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In the present research, we isolated rhamnolipid (RLs) biosurfactant producing Pseudomonas aeruginosa RA5 from oil contaminated refinery soil. The proportion of its major mono-RLs and di-RLs homologues; Rha-C10, Rha-C10-C10, Rha-Rha-C10, and Rha-Rha-C10-C10 was investigated with minor change in the Soyabean oil concentration from 2–4%. We report P. aeruginosa RA5 for its capacity to efficiently produce di-rhamnolipid (Rha-Rha-C10-C10) as the predominant component with 2% and 4% soybean oil as a sole carbon source, accounting for 3.7 % and 0.7% of total products, respectively. The critical micelle concentration (CMC) of rhamnolipid products varies with the content of di-rhamnolipid, whereby lower CMC values correspond to higher di-rhamnolipid contents. The rhamnolipids containing 3.7% di rhamnolipid had the lowest CMC value of 206 mg/L. Accordingly, the viscosity-reducing efficiency and oil-washing efficiency of rhamnolipids increased with the higher di-rhamnolipid component. At a concentration of 1000 mg/L, the rhamnolipids containing 3.7% di-rhamnolipid (Rha-Rha-C10-C10) worked best and showed 85% oil-washing efficiency, which offered great promise for applications in enhanced oil recovery. The results showed the variation of structure and composition of rhamnolipids had a significant effect on their application.

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Comparative analysis of rhamnolipid congener synthesis in neotype Pseudomonas aeruginosa ATCC 10145 and two marine isolates

Cited by 12 publications

References 43 publications

Characterization of Enterobacter cloacae BAGM01 Producing a Thermostable and Alkaline-Tolerant Rhamnolipid Biosurfactant from the Gulf of Mexico

Characterization of Enterobacter cloacae BAGM01 Producing a Thermostable and Alkaline-Tolerant Rhamnolipid Biosurfactant from the Gulf of Mexico

Marine Biosurfactants: Biosynthesis, Structural Diversity and Biotechnological Applications

Soyabean Oil Concentration Dependent Abundance of Rhamnolipid Homologues Determines Microbial Enhanced Oil Recovery Efficacy In Pseudomonas aeruginosa RA5

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