Comparative Analysis of RAPD and ISSR Markers for Characterization of Sesame (Sesamum indicum L) Genotypes

RAPD and ISSR Methods Used for Fingerprinting of Selected Accessions of Viburnum

“…The results prove that the Dendrological Garden in Przelewice is a rich collection of both cultivated and botanical accessions, as well as hybrids of Viburnum species of different origin. Similar results were observed for several other species, such as black gram (Souframanien and Gopalakrishna 2004), where the percentage of polymorphism ranged from 16.6 to 66.6%; sesame (Sharma et al, 2009), where 56.18 and 66.78% of polymorphic bands were amplified by both RAPD and ISSR primers, and Lobathallia radiosa (Yüzbaşioğlu et al, 2011), where all the obtained bands were polymorphic.…”

“…As a result of the amplifications with the use of the examined Viburnum genotypes, 1160 (RAPD) and 873 (ISSR) amplicons were scored as the effect of amplification of 690 RAPD and 418 ISSR loci, respectively. The length of the amplified products corresponded to the number of products amplified, the number of primers used, and the length of products mostly generated with these methods and presented in papers by Sharma et al (2009), Williams et al (1990), Yüzbaşioğlu et al (2011) and Zietkiewicz et al (1994). We demonstrated high genetic variability among the examined Viburnum objects.…”

“…As an extraordinary valuable source of Viburnum genes, it enables morphological, phenological and molecular analyses. It can be used for genotyping and description of mutual genetic relationships with the use of different methods, including those based on PCR amplification with randomly amplified polymorphic DNA (RAPD), as well as inter simple sequence repeats (ISSR) (Hyvönen, 2010;Poczai and Hyvönen, 2010;Sharma et al, 2009;Shneyer, 2009, Smolik andKrzysztoszek (2010)). The first one amplifies DNA using 10-mer primers, while the second one involves amplification of DNA sequence between two adjacent and inversely oriented microsatellites usually by using 18-24-mer primers (Williams et al, 1990;Ziętkiewicz et al, 1994).…”

Agromorphological and Molecular Characterization of <i>Sesamum indicum</i> L.—An Oil Seed Crop

“…The varieties were ranked with low to high seed yield as reported [28]. Sharma et al [29] reported that RAPD and ISSR markers may be converted to co-dominant sequence characterized amplified region/sequence tagged site (SCAR/STS) markers to develop variety specific markers. The present study…”

Assessment of Genetic Diversity in Indigenous Sesame Genotypes

“…However, morphological markers have limitations in their ability to estimate genetic diversity because of strong influence from environmental factors, which make them highly dependent on the cultivation conditions. Molecular marker mediated diversity analysis is considered ideal due to its abundance, hyper-variability, multi-allelic and codominant nature (Sharma et al, 2009;Yepuri et al, 2013;Wu et al, 2014;Sehr et al, 2016). Since the studies on genetic diversity analysis combining morphological and molecular markers are limited in Indian sesame germplasm, the present study was carried out to compare the genetic diversity in sesame genotypes using morphological traits and SSR markers.…”