Comparative Analysis of Proteins with Stimulating Activity on Ovarian Estradiol Biosynthesis from Four Different Dioscorea Species in vitro Using Both Phenotypic and Target-based Approaches: Implication for Treating Menopause

Lü, Jun; Wong, Ricky N S; Zhang, L.; Wong, Richard; Ng, Tzi Bun; Lee, Ckf; Zhang, Y. B.; Lao, Lixing; Liu, J. Y.; Sze, Stephen Cho Wing

doi:10.1007/s12010-016-2084-x

Cited by 11 publications

(13 citation statements)

References 39 publications

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“…In traditional medicine, wild yam is widely used to treat menopausal symptoms, most likely due to its phytoestrogen content and corresponding ability to stimulate ovarian estradiol synthesis [109][110][111]. In one study [112], twenty-four healthy postmenopausal women replaced their staple food of rice with 390 grams of yam or sweet potato (control) in two of the three meals per day for thirty days.…”

Section: Wild Yam (Dioscorea)mentioning

confidence: 99%

A Review of the Science of Colorful, Plant-Based Food and Practical Strategies for “Eating the Rainbow”

Minich

2019

Journal of Nutrition and Metabolism

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Over the past decades, thousands of published studies have amassed supporting recommendations to consume fruits and vegetables for physiological and psychological health. Newer research has emerged to suggest that these plant-based foods contain a plethora of not only vitamins and minerals, but perhaps, most importantly, phytonutrients. These phytonutrients have known pleiotropic effects on cellular structure and function, ultimately resulting in the modulation of protein kinases and subsequent epigenetic modification in a manner that leads to improved outcomes. Even though eating fruits and vegetables is a well-known feature of a healthy dietary pattern, population intakes continue to be below federal recommendations. To encourage consumers to include fruits and vegetables into their diet, an “eat by color” approach is proposed in this review. Although each individual food may have numerous effects based on its constituents, the goal of this simplified approach was to identify general patterns of benefits based on the preponderance of scientific data and known mechanisms of food-based constituents. It is suggested that such a consumer-oriented categorization of these plant-based foods may lead to greater recognition of their importance in the daily diet throughout the lifespan. Other adjunctive strategies to heighten awareness of fruits and vegetables are discussed.

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Section: Wild Yam (Dioscorea)mentioning

confidence: 99%

A Review of the Science of Colorful, Plant-Based Food and Practical Strategies for “Eating the Rainbow”

Minich

2019

Journal of Nutrition and Metabolism

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“…The active fraction of GJE and its major compounds were also investigated. With the combination of the major compounds from the active fraction and possible active ingredients from network pharmacology, phenotypic (estrogen-stimulating effect) and target-based (aromatase/FSHR/StAR/ERα/ERβ) methods were used to find the active components of GJE (Lu et al, 2016 ). Phenotypic approaches play a crucial role in drug discovery because data from the large-scale drug screening and target-based approaches suggest the relationships between a potential new drug and its molecular targets (Gilbert, 2013 ; Swamidass et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…The major components were then extracted from the bioactive fraction and converged with the probable active ingredients predicted by network pharmacology. Among these components, phenotypic (based on the estradiol assay) and target-based (based on the molecular docking analysis, Western blot assay and FSHR inhibition assay) drug-screening principles were applied to screen bioactive compounds with estrogen-stimulating effects (Hughes et al, 2011 ; Lu et al, 2016 ). Breast cancer cells were also used to pre-evaluate the cancer risk of GJE active ingredients.…”

Section: Introductionmentioning

confidence: 99%

Identification of Steroidogenic Components Derived From Gardenia jasminoides Ellis Potentially Useful for Treating Postmenopausal Syndrome

Wang

Rong

et al. 2018

Front. Pharmacol.

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Estrogen-stimulating principles have been demonstrated to relieve postmenopausal syndrome effectively. Gardenia jasminoides Ellis (GJE) is an herbal medicine possessing multiple pharmacological effects on human health with low toxicity. However, the therapeutic effects of GJE on the management of postmenopausal syndrome and its mechanism of action have not been fully elucidated. In this study, network pharmacology-based approaches were employed to examine steroidogenesis under the influence of GJE. In addition, the possibility of toxicity of GJE was ruled out and four probable active compounds were predicted. In parallel, a chromatographic fraction of GJE with estrogen-stimulating effect was identified and nine major compounds were isolated from this active fraction. Among the nine compounds, four of them were identified by network pharmacology, validating the use of network pharmacology to predict active compounds. Then the phenotypic approaches were utilized to verify that rutin, chlorogenic acid (CGA) and geniposidic acid (GA) exerted an estrogen-stimulating effect on ovarian granulosa cells. Furthermore, the results of target-based approaches indicated that rutin, CGA, and GA could up-regulate the FSHR-aromatase pathway in ovarian granulosa cells. The stimulation of estrogen production by rat ovarian granulosa cells under the influence of the three compounds underwent a decline when the follicle-stimulating hormone receptor (FSHR) was blocked by antibodies against the receptor, indicating the involvement of FSHR in the estradiol-stimulating activity of the three compounds. The effects of the three compounds on estrogen biosynthesis- related gene expression level were further confirmed by Western blot assay. Importantly, the MTT results showed that exposure of breast cancer cells to the three compounds resulted in reduction of cell viability, demonstrating the cytotoxicity of the three compounds. Collectively, rutin, chlorogenic acid and geniposidic acid may contribute to the therapeutic potential of GJE for the treatment of postmenopausal syndrome.

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“…Consistently, recent report found that glycyrrhizae radix, cinnamomi cortex, Evodiae fructus, and Zingiberis rhizoma demonstrate ER β -dependent estrogenic activity and their combined use could produce synergistic ER β -dependent estrogenic activity [ 51 ]. In addition, the estrogen-stimulating bioactive proteins isolated from Dioscorea species could upregulate the protein expression of ER β and its translational levels, potentially reducing the risk of ovarian cancer [ 52 ]. These results scientifically support the traditional use of Chinese medicine for relieving menopausal syndrome or treating other female aging disorders, and progress in analysis techniques [ 53 ] would widely spread its potential clinical use in the future.…”

Section: Discussionmentioning

confidence: 99%

Herb Formula ZhenRongDan Balances Sex Hormones, Modulates Organ Atrophy, and Restores ERα and ERβ Expressions in Ovariectomized Rats

Zhou

Chen

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Herb mixtures are widely used for treatment of the menopausal syndrome long before the hormonal therapy. However, there is insufficient data for herb remedies in treating menopausal syndromes. Here we aim to investigate the effect of ZhenRongDan (ZRD) in balancing female hormones, regulating expression of estrogen receptors (ERs), and preventing organ atrophy in menopausal rats. Rats that underwent bilateral ovariectomy were used in the experiments; the effects of ZRD on serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and estradiol (E2) levels were observed. Histology of vagina and ERs expression in vagina, uterus, and adrenal gland were also examined. ELISAs were used to analyze the changes of FSH, LH, PRL, and E2 in serum, and the morphological changes of the cervical epithelium cells were observed by Hematoxylin & Eosin (H&E) staining. Immunohistochemistry and western blot were applied to detect estrogen receptors subtypes alpha (ERα) and beta (ERβ) expression in vagina, uterus, and adrenal gland. We found that ZRD could significantly reduce the weight of the adrenal gland and increase the weight of the uterus. It could decrease the release of FSH and LH as well as increasing E2 and PRL levels. Furthermore, ZRD could improve the number of cervical vaginal epithelial cells and increase the thickness of the vaginal wall. And the altered expressions of ERα and ERβ are also restored by ZRD. ZRD could obviously relieve the endocrine disorders, modulate organ atrophy, and restore ERα and ERβ expression in the ovariectomized rat model.

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Comparative Analysis of Proteins with Stimulating Activity on Ovarian Estradiol Biosynthesis from Four Different Dioscorea Species in vitro Using Both Phenotypic and Target-based Approaches: Implication for Treating Menopause

Cited by 11 publications

References 39 publications

A Review of the Science of Colorful, Plant-Based Food and Practical Strategies for “Eating the Rainbow”

A Review of the Science of Colorful, Plant-Based Food and Practical Strategies for “Eating the Rainbow”

Identification of Steroidogenic Components Derived From Gardenia jasminoides Ellis Potentially Useful for Treating Postmenopausal Syndrome

Herb Formula ZhenRongDan Balances Sex Hormones, Modulates Organ Atrophy, and Restores ERα and ERβ Expressions in Ovariectomized Rats

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