2004
DOI: 10.1016/j.ibmb.2004.03.010
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Comparative analysis of proteinase activities of Bacillus thuringiensis-resistant and -susceptible Ostrinia nubilalis (Lepidoptera: Crambidae)

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Cited by 97 publications
(103 citation statements)
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“…The insect midgut is highly rich in proteases, and protease alteration has been observed in insects resistant to Cry1A toxins (54,55). The series of analyses of larval midgut proteases from the susceptible strain and its near-isogenic Cry2Ab-resistant T. ni strains in this study (Fig.…”
Section: Discussionmentioning
confidence: 89%
“…The insect midgut is highly rich in proteases, and protease alteration has been observed in insects resistant to Cry1A toxins (54,55). The series of analyses of larval midgut proteases from the susceptible strain and its near-isogenic Cry2Ab-resistant T. ni strains in this study (Fig.…”
Section: Discussionmentioning
confidence: 89%
“…Protease-mediated resistance mechanisms have been suggested for a number of insect species, including the Plodia interpunctella 198r population, where a reported defect in protoxin activation (25) correlated with a resistance allele closely linked to a protease null mutation (26). A resistant population of Ostrinia nubilalis has also been found to have significantly lower trypsin-like proteinase activity than a nonselected, susceptible subpopulation (15). Protoxin processing studies with this population also found a slower rate of protoxin activation although, as with the present study, the end products from resistant and susceptible populations were seemingly identical.…”
Section: Discussionmentioning
confidence: 99%
“…Cry1Ab was 125 I labeled by a chloramine-T method (38). Cry1Ac and Cry1Ca were 125 I labeled by the Iodo-Bead (Pierce, Rockford, Ill.) method (15). Specific activities of the labeled proteins were 125 I-labeled Cry1Ab ( 125 I-Cry1Ab; 0.48 mCi/mg), 125 I-Cry1Ac (0.08 mCi/mg), and 125 I-Cry1Ca (0.02 mCi/mg) as determined by an enzyme-linked immunosorbent sandwich assay (39).…”
mentioning
confidence: 99%
“…However, Bt toxins are not toxic to people, wildlife, or most beneficial insects [102,103] and therefore the opportunities for biological control are great. The effect of Bt toxins on a range of lepidopteran insects has been studied including: Bombyx mori [104], Helicoverpa armigera [105], Heliothis virescens [106,107], Manduca sexta [108,109], Ostrinia nubilalis [110][111][112][113], Plutella xylostella [114,115], Sesamia nonagrioides [115], Spodoptera exigua [116], Spodoptera frugiperda [117] and Spodoptera littoralis [118]. The Cry toxins produced in Bt crops generally target lepidopteran pests, although some also target coleopteran pests [100].…”
Section: The Bacillus Thuringiensis Endotoxinsmentioning
confidence: 99%
“…Another resistance mechanism associated with an ABC transporter locus has been reported in three lepidopteran spp (H. virescens, P. xylostella and T. ni [157]). Resistance to Bt in Ostrinia nubialis is due to reduced midgut protease activity resulting in less activation of the protoxins [111,158,159].…”
Section: Resistance Of Lepidopteran Insects To Bt Toxinsmentioning
confidence: 99%