Comparative Analysis of Protein Profiles of Malassezia Pachydermatis Strains Isolated from Healthy Dogs and Dogs with Otitis Externa

Nowakiewicz, Aneta; Ziółkowska, Grażyna

doi:10.2478/bvip-2013-0008

Cited by 4 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The external acoustic meatus represents a fragile environment in which inflammatory changes are capable of causing alterations in the skin's microbiota such that the uncontrolled proliferation of M. pachydermatis becomes a perpetuating factor of canine external otitis. By itself, this yeast is responsible for about 30 to 80% of canine otitis externa cases and is also associated with about 30% of seborrheic dermatitis and atopy cases in dogs [2] [3]. In humans, M. pachydermatis can cause fungaemias, especially in newborns that are kept in intensive therapy [4] and in immunocompromised patients; moreover, it has been observed that it can even cause alterations in immunocompetent patients, thus reveling its zoonotic potential [5].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Changes in Actin Filaments of RK13 Cells Infected with <i>Malassezia pachydermatis</i>

Rodríguez¹,

Sánchez²,

Bordes³

et al. 2018

OJVM

View full text Add to dashboard Cite

Background: Malassezia pachydermatis is the main causative agent of canine otitis and also of a myriad of dermatological problems in companion animals; its interaction mechanisms with host cells are still unclear. Objectives: To establish an in vitro infection model of M. pachydermatis-exposed RK13 cells in order to evaluate cell morphological changes as well as changes in the structure of actin filaments. Methods: Cultures of RK13 cells were infected with M. pachydermatis, alterations caused by the yeast were evaluated by optical and fluorescence microscopy. Results: M. pachydermatis adheres itself to the cell and produces the formation of multiple agglomerates that cause changes in cell morphology, formation of cell aggregates in overlays, presence of syncytia and destruction of cell culture structure. The damaged cells presented changes in the actin filaments consisting of thickening of the cell cortex and loss of stress fibers. On the other hand, the formation of perinuclear actin rings in the yeasts was observed. Conclusions: An in vitro infection model was established with M. pachydermatis and alterations in cell morphology were observed consisting of changes in the structure of the actin filaments, overgrowth of the cells and the presence of syncytia.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of Changes in Actin Filaments of RK13 Cells Infected with <i>Malassezia pachydermatis</i>

Rodríguez¹,

Sánchez²,

Bordes³

et al. 2018

OJVM

View full text Add to dashboard Cite

show abstract

Genetic relationships and population structure of Malassezia pachydermatis strains isolated from dogs with otitis externa and healthy dogs

et al. 2018

View full text Add to dashboard Cite

Malassezia pachydermatis causes infections of the skin and mucous membranes, especially in animals. It is commonly accepted that symptom manifestation depends on the physiological status of the host (different metabolic, hormonal, and immunological disorders). However, it should be considered whether distinct strains of M. pachydermatis could have different pathogenic potential and maintain opposite relations with the host, such as commensalism or parasitism. The scope of this study was to explore the population structure, genetic diversity, and phylogenetic relationships of M. pachydermatis strains isolated from dogs with clinical symptoms of otitis externa and from healthy dogs in order to investigate their relationships and evolutionary history. For all tests, a group of 30 strains derived from dogs with otitis externa and 34 strains from healthy dogs were used. The level of genetic diversity was initially assessed by polymerase chain reaction (PCR)-based random amplification of polymorphic DNA (RAPD-PCR), whereas evolutionary history was assessed by comparison of the nucleotide sequences of the internal transcribed spacer ITS1 region of nuclear rDNA. RAPD-PCR fingerprinting revealed a high level of genetic polymorphism in both tested groups (85% of unique profiles), but clinical isolates usually grouped together with other strains from otitis externa cases. Sequencing analysis identified 17 distinct genotypes with 59 polymorphic sites within both populations; however, putatively virulent strains were more closely related, indicating a probable correlation between the genotype and the virulence potential. Therefore, the hypothesis that M. pachydermatis virulence depends solely on the host's properties should be reconsidered including evolutionary and epidemiological data.

show abstract

Differences in protein profiles betweenMalassezia pachydermatisstrains obtained from healthy and infected dogs

et al. 2019

View full text Add to dashboard Cite

Comparative Analysis of Protein Profiles of Malassezia Pachydermatis Strains Isolated from Healthy Dogs and Dogs with Otitis Externa

Abstract: The study included 40 strains of Malassezia pachydermatis obtained in pure culture from external ear canal of dogs and the reference strain.

Cited by 4 publications

References 23 publications

Evaluation of Changes in Actin Filaments of RK13 Cells Infected with <i>Malassezia pachydermatis</i>

Evaluation of Changes in Actin Filaments of RK13 Cells Infected with <i>Malassezia pachydermatis</i>

Genetic relationships and population structure of Malassezia pachydermatis strains isolated from dogs with otitis externa and healthy dogs

Differences in protein profiles betweenMalassezia pachydermatisstrains obtained from healthy and infected dogs

Contact Info

Product

Resources

About

Comparative Analysis of Protein Profiles of Malassezia Pachydermatis Strains Isolated from Healthy Dogs and Dogs with Otitis Externa

Abstract: The study included 40 strains of Malassezia pachydermatis obtained in pure culture from external ear canal of dogs and the reference strain.

Cited by 4 publications

References 23 publications

Evaluation of Changes in Actin Filaments of RK13 Cells Infected with &lt;i&gt;Malassezia pachydermatis&lt;/i&gt;

Evaluation of Changes in Actin Filaments of RK13 Cells Infected with &lt;i&gt;Malassezia pachydermatis&lt;/i&gt;

Genetic relationships and population structure of Malassezia pachydermatis strains isolated from dogs with otitis externa and healthy dogs

Differences in protein profiles betweenMalassezia pachydermatisstrains obtained from healthy and infected dogs

Contact Info

Product

Resources

About

Evaluation of Changes in Actin Filaments of RK13 Cells Infected with <i>Malassezia pachydermatis</i>

Evaluation of Changes in Actin Filaments of RK13 Cells Infected with <i>Malassezia pachydermatis</i>