Comparative analysis of plant lycopene cyclases

Koç, İbrahim; Filiz, Ertuğrul; Tombuloğlu, Hüseyin

doi:10.1016/j.compbiolchem.2015.06.001

Cited by 10 publications

(10 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our comparison revealed that all these homologs share the Rossmann-fold domain for dinucleotide binding, two cyclase motifs, two transmembrane helixes, one charged region, and one uncharacterized domain with the queries (Figure 2). 24,27,28 In addition to the conserved domains, we also identified three regions that only exist in these red algal LCY homologs and the previously characterized BfLCYB (Figure 2). However, no significant similarities in these three regions were found among red algal sequences.…”

Section: ■ Resultsmentioning

confidence: 92%

Functional Characterization of Lycopene Cyclases Illustrates the Metabolic Pathway toward Lutein in Red Algal Seaweeds

Deng

Cheng

Wang

et al. 2020

J. Agric. Food Chem.

View full text Add to dashboard Cite

Carotenoids are essential phytonutrients synthesized by all photosynthetic organisms. Acyclic lycopene is the first branching point for carotenoid biosynthesis. Lycopene βand ε-cyclases (LCYB and LCYE, respectively) catalyze the cyclization of its open ends and direct the metabolic flux into different downstream branches. Carotenoids of the β,β-branch (e.g., β-carotene) are found in all photosynthetic organisms, but those of the β,ε-branch (e.g., lutein) are generally absent in cyanobacteria, heterokonts, and some red algae. Although both LCYBs and LCYEs have been characterized from land plants, there are only a few reports on LCYs from cyanobacteria and algae. Here, we cloned four LCY genes from Porphyra umbilicalis and Pyropia yezoensis (susabi-nori) of Bangiales, the most primitive red algal order that synthesizes lutein. Our functional characterization in both Escherichia coli and Arabidopsis thaliana demonstrated that each species has a pair of LCYB and LCYE. Similar to LCYs from higher plants, red algal LCYBs cyclize both ends of lycopene, and their LCYEs only cyclize a single end. The characterization of LCYEs from red algae resolved the first bifurcation step toward β-carotene and lutein biosynthesis. Our phylogenetic analysis suggests that LCYEs of the green lineage and the red algae originated separately during evolution.

show abstract

Section: ■ Resultsmentioning

confidence: 92%

Functional Characterization of Lycopene Cyclases Illustrates the Metabolic Pathway toward Lutein in Red Algal Seaweeds

Deng

Cheng

Wang

et al. 2020

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Several distinct conserved motifs were observed in DbLCYb, such as plant LCYb conserved regions, dinucleotide FAD/NAD‐binding domain (DX 4 GXGX 2 GX 3 A), cyclase motifs I and II and charged region. Cyclase motifs were essential domains involved in catalytic activity or substrate recognition (Koc et al ., ).…”

Section: Resultsmentioning

confidence: 97%

The salt‐regulated element in the promoter of lycopene β‐cyclase gene confers a salt regulatory pattern in carotenogenesis of Dunaliella bardawil

Liang

Chen

et al. 2016

Environmental Microbiology

View full text Add to dashboard Cite

In the carotenoid biosynthesis, lycopene β-cyclase (LCYb) is a key regulatory enzyme involved in the conversion of lycopene into β-carotene. Under stress conditions, such as high salinity, high light and nutrient deprivation, large amounts of β-carotene can be accumulated in Dunaliella bardawil. To study on the molecular responses of salt stress in D. bardawil is of great significance to reveal the mechanisms of salt tolerance and engineer crop plants to be salt-tolerant. In this study, the full-length coding sequence of lcyb from D. bardawil (Dblcyb, GenBank: KX218392) was isolated by transcriptome sequencing. Then, the genomic sequence, promoter and terminator regions of Dblcyb were isolated by genome walking. The Dblcyb promoter (GenBank: KX218393) contained several typical transcription boxes, multiple light response elements and a salt-regulated element (SRE, GT1GMSCAM4). Dbpsy and Dblcyb responsible for β-carotene biosynthesis in D. bardawil was shown to be up-regulated under salt stress and their promoters contained the common SRE. By element deletion analysis and using Ble-EGFP as the reporter, the salt-inducible SRE was confirmed to confer salt-induced expression of Dblcyb promoter. It was indicated that the salt-regulated expression of Dblcyb may be attributed to the salt-responsive element (GT1GMSCAM4) and the GT-rich region in its genomic sequence.

show abstract

“…“Pera”), and wolfberry [ 33 , 41 , 42 ]; lycopene β-cyclase ( LCYB ) in Arabidopsis , citrus ( Citrus unshiu Marc., Citrus sinensis Osbeck, Citrus limon Burm.f. ), I. dentate , maize, papaya, persimmon, strawberry, sweet range, and wheat [ 26 , 29 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ]; lycopene ε-cyclase ( LCYE ) in Arabidopsis , citrus ( Citrus unshiu Marc., Citrus sinensis Osbeck, Citrus limon Burm.f. ), and strawberry [ 26 , 43 , 50 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization, Expression Analysis of Carotenoid, Xanthophyll, Apocarotenoid Pathway Genes, and Carotenoid and Xanthophyll Accumulation in Chelidonium majus L.

Sathasivam

Yeo

Park

et al. 2021

Plants

View full text Add to dashboard Cite

Chelidonium majus L. is a perennial herbaceous plant that has various medicinal properties. However, the genomic information about its carotenoid biosynthesis pathway (CBP), xanthophyll biosynthesis pathway (XBP), and apocarotenoid biosynthesis pathway (ABP) genes were limited. Thus, the CBP, XBP, and ABP genes of C. majus were identified and analyzed. Among the 15 carotenoid pathway genes identified, 11 full and 4 partial open reading frames were determined. Phylogenetic analysis of these gene sequences showed higher similarity with higher plants. Through 3D structural analysis and multiple alignments, several distinct conserved motifs were identified, including dinucleotide binding motif, carotene binding motif, and aspartate or glutamate residues. Quantitative RT-PCR showed that CBP, XBP, and ABP genes were expressed in a tissue-specific manner; the highest expression levels were achieved in flowers, followed by those in leaves, roots, and stems. The HPLC analysis of the different organs showed the presence of eight different carotenoids. The highest total carotenoid content was found in leaves, followed by that in flowers, stems, and roots. This study provides information on the molecular mechanisms involved in CBP, XBP, and ABP genes, which might help optimize the carotenoid production in C. majus. The results could also be a basis of further studies on the molecular genetics and functional analysis of CBP, XBP, and ABP genes.

show abstract

Comparative analysis of plant lycopene cyclases

Cited by 10 publications

References 56 publications

Functional Characterization of Lycopene Cyclases Illustrates the Metabolic Pathway toward Lutein in Red Algal Seaweeds

Functional Characterization of Lycopene Cyclases Illustrates the Metabolic Pathway toward Lutein in Red Algal Seaweeds

The salt‐regulated element in the promoter of lycopene β‐cyclase gene confers a salt regulatory pattern in carotenogenesis of Dunaliella bardawil

Molecular Characterization, Expression Analysis of Carotenoid, Xanthophyll, Apocarotenoid Pathway Genes, and Carotenoid and Xanthophyll Accumulation in Chelidonium majus L.

Contact Info

Product

Resources

About