Comparative analysis of phenotypic and genotypic diversity among plantain landraces (Musa spp., AAB group)

Crouch, Hutokshi; Crouch, Jonathan H.; Madsen, Sten; Vuylsteke, D; Ortíz, Rodomiro

doi:10.1007/s001220051580

Cited by 53 publications

(46 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since all the plantain ecotypes were from a common genetic base (plantain AAB), Nei's genetic diversity and total heterozygosity were very low. Genetic divergence was found to be quite low within the plantain ecotypes gene pool, which is consistent with the proposed evolution of this germplasm through somatic mutation of a relatively small number of introductions (Crouch et al, 2000). However, this is in contrast to the results obtained by Mukherjee et al (2013) in analysis of some economically important species, varieties, and cultivars of the genus Allium using RAPD and ISSR markers, where they got higher values.…”

Section: Discussionsupporting

confidence: 82%

“…This revealed the existence of a sufficient amount of genetic variability among the ecotypes of the same variety, which could be exploited further in breeding programs. Crouch et al (2000) also observed large variations among plantain ecotypes (Musa spp., AAB group) using agronomic and molecular markers. These studies showed that RAPD and ISSR marker systems can be effectively used in the determination of genetic relationships among plantain ecotypes.…”

Section: Discussionmentioning

confidence: 87%

“…Random amplified polymorphic DNA (RAPD) has proved an efficient tool in detecting genetic variations and genetic relationships among germplasm of many plants, including banana (Crouch et al, 2000). It is relatively quick and inexpensive, and requires a small amount of DNA and no prior sequence information of the target genome (Solouki et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular variability of plantain ecotypes from the genus Musa (Musaceae)

Choudhary¹,

Keshavachandran²,

Menon³

et al. 2014

Turk J Bot

View full text Add to dashboard Cite

IntroductionBanana (Musa L. species (Musaceae)) is considered as an important food-fruit crop and is cultivated commercially in more than 120 countries. India is a major banana producing nation with an area of 0.79 million hectares and productivity of 28.4 metric tonnes per hectare (NHB, 2011). A wide range of diploids, triploids (2n = 3x = 33), and tetraploid cultivars/landraces of banana are known that have evolved from interspecific hybrids of the 2 wild diploid species, Musa acuminate Colla (contributing ' A' genome) and Musa balbisiana Colla (contributing 'B' genome). Despite botanical homogeneity, plantains manifest wide and unique phenotypic variability regarding plant size, bunch type, bunch and fruit orientation, fruit apex shape, pseudostem, and fruit color. Nendran (Musa AAB group) is one of the leading banana cultivars belonging to the plantain subgroup and is one of the most prized plantain varieties of India. However, biodiversity in the Nendran cultivar is complex and is represented by clones distinguishable by variation in plant stature, bunch, fruit morphology, and degree of development of the male phase (Menon et al., 2002). Therefore, the present study was carried out with the objective to characterize variability in plantain ecotypes of banana using molecular markers. The development and application of molecular markers provide powerful tools to reveal polymorphism, are robust to detect genetic variability (Simmons et al., 2007), and are not influenced by environment or developmental stages of the plant, thus making them an ideal tool for genetic relationship studies. However, the potential usefulness of Abstract: Twelve plantain ecotypes were characterized for molecular variability using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Out of the 60 RAPD and 40 ISSR primers tested, 16 RAPD and 14 ISSR primers were found polymorphic, and were used for molecular profiling of the plantain ecotypes. The average number of bands per primer, average number of polymorphic bands per primer, mean polymorphic information content (PIC) value, resolving power (Rp), and marker indices (MI) for RAPD primers were 8.63, 3.25, 0.87, 15.49, and 2.86, whereas the respective values for the ISSR assay were 7.93, 4.14, 0.85, 12.49, and 3.55. The genetic similarity coefficient was calculated using the Jaccard coefficient. The unweighted pair group method with arithmetic averages (UPGMA)-based clustering pattern remained more or less similar for RAPD, ISSR, and combined RAPD and ISSR data. Clustering was strongly supported by high bootstrap values. The genotypes Njockkon and Changalikodan (similarity coefficient > 0.94) and Manjeri Nendran (a) and Manjeri Nendran (b) (similarity coefficient > 0.89) were more closely related than the other ecotypes. The principal component analysis (PCA) showed results similar to those of the dendrogram. The results revealed that ISSR would be a better tool than RAPD for evaluation of genetic diversity in plantain ecotypes. Further, huge variab...

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular variability of plantain ecotypes from the genus Musa (Musaceae)

Choudhary¹,

Keshavachandran²,

Menon³

et al. 2014

Turk J Bot

View full text Add to dashboard Cite

show abstract

“…The significant correlations detected between the number of unshared agro-morphological traits and SSRs indicated a good concordance between molecular marker diversity and morphotype when a small number of genotypes are screened (Crouch et al, 2000). However, differences in agro-morphological traits did not imply differences in molecular data and viceversa.…”

Section: Discussionmentioning

confidence: 86%

Genetic redundancy among durum wheat accessions as assessed by SSRs and endosperm proteins

Ruíz¹,

Aguiriano²,

Giraldo

et al. 1970

Span. j. agric. res.

View full text Add to dashboard Cite

Reducing duplication in ex-situ collections is complicated and requires good quality genetic markers. This study was conducted to assess the value of endosperm proteins and SSRs for validation of potential duplicates and monitoring intra-accession variability. Fifty durum wheat (Triticum turgidum ssp. durum) accessions grouped in 23 potential duplicates, and previously characterised for 30 agro-morphological traits, were analysed for gliadin and high molecular weight glutenin (HMWG) subunit alleles, total protein, and 24 SSRs, covering a wide genome area. Similarity and dissimilarity matrices were generated based on protein and SSRs alleles. For heterogeneous accessions at gliadins the percent pattern homology (PH) between gliadin patterns and the Nei's coefficient of genetic identity (I) were computed. Eighteen duplicates identical for proteins showed none or less than 3 unshared SSRs alleles. For heterogeneous accessions PH and I values lower than 80 identified clearly off-types with more than 3 SSRs unshared. Only those biotypes differing in no more than one protein-coding locus were confirmed with SSRs. A good concordance among proteins, morphological traits, and SSR were detected. However, the discrepancy in similarity detected in some cases showed that it is advisable to evaluate redundancy through distinct approaches. The analysis in proteins together with SSRs data are very useful to identify duplicates, biotypes, close related genotypes, and contaminations.Additional key words: cereals; duplicates; ex-situ collections; molecular data; prolamins. ResumenEvaluación de la redundancia genética con microsatellites y proteínas del endospermo en accesiones de trigo duro Reducir la existencia de duplicados en las colecciones ex-situ es una tarea complicada que require el uso de buenos marcadores geneticos. En el presente trabajo, se evalúa el valor de las proteínas del endospermo y de los SSRs para la identificación de duplicados potenciales y el análisis de la variabilidad dentro de las accesiones. Se han seleccionado 50 accesiones de trigo duro (Triticum turgidum ssp. durum), agrupadas en 23 duplicados potenciales y previamente caracterizadas para 39 caracteres agro-morfológicos. Se ha analizado su composición en proteina total, alelos de gluteninas de alto peso molecular (HMWG) y 24 SSRs, cubriendo gran parte del genoma. Con los datos de los alelos de proteínas y SSRs se han generado matrices de similitud y disimilitud. En las accesiones heterogéneas para gliadinas se ha calculado el porcentaje de homología entre los patrones de gliadinas (PH) y el coeficiente de identidad genética de Nei (I). Los 18 duplicados idénticos en su composición en proteínas mostraron menos de 3 alelos SSRs diferentes. En las accesiones heterogéneas, los valores de PH e I menores de 80 identificaron a los individuos fuera de tipo, con más de 3 alelos SSRs diferentes. Solamente aquellos biotipos que diferían en no más de 1 locus proteíco se confirmaron con SSRs. Se ha detectado una buena concordancia entre los datos de prote...

show abstract

“…The Inter Simple Sequence Repeats (ISSR) are DNA-based marker techniques that has been successfully used to determine genetic diversity and relationships Musa germplasms Kaemmer et al, (1992), Howell et al, (1994, Bhat and Jarret (1995), Crouch et al, (2000), Jain et al, (2007), Racharak and Eidathong (2007), Ruangsuttapha et al, (2007), Agoreyo et al,(2008), Brown et al,(2009) and for genome identification, Howell et al, (1994), Pillay et al, (2000), analysis of Musa breeding populations, Crouch et al, (1999), detection of somaclonal variants, Grajal-Martin et al, (1998) and genetic stability, Harirah and Khalid (2006), Ray et al, (2006), Lakshmanan et al, (2007) and Venkatachalam et al,(2007). Using these techniques an unlimited number of polymorphic bands can be produced with relative ease from minute amounts of genomic DNA, Welsh and McClelland(1990), Godwin et al, (1997), Reddy et al, (2002) allowing simultaneous screening of a large number of accessions.…”

Section: Resultsmentioning

confidence: 99%