Comparative analysis of peste des petits ruminants virus tropism in Vero and Vero/SLAM cells

Abstract

A comparative study was conducted to investigate tropism of peste des petits ruminants (PPR) virus in Vero and Vero/ SLAM (signaling lymphocyte activation molecule) cells. PPR virus was isolated from tissues and blood samples of sheep and goats following second passage in both the cell line. Intensive cytopathic effects and higher antigen load (by sandwich enzyme linked immuno sorbant assay [ELISA]) were observed in Vero/SLAM cells as compared to Vero cells. A significantly higher virus titer (i.e. ranging from 4.5 to 6.5 log 10 TCID 50 /ml) was detected in Vero/SLAM than Vero cell isolates (i.e. ranging from 3.5 to 4.5 log 10 TCID 50 /ml). The present study has demonstrated that Vero cells expressing the SLAM receptors are highly efficient for isolating PPR virus (PPRV) from pathological samples, as it offers a substantial improvement including faster viral replication resulting in primary viral isolation in a shorter period of time, and higher yield of virus than traditional cell culture methodologies.

Expression of RelA, IKBα and IKKβ Genes in peste des petits Ruminants Virus Infected Cells

“…The PPRV was subjected to virus titration in 96 well microtiter plates using Vero cells through TCID 50 method (Sannat et al, 2014) by cell-ELISA (Sarkar et al, 2012). The cells were infected with PPRV at a concentration of 0.01 m.o.i.…”

Comparative Analysis of BTS-34 and Vero-76 Cell lines for Isolation of Peste des Petits Ruminants (PPR) Virus

“…All morbilliviruses including PPR virus are capable of utilizing two receptors, CD150 (Tatsuo et al, 2001) and CD46. CD150 is a glycoprotein belonging to the CD2 subset of the immunoglobulin super family and is expressed on the surface of a proportion of primary B cells, Epstein-Barr virus (EBV) transformed B cells (B95a cells), T cell clones, memory T cells, activated T cells, immature thymocytes, mature dendritic cells, and activated monocytes (Tatsuo et al, 2001) whereas, CD46 is a complement regulatory protein expressed on all cells except red blood cells (Sannat et al, 2014).…”

“…To overcome this problem, continuous cell lines are used for isolation and cultivation of morbilliviruses. The most commonly used cell line is Vero cell line (Sannat et al, 2014) but these cells do not favor efficient PPR virus isolation due to less probability of virus growth and require numerous blind passages for the development of visible cytopathic effects (CPEs) (Albayak and Alkan, 2009;Adombi et al, 2011).…”

“…The identification of SLAM receptors used preferably by wild-type PPR virus strains and other members of morbillivirus as a mode of entry and attachment revealed the importance of these receptors for isolation of virus. The present study was designed to investigate the efficiency of BTS-34 cell line expressing the bovine SLAM receptors in comparison with Vero-76 cells (Sannat et al, 2014).…”

Reverse Genetics for Peste des Petits Ruminants Virus: Current Status and Lessons to Learn from Other Non-segmented Negative-Sense RNA Viruses

“… 2008 ). Research results have led to different opinions on the growth and replication of PPRV in various cell lines (Seki et al 2003 ; Emikpe et al 2009 ; Sannat et al 2014 ; Muniraju et al 2015 ; Fakri et al 2016 ; Latif et al 2018 ). Therefore, it is critical to select a highly sensitive cell line during PPRV rescue assay; engineered cell lines that exhibit high sensitivity for PPRV growth and replication are listed in Table 1 .…”