Comparative analysis of oxygen consumption rates between cupped oyster spat of Crassostrea gigas of French, Japanese, Spanish and Taiwanese origins

Goulletquer, Philippe; Wołowicz, Maciej; Latala, Adam; Geairon, Philippe; Huvet, Arnaud; Boudry, Pierre

doi:10.1016/s0990-7440(00)86638-3

Cited by 26 publications

(17 citation statements)

References 19 publications

(19 reference statements)

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“…Further studies are needed to document potential adaptive differences between European and Asian C. angulata populations. It has been recently shown that populations from different geographical origins have significant differences in oxygen consumption (Goulletquer et al, 1999). Such differences could be of use for selective breeding programs in Europe without the zoosanitary risks associated with the introduction of exotic oysters (Berthe and .…”

Section: Resultsmentioning

confidence: 99%

Untitled

Huvet

Lapègue

Magoulas³

et al. 2000

Conservation Genetics

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The respective status of the Portuguese oyster, Crassostrea angulata, and the Pacific oyster, Crassostrea gigas, has long been a matter of controversy. Morphological and physiological similarities, homogeneity of allozyme allelic frequencies between populations of the two taxa and the demonstration of hybridization lead most authors to suggest that they should be regrouped within the same species. The risk of introgression and the present expansion of C. gigas aquaculture in Europe raises the question of the need for preservation of C. angulata in Europe, as only a few populations remain. We studied European and Asian populations of C. gigas and C. angulata using microsatellite and mitochondrial DNA markers to estimate their genetic diversity and differentiation. The analysis of genetic distances and the distribution of allelic and haplotype frequencies revealed significant genetic differences between taxa, showing two clusters: (1) C. gigas French and Japanese populations and (2) C. angulata Portuguese and Taiwanese populations. The Asian origin of the Crassostrea angulata taxa is therefore confirmed. Unlike previous studies based on allozymes, significant nuclear genome differences were noted between C. angulata and C. gigas. Despite the presumed history of the introduction of C. angulata into Southern Europe, these populations did not show any significant reduction of variability compared to Taiwanese populations. Any conservation plans for European C. angulata populations should take its non-native origin into account. They represent a valuable genetic resources for European breeding program.

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Section: Resultsmentioning

confidence: 99%

Untitled

Huvet

Lapègue

Magoulas³

et al. 2000

Conservation Genetics

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“…C. gigas shows a superior production yield in the natural environment in France (Bougrier et al 1986, Héral 1986, Parache 1989, Soletchnick et al 2002, as its growth is twice that of the Portuguese oyster (Bougrier et al 1986). Differences were also shown in terms of their ecophysiological characteristics (His 1972, Goulletquer et al 1999, Haure et al 2003. Furthermore, genetic differences have now been observed on several levels.…”

Section: Introductionmentioning

confidence: 96%

Natural hybridization between genetically differentiated populations of Crassostrea gigas and C. angulata highlighted by sequence variation in flanking regions of a microsatellite locus

Huvet¹,

Fabioux²,

McCombie³

et al. 2004

Mar. Ecol. Prog. Ser.

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The marine environment is of special interest for studying hybridization between closely related taxa because of the high dispersal potential of planktonic larvae, such as those of most bivalve species. The oysters Crassostrea angulata and C. gigas are known to be very close genetically and entirely inter-fertile under controlled conditions. However, hybridization in the wild had not been investigated, mainly due to the lack of nuclear diagnostic markers. In the present paper, we first estimated genetic differentiation between these 2 closely related taxa using 8 microsatellite markers. Interestingly, 5 markers displayed significant differences of allele size between taxa. The subsequent sequencing of alleles of one of these microsatellites showed several mutational events, which suggested null alleles and homoplasy. The presence of 1 insertion/deletion event in its 5' flanking sequence enabled us to design a new bi-allelic ('C' and 'NC') nuclear PCR-restriction fragment length polymorphism (-RFLP) marker (CG44R). This, together with a mitochondrial DNA marker, was used to analyze populations of C. angulata and C. gigas. The CG44R allele frequencies were very different between C. angulata (f[C] = 0.91) and C. gigas (f[NC] = 0.92) populations. This analysis also provided evidence for hybridization between C. angulata and C. gigas in a wild Portuguese population where the 2 taxa are in contact due to recent transportation of C. gigas stocks for aquacultural production. Our results represent the first indication of hybridization between these 2 taxa in the natural environment, and contribute to knowledge of the evolutionary history of the Crassostrea genus.

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“…Analyses of spawn from both taxa in their natural environment (Marennes-Oléron Sound) suggested non-synchronized hatching periods. Differences in their ecophysiological characteristics were also noted (His, 1972;Goulletquer et al, 1999). Moreover, a recent comparative study of ecophysiological performance showed that scope for growth, calculated non-stop over periods of several hours, was significantly greater for C. gigas than C. angulata (Haure, pers.…”

Section: Introductionmentioning

confidence: 98%

A comparative field study of growth, survival and reproduction of Crassostrea gigas, C. angulata and their hybrids

Soletchnik

Huvet

Moine

et al. 2002

Aquatic Living Resources

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This paper reports a comparative study of two cupped oysters, Crassostrea gigas and C. angulata, and their hybrids reared in the Marennes-Oléron Sound (France) under usual farming conditions. The relative proficiency of these species was characterized in terms of growth, survival and reproduction, three major concerns for oyster farming. Four populations of each taxon were sampled (C. gigas: Japan and France; C. angulata: Taiwan and Spain) and used as progenitors to produce six different progenies under common hatchery and nursery conditions (transfer of 5 to 6 g of seed oysters at sea and rearing in bags for 2 years). Mortality profiles differed greatly among the progenies. The C. angulata progeny of Taiwanese origin gave the poorest results, with 100% mortality during the second year. For the entire rearing period, the highest yield (+22%) was obtained with the C. gigas progeny of French origin. The sexual maturation index, lipid cycles and dry weight loss after spawning showed that spawning of C. angulata was delayed by about two weeks compared to that of C. gigas. A clear maternal effect was observed for the growth and reproductive characteristics of the hybrids. On the whole, C. gigas of French origin gave the best aquacultural results. However, severe episodes of C. gigas mortality have been observed in France in recent years, indicating that this species might not be entirely suitable for sustained oyster farming in this country.

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Comparative analysis of oxygen consumption rates between cupped oyster spat of Crassostrea gigas of French, Japanese, Spanish and Taiwanese origins

Cited by 26 publications

References 19 publications

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Natural hybridization between genetically differentiated populations of Crassostrea gigas and C. angulata highlighted by sequence variation in flanking regions of a microsatellite locus

A comparative field study of growth, survival and reproduction of Crassostrea gigas, C. angulata and their hybrids

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