Comparative Analysis of Olive-Derived Phenolic Compounds’ Pro-Melanogenesis Effects on B16F10 Cells and Epidermal Human Melanocytes

Abstract

Olive leaf contains plenty of phenolic compounds, among which oleuropein (OP) is the main component and belongs to the group of secoiridoids. Additionally, phenolic compounds such as oleocanthal (OL) and oleacein (OC), which share a structural similarity with OP and two aldehyde groups, are also present in olive leaves. These compounds have been studied for several health benefits, such as anti-cancer and antioxidant effects. However, their impact on the skin remains unknown. Therefore, this study aims to compare the effects of these three compounds on melanogenesis using B16F10 cells and human epidermal cells. Thousands of gene expressions were measured by global gene expression profiling with B16F10 cells. We found that glutaraldehyde compounds derived from olive leaves have a potential effect on the activation of the melanogenesis pathway and inducing differentiation in B16F10 cells. Accordingly, the pro-melanogenesis effect was investigated by means of melanin quantification, mRNA, and protein expression using human epidermal melanocytes (HEM). This study suggests that secoiridoid and its derivates have an impact on skin protection by promoting melanin production in both human and mouse cell lines.

Cyclocurcumin, a Minor Curcuminoid, Is a Novel Candidate for Hypopigmentary Skin Disorders with Melanogenesis-Stimulating Capacity

“…Our results indicate that CYC robustly increased melanin production and export (by increasing dendritic growth) in B16F10 cells, MNT-1 cells, B16F10, and MNT-1 coculture models. The rationale for selecting different cells utilized in this study is as follows: In several prior reports [59][60][61][62][63][64], including our own [39,65], compounds that promoted melanogenesis have been identified using the well-validated and widely used B16F10 murine melanoma cell model. Derived from human melanoma, the MNT-1 cell line has a transcriptome almost identical to actual human melanocytes [66] and is widely used to evaluate the effects of compounds on melanogenesis [65,67,68].…”